<scp>Phase‐separated</scp> bacterial ribonucleoprotein bodies organize <scp>mRNA</scp> decay

Muthunayake, Nisansala S.; Tomares, Dylan T.; Childers, W. Seth; Schrader, Jared M.

doi:10.1002/wrna.1599

Cited by 24 publications

(38 citation statements)

References 191 publications

(402 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, condensate disassembly is promoted by mRNA cleavage, which then releases mRNA fragments for other uses. For a more in-depth review of BR-body function and phylogenetic distribution, see Muthunayake et al (4). (21) In vitro (18) In vitro (18) In vitro (18) In vitro (22) In vitro (22) In vitro (22) In vitro (47) In vitro (23) In vivo In vitro spherical (23,24,26) In situ icosahedral (95)(96)(97)(98) In situ (25,29) In vitro (23) In vivo (91) In vitro (26) In vitro (23,24,26) ND…”

Section: Current Evidence Demonstrates That Llps May Mediate Subcellumentioning

confidence: 99%

“…Over the last decade, it has become clear that membraneless organelles play a key role in subcellular spatial organization, and a surge of studies indicates that these compartments are ubiquitous in eukaryotic cells (1). More surprisingly, recent investigations in bacteria suggest that membraneless organelles play a crucial role in the subcellular organization of bacterial cells as well (2)(3)(4)(5). However, the biochemical functions and assembly mechanisms of these compartments have not yet been completely characterized.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The emergence of phase separation as an organizing principle in bacteria

Azaldegui

Vecchiarelli

Biteen

2020

Preprint

View full text Add to dashboard Cite

Recent investigations in bacteria suggest that membraneless organelles play a crucial role in the subcellular organization of bacterial cells. However, the biochemical functions and assembly mechanisms of these compartments have not yet been completely characterized. This Review assesses the current methodologies used in the study of membraneless organelles in bacteria, highlights the limitations in determining the phase of complexes in cells that are typically an order of magnitude smaller than a eukaryotic cell, and identifies gaps in our current knowledge about the functional role of membraneless organelles in bacteria. Liquid-liquid phase separation (LLPS) is one proposed mechanism for membraneless organelle assembly. Overall, we outline the framework to evaluate LLPS in vivo in bacteria, we describe the bacterial systems with proposed LLPS activity, and we comment on the general role LLPS plays in bacteria and how it may regulate cellular function. Lastly, we provide an outlook for super-resolution microscopy and single-molecule tracking as tools to assess condensates in bacteria.Statement of SignificanceThough membraneless organelles appear to play a crucial role in the subcellular organization and regulation of bacterial cells, the biochemical functions and assembly mechanisms of these compartments have not yet been completely characterized. Furthermore, liquid-liquid phase separation (LLPS) is one proposed mechanism for membraneless organelle assembly, but it is difficult to determine subcellular phases in tiny bacterial cells. Thus, we outline the framework to evaluate LLPS in vivo in bacteria and we describe the bacterial systems with proposed LLPS activity in the context of these criteria.

show abstract

Section: Current Evidence Demonstrates That Llps May Mediate Subcellumentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The emergence of phase separation as an organizing principle in bacteria

