HLA‐A, ‐B and ‐DRB1 allele and haplotype frequencies of 8333 Chinese Han from the Zhejiang province, China

Abstract

The distribution of human leucocyte antigen (HLA) allele and haplotype is varied among different ethnic populations. In this study, HLA-A, -B and -DRB1 allele and haplotype frequencies were determined in 8333 volunteer bone marrow donors of Zhejiang Han population using the polymerase chain reaction sequence-based typing. A total of 52 HLA-A, 96 HLA-B and 61 HLA-DRB1 alleles were found. Of these, the top three frequent alleles in HLA-A, HLA-B and HLA-DRB1 loci, respectively, were A*11:01 (24.53%), A*24:02 (17.35%), A*02:01 (11.58%); B*40:01 (15.67%), B*46:01 (11.87%), B*58:01 (9.05%); DRB1*09:01 (17.54%),DRB1*12:02 (9.64%) and DRB1*08:03 (8.65%). A total of 171 A-B-DRB1 haplotypes with a frequency of >0.1% were presented and the five most common haplotypes were A*33:03-B*58:01- DRB1*03:01, A*02:07-B*46:01-DRB1*09:01, A*30:01-B*13:02-DRB1*07:01, A*33:03-B*58:01-RB1*13:02 and A*11:01-B*15:02-DRB1*12:02. The information will be useful for selecting unrelated bone marrow donors and for anthropology studies and pharmacogenomics analysis.

HLA‐A, HLA‐B and HLA‐DRB1 allele and haplotype frequencies of 10 918 Koreans from bone marrow donor registry in Korea

“…Altogether 139 (91.4%) of the 152 alleles identified at HLA‐A, HLA‐B and HLA‐DRB1 loci were CWD alleles. We classified the alleles identified in the Japanese and the Chinese Han from the Zhejiang Province (Ikeda et al ., ; He et al ., ) and found similar findings as those in the present study. In these two populations, 86.4%, 85.3% and 92.9%, and 86.5%, 86.5% and 93.4% of alleles identified at HLA‐A, HLA‐B and HLA‐DRB1 locus, respectively, belonged to CWD alleles.…”

“…The numbers of alleles identified are similar to those found in a previous study of sequence‐based typing on 4128 cord blood units from Koreans (Huh et al ., ), in which a total of 44, 67 and 48 alleles were identified for HLA‐A, HLA‐B and HLA‐DRB1 loci, respectively. The diversity of HLA alleles in Koreans is comparable to that in the Japanese ( n = 19,813 haplotypes; 44 A, 75 B, 42 DRB1 alleles) and is less diverse than those in the Chinese Han from northern China ( n = 618; 84 A, 143 B, 122 DRB1 alleles) or from the Zhejiang Province, located in mideast coastal region of China ( n = 8333; 52 A, 96 B, 61 DRB1 alleles) (Yang et al ., ; Ikeda et al ., ; He et al ., ).…”

The novel HLA‐A*02:625 allele was identified in a Chinese bone marrow donor

“…Currently, more than 17 695 HLA alleles have been identified according to the IPD‐IMGT/HLA Database release 3.31.0 (January 2018), including 4081 HLA‐A alleles . It was reported that the frequency of HLA‐A*02 allele was more than 30% in the Chinese Han population . Here, we describe a novel allele HLA‐A*02:625 which has been identified in a Chinese bone marrow donor.…”

Characterization of three new HLA Class I Alleles in Chinese individuals, HLA‐B*46:68,‐B*46:71,‐B*46:72

“…However, the novel HLA alleles are still increased with molecular methods widely application in the laboratory, such as polymerase chain reaction sequence‐based typing method (PCR‐SBT) and next‐generation sequence method (Alizadeh et al, ; Profaizer, Lázár‐Molnár, Pole, Delgado, & Kumánovics, ). It was reported that HLA‐B*46 is common in the Chinese Han population with frequency 11.87% (He et al, ), and now 99 different HLA‐B*46 alleles have been identified around the world (Robinson et al, ). Here, we describe three new HLA‐B*46 alleles that have been found in Chinese individuals.…”