<scp>DYT</scp>1 directly regulates the expression of <i><scp>TDF</scp>1</i> for tapetum development and pollen wall formation in <scp>A</scp>rabidopsis

Gu, Jing-Nan; Zhu, Jun; Yu, Yu; Teng, Xiaodong; Lou, Yue; Xu, Xiaofeng; Liu, Jiali; Yang, Zhong‐Nan

doi:10.1111/tpj.12694

Cited by 131 publications

(123 citation statements)

References 57 publications

Supporting

Mentioning

119

Contrasting

Order By: Relevance

“…Previous studies have revealed that DYT1 targets the promoters of MS1 and MYB35 (Feng et al, 2012;Gu et al, 2014). However, our in vivo transcriptional activation assay using the transient transcription assay system (Hellens et al, 2005;Liu et al, 2008) showed that DYT1 alone activates the expression of MS1 and MYB35 only moderately (LUC/REN<10) ( Figures 6A and 6B).…”

Section: Myb35 Expressionmentioning

confidence: 59%

“…As one of the gatekeeper TFs required for tapetum development, DYT1 is specifically expressed in tapetal cells (Gu et al, 2014). Previously, we showed (Feng et al, 2012) that expression of DYT1 in the cytoplasm as a fusion to the ligand binding domain of the glucocorticoid receptor (GR) failed to rescue the dyt1-1 mutant, with few pollen grains, but the translocation of DYT1-GR into the nucleus due to the treatment with the glucocorticoid hormone analog dexamethasone restores male fertility of ProDYT1:DYT1-GR/dyt1-1 plants.…”

Section: Resultsmentioning

confidence: 99%

“…Plant bHLH proteins play important roles in many developmental processes, including anther development (Zhang et al, 2006;Feng et al, 2012;Fu et al, 2014;Gu et al, 2014;Zhu et al, 2015), stomata development (Kanaoka et al, 2008;Bhave et al, 2009;Nadeau, 2009;Serna, 2009bSerna, , 2009a, hormone signaling (Friedrichsen et al, 2002;Zhang et al, 2009), and light responses (Castillon et al, 2007;Leivar et al, 2008;Liu et al, 2008;Shin et al, 2009;Zhang et al, 2013). TFs can form heteromeric complexes that increase their combinatorial spectrum and specificity for distinct binding sites in both animals and plants (Lamb and McKnight, 1991;Zhao et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Feedback Regulation of DYT1 by Interactions with Downstream bHLH Factors Promotes DYT1 Nuclear Localization and Anther Development

et al. 2016

View full text Add to dashboard Cite

Transcriptional regulation is one of the most important mechanisms controlling development and cellular functions in plants and animals. The Arabidopsis thaliana bHLH transcription factor (TF) DYSFUNCTIONL TAPETUM1 (DYT1) is required for normal male fertility and anther development and activates the expression of the bHLH010/bHLH089/bHLH091 genes. Here, we showed that DYT1 is localized to both the cytoplasm and nucleus at anther stage 5 but specifically to the nucleus at anther stage 6 and onward. The bHLH010/bHLH089/bHLH091 proteins have strong nuclear localization signals, interact with DYT1, and facilitate the nuclear localization of DYT1. We further found that the conserved C-terminal BIF domain of DYT1 is required for its dimerization, nuclear localization, transcriptional activation activity, and function in anther development. Interestingly, when the BIF domain of DYT1 was replaced with that of bHLH010, the DYT1 N -bHLH010 BIF chimeric protein shows nuclearpreferential localization at anther stage 5 but could not fully rescue the dyt1-3 phenotype, suggesting that the normal spatiotemporal subcellular localization of DYT1 is important for DYT1 function and/or that the BIF domains from different bHLH members might be functionally distinct. Our results support an important positive feedback regulatory mechanism whereby downstream TFs increase the function of an upstream TF by enhancing its nucleus localization through the BIF domain.

