MiR‐26a‐tetrahedral framework nucleic acids mediated osteogenesis of adipose‐derived mesenchymal stem cells

Abstract

Objectives: Delivery systems that provide time and space control have a good application prospect in tissue regeneration applications, as they can effectively improve the process of wound healing and tissue repair. In our experiments, we constructed a novel micro-RNA delivery system by linking framework nucleic acid nanomaterials to micro-RNAs to promote osteogenic differentiation of mesenchymal stem cells. Materials and Methods:To verify the successful preparation of tFNAs-miR-26a, the size of tFNAs-miR-26a were observed by non-denaturing polyacrylamide gel electrophoresis and dynamic light scattering techniques. The expression of osteogenic differentiation-related genes and proteins was investigated by confocal microscope, PCR and western blot to detect the impact of tFNAs-miR-26a on ADSCs. And finally, Wnt/β-catenin signaling pathway related proteins and genes were detected by confocal microscope, PCR and western blot to study the relevant mechanism.Results: By adding this novel complex, the osteogenic differentiation ability of mesenchymal stem cells was significantly improved, and the expression of alkaline phosphatase (ALP) on the surface of the cell membrane and the formation of calcium nodules in mesenchymal stem cells were significantly increased on days 7 and 14 of induction of osteogenic differentiation, respectively. Gene and protein expression levels of ALP (an early marker associated with osteogenic differentiation), RUNX2 (a metaphase marker), and OPN (a late marker) were significantly increased. We also studied the relevant mechanism of action and found that the novel nucleic acid complex promoted osteogenic differentiation of mesenchymal stem cells by activating the canonical Wnt signaling pathway.Conclusions: This study may provide a new research direction for the application of novel nucleic acid nanomaterials in bone tissue regeneration.

Methyltransferase‐like 3 modulates osteogenic differentiation of adipose‐derived stem cells in osteoporotic rats

“…Adipose‐derived stem cells (ASCs) can be easily obtained from adipose tissue and have the potential for multi‐differentiation and self‐renewal 13,14 . Therefore, ASCs are widely used in regenerative medicine and tissue engineering research 15–17 . However, the osteogenic differentiation ability of ASCs is weakened, leading to a weakening of bone repair capacity 18 .…”

“…13,14 Therefore, ASCs are widely used in regenerative medicine and tissue engineering research. [15][16][17] However, the osteogenic differentiation ability of ASCs is weakened, leading to a weakening of bone repair capacity. 18 Therefore, the key to the treatment of osteoporosis by autologous transplantation of OP-ASCs is to find a target to restore the osteogenic ability of OP-ASCs.…”

Tetrahedral Framework Nuclear Acids Can Regulate Interleukin-17 Pathway to Alleviate Inflammation and Inhibit Heterotopic Ossification in Ankylosing Spondylitis

“…Due to the source of its components, tFNAs are highly programmable and easy to modify and label with very low immunogenicity. Recently, studies have found that tFNAs can penetrate different cells to affect their various biological activities. − Moreover, our previous research has found that tFNAs or its derivatives could inhibit inflammation via regulating the IL-17 pathway. , Li and his colleagues discovered that tFNAs loaded with resveratrol could suppress inflammation and ameliorate insulin resistance via inhibiting Th1 and Th17 cell activation to reduce the production of IL-17 . In our study, tFNAs were used as a treatment option for AS, and significant therapeutic effects were observed both in vivo and in vitro .…”

Subvacuum environment‐enhanced cell migration promotes wound healing without increasing hypertrophic scars caused by excessive cell proliferation

“…This pathway is closely linked to cell migration and can promote cell migration by regulating the cytoskeleton. [23][24][25][26] In addition, Ca 2+ is an important messenger in the activation of this signalling pathway. 27,28 Hence, our findings suggest that subvacuum dressings provide a subvacuum environment for wounds, which is conducive to Ca 2+ influx, activation of the PI3K/ AKT1 signalling pathway, and regulation of the cytoskeleton.…”

“…The results showed a significant increase in the content of proteins related to the PI3K/AKT1 signalling pathway, including AKT1, p‐AKT1 (phospho Tyr326), PI3K, and p‐PI3K (phospho Y607). This pathway is closely linked to cell migration and can promote cell migration by regulating the cytoskeleton 23–26 . In addition, Ca 2+ is an important messenger in the activation of this signalling pathway 27,28 .…”