<scp>P</scp>icomolar detection of carcinoembryonic antigen in whole blood using microfluidics and surface‐enhanced Raman spectroscopy

Zou, Kun; Gao, Zhicheng; Deng, Quanfeng; Luo, Yong; Zou, Lijuan; Lu, Yao; Zhao, Wenguang; Lin, Baiquan

doi:10.1002/elps.201500535

Cited by 15 publications

(10 citation statements)

References 24 publications

(17 reference statements)

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“…Zou et al. developed protocol for detection of carcinoembryonic antigen in whole blood with the use of magnetic particles carrying capture antibody . The detection antibody and Raman‐reporter were attached to the gold nanoparticle.…”

Section: Current Application Trendsmentioning

confidence: 99%

“…Components creating a complex laminar flow pattern that enables mixing flow without the use of any external energy, e.g. zig–zag channels , serpentine channels , pillars , or branching channels belong to the most common designs.…”

Section: In‐solution Strategymentioning

confidence: 99%

“…Generally, in these applications, SERS signal from a Raman‐reporter labeling one of the selectively interacting reagents is monitored. Small organic compounds such as naphthalenethiol , mercaptobenzoic acid , bipyridine , and/or organic dyes (e.g. rhodamine, fluorescein) are usually used as Raman‐reporters giving simple and intense spectra.…”

Section: Current Application Trendsmentioning

confidence: 99%

“…At present the surface‐enhanced Raman spectroscopy covers a wide range of applications from the analysis of inorganic ions to oligomers and cell suspensions . Especially steep increase of interest has been recently observed in the field of immunoassays .…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Recent strategies toward microfluidic‐based surface‐enhanced Raman spectroscopy

2017

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Surface-enhanced Raman spectroscopy (SERS) is an extremely powerful analytical tool, which not only yields information about the molecular structure of the analyte in the form of characteristic vibrational spectrum but also gives sensitivities approaching those in fluorescence spectroscopy. The SERS measurement on the microfluidic platform provides possibility to manufacture the device with design perfectly fulfilling the needs of the application with minimal sample consumption. This review aims at describing basic strategies for SERS measurement in microfluidic devices published in the last decade and covers current trends in microfluidics with SERS detection in the field of bioanalysis and approaches toward on-line coupling of liquid-based separation techniques with SERS detection.

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Section: Current Application Trendsmentioning

confidence: 99%

Section: In‐solution Strategymentioning

confidence: 99%

Section: Current Application Trendsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Recent strategies toward microfluidic‐based surface‐enhanced Raman spectroscopy

2017

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“…Up to now the incorporation of antibody into the microfluidic-SERS system has been reported by many groups. For example, Zou et al [ 140 ] reported the CEA detection in raw blood samples exploiting microfluidics. After capturing CEA by antibody coated magnetic nanoparticle, the immunocomplex were then mixed with AuNPs coated with both antibody and Raman tag to detect CEA in whole blood with picomolar precision.…”

Section: Microfluidics-sersmentioning

confidence: 99%

Selectivity/Specificity Improvement Strategies in Surface-Enhanced Raman Spectroscopy Analysis

Wang¹,

Cao²,

Yan³

et al. 2017

Sensors

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Surface-enhanced Raman spectroscopy (SERS) is a powerful technique for the discrimination, identification, and potential quantification of certain compounds/organisms. However, its real application is challenging due to the multiple interference from the complicated detection matrix. Therefore, selective/specific detection is crucial for the real application of SERS technique. We summarize in this review five selective/specific detection techniques (chemical reaction, antibody, aptamer, molecularly imprinted polymers and microfluidics), which can be applied for the rapid and reliable selective/specific detection when coupled with SERS technique.

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Advances in Microfluidics Applied to Single Cell Operation

Zhu

Chu

Wang

2018

Biotechnology Journal

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The field of microbiology have traditionally been concerned with and focused on studies at the population level. Microfluidic platforms have emerged as important tools for biology research at a small scale, even down to a single cell level. The spatial and temporal control of cells and stimuli transported by microfluidic channels in well-designed microsystems realized the studies of specific cells in a controlled microenvironment. The true cellular physiology responses, which are obtained mostly by inference from population-level data, could be revealed in this way. Nowadays, significant applications like cell culture, analysis, sorting, genomics, and proteomics at the single cell level have been achieved in microfluidic chips. Highly integrated microfluidic systems with complete bio-analytic functions are also coming forth and of great promise for single cell related physiology, biomedical, and high throughput screening research. Herein, the leads of technologies applied to single cell operation are reviewed. Challenges and potentials of these works are also summarized, to highlight fields for further research.

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Picomolar detection of carcinoembryonic antigen in whole blood using microfluidics and surface‐enhanced Raman spectroscopy

Cited by 15 publications

References 24 publications

Recent strategies toward microfluidic‐based surface‐enhanced Raman spectroscopy

Recent strategies toward microfluidic‐based surface‐enhanced Raman spectroscopy

Selectivity/Specificity Improvement Strategies in Surface-Enhanced Raman Spectroscopy Analysis

Advances in Microfluidics Applied to Single Cell Operation

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