Recent strategies toward microfluidic‐based surface‐enhanced Raman spectroscopy

Týčová, Anna; Přikryl, Jan; Foret, František

doi:10.1002/elps.201700046

Cited by 37 publications

(21 citation statements)

References 111 publications

(176 reference statements)

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“…Advantages of microfluidic systems include preconcentration of the sample, ability to control the detection area, high reproducibility, and minimal sample volume. [132][133][134] A new microfluidic SERS platform by SERS. This method, furthermore, had higher sensitivity (70 CFU ml −1 ) and shorter detection time (less than 2 h) than a wholecell enzyme-linked immunosorbent assay (ELISA).…”

Section: Sers Combined With Microfluidicsmentioning

confidence: 99%

Surface‐enhanced Raman spectroscopy for on‐site analysis: A review of recent developments

Gong

Wang

et al. 2020

Luminescence

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On‐site identification and quantification of chemicals is critical for promoting food safety, human health, homeland security risk assessment, and disease diagnosis. Surface‐enhanced Raman spectroscopy (SERS) has been widely considered as a promising method for on‐site analysis due to the advantages of nondestructive, abundant molecular information, and outstanding sensitivity. However, SERS for on‐site application has been restricted not only by the cost, performance, and portability of portable Raman instruments, but also by the sampling ability and signal enhancing performance of the SERS substrates. In recent years, the performance of SERS for on‐site analysis has been improved through portable Raman instruments, SERS substrates, and other combined technologies. In this review, popular commercial portable Raman spectrometers and the related technologies for on‐site analysis are compared. In addition, different types of SERS substrates for on‐site application are summarized. SERS combined with other technologies, such as electrochemical and microfluidics are also presented. The future perspective of SERS for on‐site analysis is also discussed.

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Section: Sers Combined With Microfluidicsmentioning

confidence: 99%

Surface‐enhanced Raman spectroscopy for on‐site analysis: A review of recent developments

Gong

Wang

et al. 2020

Luminescence

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“…On the other hand, droplet-based microfluidics ( Figure 2 C) benefits from the immiscibility of two phases to encapsulate the SERS substrates in microdroplets (acting as microreactors) that can be analysed separately without having the diffusion effect in the flow channel, and at a high-throughput [ 53 ]. Herein, the recent advancement in microfluidic SERS devices, especially the combined forms of SERS-active nanostructures and externally injected nanomaterial are a broad area that has been reviewed elsewhere [ 26 , 28 , 64 ]. In this review, we expanded on the most recent development of label-free SERS detection in microfluidic devices using different strategies from the sensing material and microfluidics approach point of view.…”

Section: Considerationsmentioning

confidence: 99%

Surface-Enhanced Raman Scattering Spectroscopy and Microfluidics: Towards Ultrasensitive Label-Free Sensing

Kant

Abalde‐Cela

2018

Biosensors

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Raman scattering and surface-enhanced Raman scattering (SERS) spectroscopy have demonstrated their potential as ultrasensitive detection techniques in the past decades. Specifically, and as a result of the flourishing of nanotechnology, SERS is nowadays one of the most powerful sensing techniques, not only because of the low detection limits that it can achieve, but also for the structural information that it offers and its capability of multiplexing. Similarly, microfluidics technology is having an increased presence not only in fundamental research, but also in the industry. The latter is because of the intrinsic characteristics of microfluidics, being automation, high-throughput, and miniaturization. However, despite miniaturization being an advantage, it comes together with the need to use ultrasensitive techniques for the interrogation of events happening in extremely small volumes. The combination of SERS with microfluidics can overcome bottlenecks present in both technologies. As a consequence, the integration of Raman and SERS in microfluidics is being investigated for the label-free biosensing of relevant research challenges.

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“…Microfluidics in combination with SERS has gained respect as an analytical technique with attractive chemical and biological applications in order to enhance the Raman signal, such as immunoassay, 22,23 in-solution strategy, 24,25 nanoparticles embedded inside the microfluidic channel. 15,24 Nevertheless, the SERS technique must introduce external nanomaterials for Raman signal enhancement purpose, the enhanced Raman signals only provide selective structural information (typically from the Raman reporter molecules) and did not usually reflect the original signals from cells themselves. 26 In addition, metal cytotoxicity should be considered a potential problem when applying SERS nano-particles in the living cell culture.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic chip for non-invasive analysis of tumor cells interaction with anti-cancer drug doxorubicin by AFM and Raman spectroscopy

Zhang

Xiao

et al. 2018

Biomicrofluidics

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Raman spectroscopy has been playing an increasingly significant role for cell classification. Here, we introduce a novel microfluidic chip for non-invasive Raman cell natural fingerprint collection. Traditional Raman spectroscopy measurement of the cells grown in a Polydimethylsiloxane (PDMS) based microfluidic device suffers from the background noise from the substrate materials of PDMS when intended to apply as an cell assay. To overcome this disadvantage, the current device is designed with a middle layer of PDMS layer sandwiched by two MgF slides which minimize the PDMS background signal in Raman measurement. Three cancer cell lines, including a human lung cancer cell A549, and human breast cancer cell lines MDA-MB-231 and MDA-MB-231/BRMS1, were cultured in this microdevice separately for a period of three days to evaluate the biocompatibility of the microfluidic system. In addition, atomic force microscopy (AFM) was used to measure the Young's modulus and adhesion force of cancer cells at single cell level. The AFM results indicated that our microchannel environment did not seem to alter the cell biomechanical properties. The biochemical responses of cancer cells exposed to anti-cancer drug doxorubicin (DOX) up to 24 h were assessed by Raman spectroscopy. Principal component analysis over the Raman spectra indicated that cancer cells untreated and treated with DOX can be distinguished. This PDMS microfluidic device offers a non-invasive and reusable tool for Raman measurement of living cells, and can be potentially applied for anti-cancer drug screening.

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Recent strategies toward microfluidic‐based surface‐enhanced Raman spectroscopy

Cited by 37 publications

References 111 publications

Surface‐enhanced Raman spectroscopy for on‐site analysis: A review of recent developments

Surface‐enhanced Raman spectroscopy for on‐site analysis: A review of recent developments

Surface-Enhanced Raman Scattering Spectroscopy and Microfluidics: Towards Ultrasensitive Label-Free Sensing

Microfluidic chip for non-invasive analysis of tumor cells interaction with anti-cancer drug doxorubicin by AFM and Raman spectroscopy

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