<scp>RNASET</scp>2 in human spermatozoa and seminal plasma: a novel relevant indicator for asthenozoospermia

Liu, Y.; Chen, G.; Lu, Liming; Sun, Hao; Guo, Qingsheng; Xue, Ke; Fan, Yong; Ding, Zhide

doi:10.1111/j.2047-2927.2012.00022.x

Cited by 13 publications

(10 citation statements)

References 33 publications

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“…Detection of cell purity was carried out by indirect immunofluorescent staining in conjunction with flow cytometry. The immunofluorescent staining and flow cytometry assay were performed as described [ 31 ]. The isolated spermatogonia were fixed in 4% paraformaldehyde for 10 min at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Expression of transcriptional factor EB (TFEB) in differentiating spermatogonia potentially promotes cell migration in mouse seminiferous epithelium

Liu

Wang

et al. 2018

Reprod Biol Endocrinol

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BackgroundSpermatogenesis is a complex process involving the self-renewal and differentiation of spermatogonia into mature spermatids in the seminiferous tubules. During spermatogenesis, germ cells migrate from the basement membrane to cross the blood-testis barrier (BTB) and finally reach the luminal side of the seminiferous epithelium. However, the mechanism for regulating the migration of germ cells remains unclear. In this study, we focused on the expression and function of transcriptional factor EB (TFEB), a master regulator of lysosomal biogenesis, autophagy and endocytosis, in spermatogenesis.MethodsThe expression pattern of the TFEB in mouse testes were investigated by Western blotting and immunohistochemistry analyses. Either undifferentiated spermatogonia or differentiating spermatogonia were isolated from testes using magnetic-activated cell sorting based on specific cell surface markers. Differentiation of spermatogonia was induced with 100 nM retinoic acid (RA). shRNA was used to knock down TFEB in cells. TFEB expression was detected by immunofluorescence, qRT-PCR, and Western blotting. Cell migration was determined by both transwell migration assay and wound healing assay applied to a cell line of immortalized spermatogonia, GC-1 cells.ResultsDuring testicular development, TFEB expression was rapidly increased in the testes at the period of 7 days post-partum (dpp) to 14 dpp, whereas in adult testis, it was predominantly localized in the nucleus of spermatogonia at stages VI to VIII of the seminiferous epithelial cycle. Accordingly, TFEB was observed to be mainly expressed in differentiating spermatogonia and was activated for nuclear translocation by RA treatment. Moreover, knockdown of TFEB expression by RNAi did not affect spermatogonial differentiation, but significantly reduced cell migration in GC-1 cells.ConclusionThese findings imply that regionally distinct expression and activation of TFEB was strongly associated with RA signaling, and therefore may promote cell migration across the BTB and transport along the seminiferous epithelium.Electronic supplementary materialThe online version of this article (10.1186/s12958-018-0427-x) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Expression of transcriptional factor EB (TFEB) in differentiating spermatogonia potentially promotes cell migration in mouse seminiferous epithelium

Liu

Wang

et al. 2018

Reprod Biol Endocrinol

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“…SDS-PAGE was conducted on 30 μg testicular protein per well using 10% denaturing polyacrylamide gels and then proteins were electrotransferred to PVDF membranes, using a semi-dry transfer apparatus [ 22 ]. Membranes were blocked for 1 h at room temperature with 5% bovine serum albumin (BSA) and immunoblotting was performed overnight at 4°C with one of the following antibodies: occludin (Invitrogen, 1:1000), ZO-1 (Invitrogen, 1:1000), androgen receptor (Santa Cruz, 1:500), clatherin (Invitrogen, 1:1000), followed by incubation with secondary antibody conjugated to HRP at a 1:8000 dilution.…”

Section: Methodsmentioning

confidence: 99%

Diet-Induced Obesity in Male C57BL/6 Mice Decreases Fertility as a Consequence of Disrupted Blood-Testis Barrier