Azaldegui

Vecchiarelli

Biteen

2020

Preprint

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

The emergence of phase separation as an organizing principle in bacteria

Azaldegui

Vecchiarelli

Biteen

2021

Biophysical Journal

125

View full text Add to dashboard Cite

Recent investigations in bacteria suggest that membraneless organelles play a crucial role in the subcellular organization of bacterial cells. However, the biochemical functions and assembly mechanisms of these compartments have not yet been completely characterized. This Review assesses the current methodologies used in the study of membraneless organelles in bacteria, highlights the limitations in determining the phase of complexes in cells that are typically an order of magnitude smaller than a eukaryotic cell, and identifies gaps in our current knowledge about the functional role of membraneless organelles in bacteria. Liquid-liquid phase separation (LLPS) is one proposed mechanism for membraneless organelle assembly. Overall, we outline the framework to evaluate LLPS in vivo in bacteria, we describe the bacterial systems with proposed LLPS activity, and we comment on the general role LLPS plays in bacteria and how it may regulate cellular function. Lastly, we provide an outlook for super-resolution microscopy and single-molecule tracking as tools to assess condensates in bacteria. Statement of SignificanceThough membraneless organelles appear to play a crucial role in the subcellular organization and regulation of bacterial cells, the biochemical functions and assembly mechanisms of these compartments have not yet been completely characterized. Furthermore, liquid-liquid phase separation (LLPS) is one proposed mechanism for membraneless organelle assembly, but it is difficult to determine subcellular phases in tiny bacterial cells. Thus, we outline the framework to evaluate LLPS in vivo in bacteria and we describe the bacterial systems with proposed LLPS activity in the context of these criteria.

show abstract

“…Thus, they are attractive tools for prokaryotes to gain spatial complexity in their cytoplasms, which are generally devoid of membrane-bound organelles. Indeed, the existence of Bacterial RNP bodies (BR-bodies) in bacteria has been recently reported ( Al-Husini et al, 2018 ; Muthunayake et al, 2020 ). In C. crescentus , RNase E assembles into BR-bodies together with the degradosome components and poorly translated RNAs, creating P-body-like condensates engaged in RNA degradation ( Al-Husini et al, 2018 , 2020 ).…”

Section: Mechanisms Potentially Enabling Uttmentioning

confidence: 99%

“…Bacteria undergo a notable metabolic slowdown upon entry into stationary phase or stress. This leads to the glassification of the cytoplasm ( Parry et al, 2014 ), which could convert BR-bodies into RNA storage condensates rather than RNA processing bodies ( Muthunayake et al, 2020 ). Such RNA-storing BR-bodies could further support UTT by accumulating and protecting untranslated transcripts until favorable conditions permit translation reinitiation.…”

Section: Mechanisms Potentially Enabling Uttmentioning

confidence: 99%

Coupled Transcription-Translation in Prokaryotes: An Old Couple With New Surprises

Irastortza-Olaziregi

Amster‐Choder

2021

Front. Microbiol.

View full text Add to dashboard Cite

Coupled transcription-translation (CTT) is a hallmark of prokaryotic gene expression. CTT occurs when ribosomes associate with and initiate translation of mRNAs whose transcription has not yet concluded, therefore forming “RNAP.mRNA.ribosome” complexes. CTT is a well-documented phenomenon that is involved in important gene regulation processes, such as attenuation and operon polarity. Despite the progress in our understanding of the cellular signals that coordinate CTT, certain aspects of its molecular architecture remain controversial. Additionally, new information on the spatial segregation between the transcriptional and the translational machineries in certain species, and on the capability of certain mRNAs to localize translation-independently, questions the unanimous occurrence of CTT. Furthermore, studies where transcription and translation were artificially uncoupled showed that transcription elongation can proceed in a translation-independent manner. Here, we review studies supporting the occurrence of CTT and findings questioning its extent, as well as discuss mechanisms that may explain both coupling and uncoupling, e.g., chromosome relocation and the involvement of cis- or trans-acting elements, such as small RNAs and RNA-binding proteins. These mechanisms impact RNA localization, stability, and translation. Understanding the two options by which genes can be expressed and their consequences should shed light on a new layer of control of bacterial transcripts fate.

show abstract

Phase‐separated bacterial ribonucleoprotein bodies organize mRNA decay

Cited by 24 publications

References 191 publications

The emergence of phase separation as an organizing principle in bacteria

The emergence of phase separation as an organizing principle in bacteria

The emergence of phase separation as an organizing principle in bacteria

Coupled Transcription-Translation in Prokaryotes: An Old Couple With New Surprises

Contact Info

Product

Resources

About