Nucleotide sequence analysis of a 32,500 bp region of the right arm of Saccharomyces cerevisiae chromosome IV

Brandt, Petra; Ramlow, Silke; Otto, Beate; Bloecker, Helmut

doi:10.1002/(sici)1097-0061(199601)12:1<85::aid-yea890>3.0.co;2-u

Cited by 6 publications

(3 citation statements)

References 22 publications

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“…In the present study, we have also detected a hitherto unknown protein, the product encoded by YDR055w (Brandt et al, 1996). This protein shows similarity to members of the Sps2 family (Caro et al, 1997).…”

Section: Proteins Involved In Cell Wall Constructionsupporting

confidence: 66%

Two-Dimensional analysis of proteins secreted bySaccharomyces cerevisiae regenerating protoplasts: a novel approach to study the cell wall

Pardo

Monteoliva

Pla

et al. 1999

Yeast

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Protoplasts of Saccharomyces cerevisiae incubated in regenerating conditions secrete cell wall components in order to allow the biosynthesis of this structure. During the first hours of incubation, many of these are not retained in the forming cell wall but remain in the medium. We have developed a method for collecting the secreted proteins and have analysed these by two‐dimensional electrophoresis to obtain a reference map of putative cell wall proteins. Several proteins were identified by microsequencing or immunoblotting; namely, cell wall hydrolytic enzymes, heat shock proteins, glycolytic enzymes and others. Some β‐1,3‐ and β‐1,6‐glucosylation was detected in the proteins secreted by regenerating protoplasts. Copyright © 1999 John Wiley & Sons, Ltd.

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Section: Proteins Involved In Cell Wall Constructionsupporting

confidence: 66%

Two-Dimensional analysis of proteins secreted bySaccharomyces cerevisiae regenerating protoplasts: a novel approach to study the cell wall

Pardo

Monteoliva

Pla

et al. 1999

Yeast

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“…The results obtained by our Northern blot analyses indicate that the TGL2 gene is not or is very poorly expressed under standard growth conditions. This is in line with the low codon adaptation index (0·14) of the TGL2 gene (Brandt et al, 1996). This may explain the observations made in this study that disruption of the TGL2 gene did not result in an obvious phenotype.…”

Section: Discussionsupporting

confidence: 88%

“…Analysis revealed that we had isolated a DNA fragment originating from chromosome IV (GenBank accession numbers Z49209 and X84162; Brandt et al, 1996). This was confirmed by hybridization of the yeast DNA insert to chromosomal DNA molecules separated by pulsed-field gel electrophoresis.…”

Section: Resultsmentioning

confidence: 64%

The Saccharomyces cerevisiae TGL2 gene encodes a protein with lipolytic activity and can complement an Escherichia coli diacylglycerol kinase disruptant

Heusden

Neboháčová

Overbeeke

et al. 1998

Yeast

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Escherichia coli cells with a disrupted diacylglycerol kinase gene are unable to grow on media containing arbutin due to a lethal accumulation of diacylglycerol. In order to isolate genes from the yeast Saccharomyces cerevisiae involved in diacylglycerol metabolism we complemented an E. coli diacylglycerol kinase disruptant with a yeast genomic library and transformants were selected capable of growing in the presence of arbutin. Using this method, a gene (TGL2) was isolated coding for a protein resembling lipases from Pseudomonas. After expression of the TGL2 gene in E. coli, lipolytic activity towards triacylglycerols and diacylglycerols with short‐chain fatty acids could be measured. Therefore, it is very likely that the TGL2 gene can complement the E. coli diacylglycerol kinase disruptant, because it encodes a protein that degrades the diacylglycerol accumulated after growth in the presence of arbutin. Disruption of the TGL2 gene in S. cerevisiae did not result in a detectable phenotype. The role of the Tgl2 protein in lipid degradation in yeast is still unclear. The nucleotide sequence published here has been submitted to the EMBL sequence data bank and is available under accession number X98000. © 1998 John Wiley & Sons, Ltd.

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The Gpr1/Fun34/YaaH Protein Family in the Nonconventional Yeast Yarrowia lipolytica and the Conventional Yeast Saccharomyces cerevisiae

Matthäus

Barth

2013

Yarrowia Lipolytica

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The sequencing of the genomes of several organisms was the first step in understanding genome organisation, gene function and of course life itself. Until now very much information could be gained by searching for similarities between genes or structures and finding homologies or direct orthologous genes. But with the sequencing data, more and more questions arise, which cannot be answered by simply looking the data. Although the yeast Saccharomyces cerevisiae was the first eukaryotic organism, the genome of which was sequenced, almost 14 years later there are still 900 uncharacterised and 800 dubious genes (26 % at all). The unconventional yeast Yarrowia lipolytica also harbours a high number of genes with unknown function-ca. 2,300 (35 %). One hundred seventy out of 220 Yarrowia lipolytica specific genes with no homology to other yeasts are also uncharacterised, which should carry the "differences" between the yeast species. So far only 50 industrial useful genes were characterised (proteases, lipases, esterases, etc.).Genes or gene products that do not have any known function have to be analysed in a more classical, genetical way. Especially the functions of membrane proteins which are resistant to a lot of investigation steps are only rarely elucidated. The Gpr1 protein of Yarrowia lipolytica is such an example. The function cannot be estimated by finding similarities to other proteins with known function. The more the information and phenotypic effects are available, the more complex the interacting network appears. This chapter will show the ongoing discovery of the function of the Gpr1/FUN34/YaaH protein family. This is especially shown for the Gpr1 protein from Yarrowia lipolytica and its orthologues in Saccharomyces cerevisiae. Here the different and also controversial facts are reviewed and discussed.

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Nucleotide sequence analysis of a 32,500 bp region of the right arm of Saccharomyces cerevisiae chromosome IV

Cited by 6 publications

References 22 publications

Two-Dimensional analysis of proteins secreted bySaccharomyces cerevisiae regenerating protoplasts: a novel approach to study the cell wall

Two-Dimensional analysis of proteins secreted bySaccharomyces cerevisiae regenerating protoplasts: a novel approach to study the cell wall

The Saccharomyces cerevisiae TGL2 gene encodes a protein with lipolytic activity and can complement an Escherichia coli diacylglycerol kinase disruptant

The Gpr1/Fun34/YaaH Protein Family in the Nonconventional Yeast Yarrowia lipolytica and the Conventional Yeast Saccharomyces cerevisiae

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