In Vitro Effect of 445 nm Blue Laser and 660 nm Low‐Level Laser on the Quantity and Quality of Human Gingival Fibroblasts

Abstract

Low-level laser therapy (LLLT) has several benefits in dentistry, including anti-inflammatory effects and increased proliferation. This study aimed to investigate the effect of 445 nm blue laser and 660 nm low-power laser on the quantity and quality of human gingival fibroblasts in vitro. In this in vitro experimental study, 445 nm and 660 nm lasers were irradiated on the samples six times. After examining the cells on the 7 th and 14 th days, the data were analyzed using ANOVA PASS11 and the post hoc Tukey test. The results showed the positive effect of the 660 nm laser on fibroblast proliferation. The viability on the 7 th day was the highest in the control group, lowest in the 445 nm laser group and highest in the 660 nm laser group on the 14 th day. In the morphological examination, the cells were spherical with narrow appendages in the control group, spindle-shaped with lamellipodia appendages in the 660 nm laser group and spherical with no cytoplasmic appendages in the 445 nm laser group. Evidence of necrosis and granulation phenomenon was observed in the 445 nm laser group. The use of the 660 nm low-power laser, compared with the 445 nm laser, has a positive effect on the quantity and quality of gingival fibroblasts.