<scp>HGF</scp> and <scp>IGF</scp>‐1 promote protective effects of allogeneic <scp>BMSC</scp> transplantation in rabbit model of acute myocardial infarction

Zhang, Guang-Wei; Gu, Tingting; Guan, Xiaoyu; Sun, Xuejun; Qi, Xun; Li, Xueyuan; Wang, Xiaobing; Lv, Feng; Yu, Lei; Jiang, Daqing; Tang, Rui

doi:10.1111/cpr.12219

Cited by 43 publications

(26 citation statements)

References 28 publications

(32 reference statements)

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“…IGF-1 is an ubiquitous peptide hormone and an important anti-apoptotic factor (35). IGFBPs constitute multiple proteins that bind to IGFs and modulate the interaction of IGFs with their receptors (36).…”

Section: Discussionmentioning

confidence: 99%

Insulin-like growth factor-1 promotes the proliferation and odontoblastic differentiation of human dental pulp cells under high glucose conditions

Lü

Sun

Liu

2017

International Journal of Molecular Medicine

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Insulin-like growth factor-1 (IGF-1) promotes human dental pulp stem cell proliferation and osteogenic differentiation. However, the effects of IGF-1 on the proliferation, apoptosis and odontoblastic differentiation (mineralization) of dental pulp cells (DPCs) under high glucose (GLU) conditions remain unclear. In this study, isolated primary human DPCs were treated with various concentrations of high GLU. Cell proliferation and apoptosis were determined by Cell Counting Kit-8 and Annexin V-FITC/PI assays, respectively. The cells were cultured in odontoblastic induction medium containing various concentrations of high GLU. Odontoblastic differentiation was determined by alkaline phosphatase (ALP) activity assay. Mineralization formation was evaluated by von Kossa staining. The expression levels of IGF family members were measured by western blot analysis and RT-qPCR during proliferation and differentiation. The cells were then exposed to 25 mM GLU and various concentrations of IGF-1. Cell proliferation, apoptosis, ALP activity, mineralization formation and the levels of mineralization-related proteins were then evaluated. Our results revealed that high GLU significantly inhibited cell proliferation and promoted cell apoptosis. GLU (25 and 50 mM) markedly reduced ALP activity and mineralization on days 7 and 14 after differentiation. The levels of IGF family members were markedly decreased by high GLU during proliferation and differentiation. However, IGF-1 significantly reversed the effects of high GLU on cell proliferation and apoptosis. Additionally, IGF-1 markedly restored the reduction of ALP activity and mineralization induced by high GLU. Our findings thus indicate that IGF-1 attenuates the high GLU-induced inhibition of DPC proliferation, differentiation and mineralization.

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Section: Discussionmentioning

confidence: 99%

Insulin-like growth factor-1 promotes the proliferation and odontoblastic differentiation of human dental pulp cells under high glucose conditions

Lü

Sun

Liu

2017

International Journal of Molecular Medicine

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“…Importantly, the treatment resulted in the decreased production of several pro-inflammatory cytokines, namely tumor necrosis factor-α, interleukin-1β (IL-1β), and IL-6 in the infarction area. One limitation of the IGF-1 pretreatment is that it is not clear whether this GF can promote differentiation of MSCs into cardiomyocytes [ 59 ]. On the other hand, HGF is a known regulator of MSCs differentiation into cardiomyocytes [ 60 ].…”

Section: Stem Cell Preconditioning For MI Treatmentmentioning

confidence: 99%

“…On the other hand, HGF is a known regulator of MSCs differentiation into cardiomyocytes [ 60 ]. Therefore, Zhang and colleagues attempted to complement the IGF-1 preconditioning of stem cells with HGF pretreatment [ 59 ]. MSCs preconditioned with the combination of the two growth factors differentiated into cardiomyocytes both in vitro and in a rabbit model of MI.…”

Section: Stem Cell Preconditioning For MI Treatmentmentioning

confidence: 99%

Preconditioned and Genetically Modified Stem Cells for Myocardial Infarction Treatment

