<scp>LGR6</scp> marks nephron progenitor cells

Ineveld, Ravian L. van; Margaritis, Thanasis; Kooiman, Berend A. P.; Groenveld, Femke; Ariese, Hendrikus C R; Lijnzaad, Philip; Johnson, Hannah R.; Korving, Jeroen; Wehrens, Ellen J.; Holstege, Frank C.P.; Rheenen, Jacco van; Drost, Jarno; Rios, Anne C.; Bos, Frank L.

doi:10.1002/dvdy.346

Cited by 3 publications

(2 citation statements)

References 33 publications

(49 reference statements)

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“…Maresin 1 signaling is mediated in part via engagement of the LGR6 receptor, which is expressed on numerous stem and progenitor cells, including in the skin (62), kidney (63), and mammary gland (64), and enhances proliferation, migration, and differentiation. In line with this, we observed low but detectable Lgr6 expression in primary quiescent MuSCs, with drastic transient upregulation during activation.…”

Section: Discussionmentioning

confidence: 99%

Maresin 1 Repletion Improves Muscle Regeneration After Volumetric Muscle Loss

Castor-Macias

Larouche

Wallace

et al. 2022

Preprint

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The acute traumatic or surgical loss of skeletal muscle, known as volumetric muscle loss (VML), is a devastating type of injury that results in exacerbated and persistent inflammation followed by fibrosis. The mechanisms that mediate the magnitude and duration of the inflammatory response and ensuing fibrosis after VML remain understudied and as such, the development of regenerative therapies has been limited. To address this need, we profiled how lipid mediators, which are potent regulators of the immune response after injury, varied with VML injuries that heal or result in fibrosis. We observed that non-healing VML injuries displayed increased pro-inflammatory eicosanoids and a lack of pro-resolving lipid mediators. Treatment of VML with a pro-resolving lipid mediator synthesized from docosahexaenoic acid, called Maresin 1, ameliorated fibrosis through reduction of neutrophils and macrophages and improved myogenesis, leading to enhanced recovery of muscle strength. These results expand our knowledge of the dysregulated immune response that develops after VML and identify a novel immuno-regenerative therapeutic modality in Maresin 1.

show abstract

Section: Discussionmentioning

confidence: 99%

Maresin 1 Repletion Improves Muscle Regeneration After Volumetric Muscle Loss

Castor-Macias

Larouche

Wallace

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Maresin 1 signaling is mediated in part via engagement of the LGR6 receptor, which is expressed on numerous stem and progenitor cells, including in the skin ( Huang et al, 2021 ), kidney ( van Ineveld et al, 2021 ), and mammary gland ( Blaas et al, 2016 ), and enhances proliferation, migration, and differentiation. In line with this, we observed low but detectable Lgr6 expression in primary quiescent MuSCs, with strong upregulation during activation suggesting Maresin 1 contributes to regenerative actions of MuSCs after injury by increasing proliferation.…”

Section: Discussionmentioning

confidence: 99%

Maresin 1 repletion improves muscle regeneration after volumetric muscle loss

Castor-Macias,

Larouche,

Wallace

et al. 2023

eLife

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The acute traumatic or surgical loss of skeletal muscle, known as volumetric muscle loss (VML), is a devastating type of injury that results in exacerbated and persistent inflammation followed by fibrosis. The mechanisms that mediate the magnitude and duration of the inflammatory response and ensuing fibrosis after VML remain understudied and as such, the development of regenerative therapies has been limited. To address this need, we profiled how lipid mediators, which are potent regulators of the immune response after injury, varied with VML injuries that heal or result in fibrosis. We observed that non-healing VML injuries displayed increased pro-inflammatory eicosanoids and a lack of pro-resolving lipid mediators. Treatment of VML with a pro-resolving lipid mediator synthesized from docosahexaenoic acid, called Maresin 1, ameliorated fibrosis through reduction of neutrophils and macrophages and enhanced recovery of muscle strength. These results expand our knowledge of the dysregulated immune response that develops after VML and identify a novel immuno-regenerative therapeutic modality in Maresin 1.

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LGR6 marks nephron progenitor cells

Ineveld

Margaritis

Kooiman

et al. 2021

Developmental Dynamics

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Background: Nephron progenitor cells (NPCs) undergo a stepwise process to generate all mature nephron structures. Mesenchymal to epithelial transition (MET) is considered a multistep process of NPC differentiation to ensure progressive establishment of new nephrons. However, despite this important role, to date, no marker for NPCs undergoing MET in the nephron exists.Results: Here, we identify LGR6 as a NPC marker, expressed in very early cap mesenchyme, pre-tubular aggregates, renal vesicles, and in segments of Sshaped bodies, following the trajectory of MET. By using a lineage tracing approach in embryonic explants in combination with confocal imaging and single-cell RNA sequencing, we provide evidence for the multiple fates of LGR6+ cells during embryonic nephrogenesis. Moreover, by using long-term in vivo lineage tracing, we show that postnatal LGR6+ cells are capable of generating the multiple lineages of the nephrons.Conclusions: Given the profound early mesenchymal expression and MET signature of LGR6 + cells, together with the lineage tracing of mesenchymal LGR6 + cells, we conclude that LGR6+ cells contribute to all nephrogenic segments by undergoing MET. LGR6+ cells can therefore be considered an early committed NPC population during embryonic and postnatal nephrogenesis with potential regenerative capability.

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LGR6 marks nephron progenitor cells

Cited by 3 publications

References 33 publications

Maresin 1 Repletion Improves Muscle Regeneration After Volumetric Muscle Loss

Maresin 1 Repletion Improves Muscle Regeneration After Volumetric Muscle Loss

Maresin 1 repletion improves muscle regeneration after volumetric muscle loss

LGR6 marks nephron progenitor cells

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