RNA sequencing suggests that non‐coding RNAs play a role in the development of acquired haemophilia

Abstract

Acquired haemophilia (AH) is a rare disorder characterized by bleeding in patients with no personal or family history of coagulation/clotting‐related diseases. This disease occurs when the immune system, by mistake, generates autoantibodies that target FVIII, causing bleeding. Small RNAs from plasma collected from AH patients (n = 2), mild classical haemophilia (n = 3), severe classical haemophilia (n = 3) and healthy donors (n = 2), for sequencing by Illumina, NextSeq500. Based on bioinformatic analysis, AH patients were compared to all experimental groups and a significant number of altered transcripts were identified with one transcript being modified compared to all groups at fold change level. The Venn diagram shows that haemoglobin subunit alpha 1 was highlighted to be the common upregulated transcript in AH compared to classical haemophilia and healthy patients. Non‐coding RNAs might play a role in AH pathogenesis; however, due to the rarity of HA, the current study needs to be translated on a larger number of AH samples and classical haemophilia samples to generate more solid data that can confirm our findings.

Genetics and Epigenetics in Acquired Hemophilia A: From Bench to Bedside

“…Tigu et al conducted a study in ten patients (with AH ( n = 2) with mild HA ( n = 3), with severe HA ( n = 3), and healthy controls ( n = 2)) and performed RNA sequencing analysis in the ncRNAs received from the peripheral blood. Performing bioinformatics analysis, they presented that in patients with AHA, an important number of altered transcriptomes were observed, suggesting the potential role of the ncRNAs in the pathogenesis of AHA [ 80 ]. While the study evaluated the same demographic characteristics, the small number of the participants is a limitation.…”