show abstract

Section: Myb35 Expressionmentioning

confidence: 59%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Feedback Regulation of DYT1 by Interactions with Downstream bHLH Factors Promotes DYT1 Nuclear Localization and Anther Development

et al. 2016

View full text Add to dashboard Cite

show abstract

“…For example, CYP703A3, encoding a cytochrome P450 fatty acid hydroxylase, can be regulated by GAMYB and TDR and can be involved in tapetal degradation . In Arabidopsis, DYSFUNCTIONAL TAPETUM1 (Gu et al, 2014), ABORTED MICROSPORES (Xu et al, 2010, and MS1 (Wilson et al, 2001;Ito et al, 2007;Yang et al, 2007) can regulate multiple genes involved in pollen wall development. Thus, we hypothesized that IPE1 indirectly regulates tapetal degeneration via transcription factors.…”

Section: Ipe1 Mediates the Lipid Metabolic Pathway In The Tapetummentioning

confidence: 99%

IRREGULAR POLLEN EXINE1 Is a Novel Factor in Anther Cuticle and Pollen Exine Formation

et al. 2016

View full text Add to dashboard Cite

ORCID IDs: 0000-0002-4656-3189 (H.S.); 0000-0003-0518-5924 (C.Z.); 0000-0001-9320-9628 (W.J.).Anther cuticle and pollen exine are protective barriers for pollen development and fertilization. Despite that several regulators have been identified for anther cuticle and pollen exine development in rice (Oryza sativa) and Arabidopsis (Arabidopsis thaliana), few genes have been characterized in maize (Zea mays) and the underlying regulatory mechanism remains elusive. Here, we report a novel male-sterile mutant in maize, irregular pollen exine1 (ipe1), which exhibited a glossy outer anther surface, abnormal Ubisch bodies, and defective pollen exine. Using map-based cloning, the IPE1 gene was isolated as a putative glucose-methanolcholine oxidoreductase targeted to the endoplasmic reticulum. Transcripts of IPE1 were preferentially accumulated in the tapetum during the tetrad and early uninucleate microspore stage. A biochemical assay indicated that ipe1 anthers had altered constituents of wax and a significant reduction of cutin monomers and fatty acids. RNA sequencing data revealed that genes implicated in wax and flavonoid metabolism, fatty acid synthesis, and elongation were differentially expressed in ipe1 mutant anthers. In addition, the analysis of transfer DNA insertional lines of the orthologous gene in Arabidopsis suggested that IPE1 and their orthologs have a partially conserved function in male organ development. Our results showed that IPE1 participates in the putative oxidative pathway of C16/C18 v-hydroxy fatty acids and controls anther cuticle and pollen exine development together with MALE STERILITY26 and MALE STERILITY45 in maize.Male sterility is a common biological phenomenon in plants and widely used in the production of hybrid seeds, which can reduce costs and enhance seed purity (Tester and Langridge, 2010). According to inheritance or origin, male sterility includes three types: genic male sterility, cytoplasmic male sterility, and cytoplasmicgenic male sterility (Rhee et al., 2015). The generation of mature pollen grains relies on anther development. The start of anther formation occurs in differentiated flower tissues (floral meristem), which consist of three histogenic layers: L1, L2, and L3. After continuous cell division and differentiation, L1 forms the epidermis and the L3 layer develops into the stomium and vascular bundles. L2 is the most important layer; it undergoes a series of periclinal and anticlinal divisions and eventually grows into the endothecium, the middle layer, the tapetum, and the pollen mother cells. When anther morphogenesis is completed, the anther has centrally localized pollen mother cells enclosed by four somatic layers, which are, from the surface to the interior, the epidermis, endothecium, middle layer, and tapetum. Then, the pollen mother cells undergo meiosis and mitosis, resulting in trinucleate pollen grains, and the endothecium, middle layer, and tapetum are gradually degraded (Goldberg et al., 1993(Goldberg et al., , 1995Ma, 2005).The anther cuticle and poll...

show abstract

“…The basic helix-loop-helix (bHLH) transcriptional factor DYSFUNCTIONAL TAPETUM1 (DYT1) is among the earliest acting male-specific regulators and is required for the normal development of tapetum by regulating the normal expression of more than 1,000 anther genes, and dyt1 mutants form abnormal prematurely vacuolated tapetal cells and lack mature pollen (Zhang et al, 2006;Feng et al, 2012;Gu et al, 2014;Zhu et al, 2015). In addition, bHLH010, bHLH089, and bHLH091 together are required for normal tapetum development and transcriptome and pollen fertility, forming both feed-forward and feedback loops with DYT1 (Zhu et al, 2015;Cui et al, 2016).…”

mentioning

confidence: 99%

Cytological and Transcriptomic Analyses Reveal Important Roles of CLE19 in Pollen Exine Formation

Wang

Song

et al. 2017

Plant Physiol.

