Insulin-like growth factor-1 promotes the proliferation and odontoblastic differentiation of human dental pulp cells under high glucose conditions

Lü, Yan; Sun, Siyu; Liu, Qu

doi:10.3892/ijmm.2017.3117

Cited by 15 publications

(9 citation statements)

References 42 publications

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“…A heat map of the key transcripts for the osteo/dentinogenic differentiation process is shown in Figure 6E. These genes were consistent with those related to osteo/dentinogenic differentiation that were identified in previous reports [22][23][24][25][26][27][28][29][30][31][32][33].…”

Section: Rna Sequence Analysissupporting

confidence: 87%

Performance of a Biodegradable Composite with Hydroxyapatite as a Scaffold in Pulp Tissue Repair

et al. 2020

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Vital pulp therapy is an important endodontic treatment. Strategies using growth factors and biological molecules are effective in developing pulp capping materials based on wound healing by the dentin-pulp complex. Our group developed biodegradable viscoelastic polymer materials for tissue-engineered medical devices. The polymer contents help overcome the poor fracture toughness of hydroxyapatite (HAp)-facilitated osteogenic differentiation of pulp cells. However, the composition of this novel polymer remained unclear. This study evaluated a novel polymer composite, P(CL-co-DLLA) and HAp, as a direct pulp capping carrier for biological molecules. The biocompatibility of the novel polymer composite was evaluated by determining the cytotoxicity and proliferation of human dental stem cells in vitro. The novel polymer composite with BMP-2, which reportedly induced tertiary dentin, was tested as a direct pulp capping material in a rat model. Cytotoxicity and proliferation assays revealed that the biocompatibility of the novel polymer composite was similar to that of the control. The novel polymer composite with BMP-2-induced tertiary dentin, similar to hydraulic calcium-silicate cement, in the direct pulp capping model. The BMP-2 composite upregulated wound healing-related gene expression compared to the novel polymer composite alone. Therefore, we suggest that novel polymer composites could be effective carriers for pulp capping.

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Section: Rna Sequence Analysissupporting

confidence: 87%

Performance of a Biodegradable Composite with Hydroxyapatite as a Scaffold in Pulp Tissue Repair

et al. 2020

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“…They did not specify the sample that they used to acquire the DPSCs. Shunro Miyashita et al [ 9 ] and Yan et al [ 10 ] on the other hand used both first premolar and third molar for their studies. There is no inflamed pulp tissue used in any of the studies, even though Alongi et al [ 11 ] reported that it is an appropriate source for isolation of DPSCs.…”

Section: Discussionmentioning

confidence: 99%

“…However, IGF-1 significantly reversed the effects of high GLU. IGF-1 restored ALP activity and promoted odontoblastic differentiation by increasing the expression levels of mineralization-related proteins as mentioned above [ 10 ].…”

Section: Discussionmentioning

confidence: 99%

Mesenchymal stem cells: A comprehensive methods for odontoblastic induction

et al. 2021

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Background In the area of oral and maxillofacial surgery, regenerative endodontics aims to present alternative options to conventional treatment strategies. With continuous advances in regenerative medicine, the source of cells used for pulp tissue regeneration is not only limited to mesenchymal stem cells as the non-mesenchymal stem cells have shown capabilities too. In this review, we are systematically assessing the recent findings on odontoblastic differentiation induction with scaffold and non-scaffold approaches. Methods A comprehensive search was conducted in Pubmed, and Scopus, and relevant studies published between 2015 and 2020 were selected following the PRISMA guideline. The main inclusion criteria were that articles must be revolving on method for osteoblast differentiation in vitro study. Therefore, in vivo and human or animal clinical studies were excluded. The search outcomes identified all articles containing the word “odontoblast”, “differentiation”, and “mesenchymal stem cell”. Results The literature search identified 99 related studies, but only 11 articles met the inclusion criteria. These include 5 odontoblastic differentiation induction with scaffold, 6 inductions without scaffolds. The data collected were characterised into two main categories: type of cells undergo odontoblastic differentiation, and odontoblastic differentiation techniques using scaffolds or non-scaffold. Conclusion Based on the data analysis, the scaffold-based odontoblastic induction method seems to be a better option compared to the non-scaffold method. In addition of that, the combination of growth factors in scaffold-based methods could possibly enhance the differentiation. Thus, further detailed studies are still required to understand the mechanism and the way to enhance odontoblastic differentiation.

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“…Ma et al [ 163 ] further revealed the role of the IGF-1/IGF-1R/hsa-let-7c axis in regulating the committed differentiation of SCAPs. Yan et al [ 164 ] reported that IGF-1 rescued the adverse effects of high glucose concentration on DPSCs and protected against apoptosis. Regarding periodontal ligament regeneration, IGF-1 was shown to enhance the survival of PDLSCs[ 165 ].…”

Section: Cell Function Optimization Strategiesmentioning

confidence: 99%

Priming strategies for controlling stem cell fate: Applications and challenges in dental tissue regeneration

Zhang¹,

Ren²,

Yang³

2021

WJSC

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Mesenchymal stromal cells (MSCs) have attracted intense interest in the field of dental tissue regeneration. Dental tissue is a popular source of MSCs because MSCs can be obtained with minimally invasive procedures. MSCs possess distinct inherent properties of self-renewal, immunomodulation, proangiogenic potential, and multilineage potency, as well as being readily available and easy to culture. However, major issues, including poor engraftment and low survival rates in vivo , remain to be resolved before large-scale application is feasible in clinical treatments. Thus, some recent investigations have sought ways to optimize MSC functions in vitro and in vivo . Currently, priming culture conditions, pretreatment with mechanical and physical stimuli, preconditioning with cytokines and growth factors, and genetic modification of MSCs are considered to be the main strategies; all of which could contribute to improving MSC efficacy in dental regenerative medicine. Research in this field has made tremendous progress and continues to gather interest and stimulate innovation. In this review, we summarize the priming approaches for enhancing the intrinsic biological properties of MSCs such as migration, antiapoptotic effect, proangiogenic potential, and regenerative properties. Challenges in current approaches associated with MSC modification and possible future solutions are also indicated. We aim to outline the present understanding of priming approaches to improve the therapeutic effects of MSCs on dental tissue regeneration.

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Insulin-like growth factor-1 promotes the proliferation and odontoblastic differentiation of human dental pulp cells under high glucose conditions

Cited by 15 publications

References 42 publications

Performance of a Biodegradable Composite with Hydroxyapatite as a Scaffold in Pulp Tissue Repair

Performance of a Biodegradable Composite with Hydroxyapatite as a Scaffold in Pulp Tissue Repair

Mesenchymal stem cells: A comprehensive methods for odontoblastic induction

Priming strategies for controlling stem cell fate: Applications and challenges in dental tissue regeneration

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