II. Yeast sequencing reports. Sequence analysis of a 31 kb DNA fragment from the right arm of Saccharomyces cerevisiae chromosome II

Abstract

The nucleotide sequence of a 31,352 bp fragment from chromosome II of Saccharomyces cerevisiae has been determined and analysed. The fragment originates from the right arm of chromosome II, located between the GAL7,10,1 and the PHO3,5 loci, at a distance of about 130 kb from the centromere. The sequence contains a tRNA tandem repeat and 17 open reading frames (ORFs) larger than 100 amino acids. One of them extends into adjacent DNA and is incomplete. The two tRNA genes, coding for a tRNA(asp) and a tRNA(arg), and three of the ORFs, had been sequenced previously, i.e. HSP26, SEC18, and UBC4. Four other ORFs showed similarity with yeast genes; amino acid transporter genes, the RAD54, SNF2 and STH1 family, the SPS2 gene and the bromodomain of SPT7, respectively. Two showed homology with sequences from other organisms, i.e. with a Plasmodium falciparum gene encoding a surface antigen and with a gene from Saimirine herpes virus respectively. Three ORFs, YBR0726, YBR0735 and YBR0740 are completely contained in YBR0727, YBR0734 and YBR0739 respectively, and thus probably do not represent real genes. Two ORFs, YBR0727 and YBR0745 most likely contain an intron.

Sequencing of a 40·5 kb Fragment Located on the Left Arm of Chromosome VII from Saccharomyces cerevisiae

“…The ORF map ( Figure 1C) shows that a region of about 16 kb, from YGL198c to YGL195w, presents four ORFs oriented towards the centromere, while another region of about 11 kb, from YGL182c to YGL190c, presents eight ORFs oriented towards the telomere. Similar situations, with several adjacent ORFs sharing the same orientation, are not common but still present in other genomic regions (Van der Aart et al, 1994). The main characteristics of the ORFs are summarized in Table 1.…”

Sequencing and analysis of 51 kb on the right arm of chromosome XV from Saccharomyces cerevisiae reveals 30 open reading frames

“…The sequence in position 4187742355 shares 72.9% identity in a 339 bp overlap with a yeast delta element (AC: X02417; Hauber et al, 1985). The adjacent sequence at positions 42355--42435 matches perfectly with the yeast tRNA-Asp gene from chromosome I1 (AC: X76294; Van der Aart et al, 1994). A yeast Abflp binding site, reported to be present in yeast RNA polymerase genes, is present at positions 14876-14930.…”