View full text Add to dashboard Cite

The CLAVATA3/ESR-RELATED (CLE) peptide signals are required for cell-cell communication in several plant growth and developmental processes. However, little is known regarding the possible functions of the CLEs in the anther. Here, we show that a T-DNA insertional mutant, and dominant-negative (DN) and overexpression (OX) transgenic plants of the CLE19 gene, exhibited significantly reduced anther size and pollen grain number and abnormal pollen wall formation in Arabidopsis (Arabidopsis thaliana). Interestingly, the DN-CLE19 pollen grains showed a more extensively covered surface, but CLE19-OX pollen exine exhibited clearly missing connections in the network and lacked separation between areas that normally form the lacunae. With a combination of cell biological, genetic, and transcriptomic analyses on cle19, DN-CLE19, and CLE19-OX plants, we demonstrated that CLE19-OX plants produced highly vacuolated and swollen aborted microspores (ams)-like tapetal cells, lacked lipidic tapetosomes and elaioplasts, and had abnormal pollen primexine without obvious accumulation of sporopollenin precursors. Moreover, CLE19 is important for the normal expression of more than 1,000 genes, including the transcription factor gene AMS, 280 AMS-downstream genes, and other genes involved in pollen coat and pollen exine formation, lipid metabolism, pollen germination, and hormone metabolism. In addition, the DN-CLE19(+/+) ams(2/2) plants exhibited the ams anther phenotype and ams(+/2) partially suppressed the DN-CLE19 transgeneinduced pollen exine defects. These findings demonstrate that the proper amount of CLE19 signal is essential for the normal expression of AMS and its downstream gene networks in the regulation of anther development and pollen exine formation.Pollen grains are generated in the male reproductive organ anther of flowering plants and are essential for plant fertility. In the model plant Arabidopsis (Arabidopsis thaliana), pollen grains are ellipsoidal, and the pollen surface is covered by a reticulate exine. In contrast, rice (Oryza sativa) pollen grains are globular and have a smooth surface without the reticulate structure. A well-organized pollen wall structure is essential for the physical and chemical stability of the mature pollen by providing protection for pollen grains from environmental stresses, such as desiccation and microbial attacks (Scott et al., 2004). The pollen wall is composed mainly of three layers: the intine layer, the exine layer, and the pollen coat (Zinkl et al., 1999;Edlund et al., 2004;Blackmore et al., 2007). The intine is the innermost layer of the pollen wall immediately adjacent to the plasma membrane of the pollen vegetative cell and is composed mainly of pectin, cellulose, hemicellulose, hydrolytic enzymes, and hydrophobic proteins (Scott et al., 2004). Both the exine layer and the pollen coat layer are basically of a lipidic nature. The exine is largely formed from acyl lipid and phenylpropanoid precursors, which together form the mixed stable biopolymer known as sporopollenin (Gui...

show abstract

DYT1 directly regulates the expression of TDF1 for tapetum development and pollen wall formation in Arabidopsis

Cited by 131 publications

References 57 publications

Feedback Regulation of DYT1 by Interactions with Downstream bHLH Factors Promotes DYT1 Nuclear Localization and Anther Development

Feedback Regulation of DYT1 by Interactions with Downstream bHLH Factors Promotes DYT1 Nuclear Localization and Anther Development

IRREGULAR POLLEN EXINE1 Is a Novel Factor in Anther Cuticle and Pollen Exine Formation

Cytological and Transcriptomic Analyses Reveal Important Roles of CLE19 in Pollen Exine Formation

Contact Info

Product

Resources

About