et al. 2015

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Obesity is a complex metabolic disease that is a serious detriment to both children and adult health, which induces a variety of diseases, such as cardiovascular disease, type II diabetes, hypertension and cancer. Although adverse effects of obesity on female reproduction or oocyte development have been well recognized, its harmfulness to male fertility is still unclear because of reported conflicting results. The aim of this study was to determine whether diet-induced obesity impairs male fertility and furthermore to uncover its underlying mechanisms. Thus, male C57BL/6 mice fed a high-fat diet (HFD) for 10 weeks served as a model of diet-induced obesity. The results clearly show that the percentage of sperm motility and progressive motility significantly decreased, whereas the proportion of teratozoospermia dramatically increased in HFD mice compared to those in normal diet fed controls. Besides, the sperm acrosome reaction fell accompanied by a decline in testosterone level and an increase in estradiol level in the HFD group. This alteration of sperm function parameters strongly indicated that the fertility of HFD mice was indeed impaired, which was also validated by a low pregnancy rate in their mated normal female. Moreover, testicular morphological analyses revealed that seminiferous epithelia were severely atrophic, and cell adhesions between spermatogenic cells and Sertoli cells were loosely arranged in HFD mice. Meanwhile, the integrity of the blood-testis barrier was severely interrupted consistent with declines in the tight junction related proteins, occludin, ZO-1 and androgen receptor, but instead endocytic vesicle-associated protein, clathrin rose. Taken together, obesity can impair male fertility through declines in the sperm function parameters, sex hormone level, whereas during spermatogenesis damage to the blood-testis barrier (BTB) integrity may be one of the crucial underlying factors accounting for this change.

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“…Moreover, Skp2 was reported to be involved in the manipulation of boar sperm production [31]. The ribonuclease T2 ( RNASET2 ) gene, located in the region of 1.77–2.16 Mb on SSC1, have been reported to be involved in sperm motility impairment [32] and interacted with AKAP4 in human sperm tail and subsequently reduced sperm motility by suppressing PKA/PI3K/calcium signaling pathways [33].…”

Section: Discussionmentioning

confidence: 99%

Weighted single-step GWAS identified candidate genes associated with semen traits in a Duroc boar population

et al. 2019

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BackgroundIn the pig production industry, artificial insemination (AI) plays an important role in enlarging the beneficial impact of elite boars. Understanding the genetic architecture and detecting genetic markers associated with semen traits can help in improving genetic selection for such traits and accelerate genetic progress. In this study, we utilized a weighted single-step genome-wide association study (wssGWAS) procedure to detect genetic regions and further candidate genes associated with semen traits in a Duroc boar population. Overall, the full pedigree consists of 5284 pigs (12 generations), of which 2693 boars have semen data (143,113 ejaculations) and 1733 pigs were genotyped with 50 K single nucleotide polymorphism (SNP) array.ResultsResults show that the most significant genetic regions (0.4 Mb windows) explained approximately 2%~ 6% of the total genetic variances for the studied traits. Totally, the identified significant windows (windows explaining more than 1% of total genetic variances) explained 28.29, 35.31, 41.98, and 20.60% of genetic variances (not phenotypic variance) for number of sperm cells, sperm motility, sperm progressive motility, and total morphological abnormalities, respectively. Several genes that have been previously reported to be associated with mammal spermiogenesis, testes functioning, and male fertility were detected and treated as candidate genes for the traits of interest: Number of sperm cells, TDRD5, QSOX1, BLK, TIMP3, THRA, CSF3, and ZPBP1; Sperm motility, PPP2R2B, NEK2, NDRG, ADAM7, SKP2, and RNASET2; Sperm progressive motility, SH2B1, BLK, LAMB1, VPS4A, SPAG9, LCN2, and DNM1; Total morphological abnormalities, GHR, SELENOP, SLC16A5, SLC9A3R1, and DNAI2.ConclusionsIn conclusion, candidate genes associated with Duroc boars’ semen traits, including the number of sperm cells, sperm motility, sperm progressive motility, and total morphological abnormalities, were identified using wssGWAS. KEGG and GO enrichment analysis indicate that the identified candidate genes were enriched in biological processes and functional terms may be involved into spermiogenesis, testes functioning, and male fertility.

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RNASET2 in human spermatozoa and seminal plasma: a novel relevant indicator for asthenozoospermia

Cited by 13 publications

References 33 publications

Expression of transcriptional factor EB (TFEB) in differentiating spermatogonia potentially promotes cell migration in mouse seminiferous epithelium

Expression of transcriptional factor EB (TFEB) in differentiating spermatogonia potentially promotes cell migration in mouse seminiferous epithelium

Diet-Induced Obesity in Male C57BL/6 Mice Decreases Fertility as a Consequence of Disrupted Blood-Testis Barrier

Weighted single-step GWAS identified candidate genes associated with semen traits in a Duroc boar population

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