Raziyeva

Smagulova

Kim

et al. 2020

IJMS

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Ischemic heart disease and myocardial infarction remain leading causes of mortality worldwide. Existing myocardial infarction treatments are incapable of fully repairing and regenerating the infarcted myocardium. Stem cell transplantation therapy has demonstrated promising results in improving heart function following myocardial infarction. However, poor cell survival and low engraftment at the harsh and hostile environment at the site of infarction limit the regeneration potential of stem cells. Preconditioning with various physical and chemical factors, as well as genetic modification and cellular reprogramming, are strategies that could potentially optimize stem cell transplantation therapy for clinical application. In this review, we discuss the most up-to-date findings related to utilizing preconditioned stem cells for myocardial infarction treatment, focusing mainly on preconditioning with hypoxia, growth factors, drugs, and biological agents. Furthermore, genetic manipulations on stem cells, such as the overexpression of specific proteins, regulation of microRNAs, and cellular reprogramming to improve their efficiency in myocardial infarction treatment, are discussed as well.

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“…Activation of cardioprotective cytokines has been shown during tissue regeneration to promote antifibrosis via induction of metalloproteases (MMP) to degrade ECM, inhibition of the transition from interstitial fibroblasts to myofibroblasts and against proliferation of collagen production of fibroblasts (16). Hepatocyte growth factor (HGF) and insulinlike growth factor (IGF1) have been reported as beneficial agents to prevent apoptosis and fibrosis in cardiovascular diseases (17), liver diseases (18) and pulmonary diseases (19). Sonic hedgehog (SHH) plays a role in the development of the heart blood vessels in the embryonic heart and adult heart (20) and has potential in cardiac repair after myocardial ischemia (21).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of anti-fibrotic factors ameliorates anti-fibrotic properties of Wharton's jelly derived mesenchymal stem cells under oxidative damage

Nimsanor

Phetfong

Kitiyanant

et al. 2019

BST

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Transplantation with Wharton's jelly derived mesenchymal stem cells (WJ-MSCs) showed great benefits for restoring myocardial function. However, the outcome of WJ-MSCs transplantation was unsuccessful due to multiple factors including oxidative damage. The presence of oxidative stress due to myocardium injury influences fibrous tissue formation, which causes disability of cardiac muscle. Hepatocyte growth factor (HGF), insulin-like growth factor (IGF1), and sonic hedgehog (SHH) are well-known master regulators in antifibrosis when secreted by WJ-MSCs. They showed a beneficial role in the recovery of cardiac fibrosis after WJ-MSCs transplantation. This study hypothesizes whether the reduction of the anti-fibrosis property in WJ-MSCs from oxidative damage can be recovered by overexpression of the HGF, IGF1, or SHH gene. Overexpression was attained by transfection of WJ-MSCs with pCMV3-HGF, pCMV3-IGF1, or pCMV3-SHH followed by H 2 O 2 exposure and co-culturing with cardiac fibroblasts. Myofibroblast specific markers comprised of alpha-smooth muscle actin (α-SMA) and collagen type 1 (COL1) were evaluated. The WJ-MSCs treated with H 2 O 2 influenced the expression of myofibroblastic markers, whereas the overexpression of HGF, IGF1 or SHH reduced myofibroblastic formation. These results indicate that the oxidative stress impaired anti-fibrotic property of WJ-MSCs, leads to an increase of myofibroblasts. Overexpression of anti-fibrotic genes restored the endogenous HGF, IGF1, and SHH alleviating improvement of cardiac function.

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HGF and IGF‐1 promote protective effects of allogeneic BMSC transplantation in rabbit model of acute myocardial infarction

Abstract: Combination of HGF and IGF-1 activated BMSCs complementarily, and controlled release of the two factors promoted protective potential of transplanted BMSCs to repair infarcted myocardium. This suggests a new strategy for cell therapies to overcome acute ischemic myocardial injury.

Cited by 43 publications

References 28 publications

Insulin-like growth factor-1 promotes the proliferation and odontoblastic differentiation of human dental pulp cells under high glucose conditions

Insulin-like growth factor-1 promotes the proliferation and odontoblastic differentiation of human dental pulp cells under high glucose conditions

Preconditioned and Genetically Modified Stem Cells for Myocardial Infarction Treatment

Overexpression of anti-fibrotic factors ameliorates anti-fibrotic properties of Wharton's jelly derived mesenchymal stem cells under oxidative damage

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