<scp>B</scp>iocompatibility of ferroelectric lithium niobate and the influence of polarization charge on osteoblast proliferation and function

Carville, N. Craig; Collins, Liam; Manzo, Michele; Gallo, Katia; Lukasz, Bartlomiej; McKayed, Katey K.; Simpson, Jeremy C.; Rodriguez, Brian J.

doi:10.1002/jbm.a.35390

Cited by 58 publications

(55 citation statements)

References 64 publications

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“…The proliferation rate looks similar on the faces of LN and slightly higher than that on glass in the first 24 h, while negligible differences are observed after 48 h for all of the substrates. In fact, the population increases by ∼90% on LN faces and by ∼40% on glass, showing around 50% faster proliferation on LN, compared to glass in the first 24 h. This is in good agreement with the results reported by Carville et al, 31 who found significant differences in osteoblast cell proliferation between c+ and c− were observed after incubation for only 6 days.…”

Section: ■ Results and Discussionsupporting

confidence: 92%

Effects of Lithium Niobate Polarization on Cell Adhesion and Morphology

Marchesano

Gennari

Mecozzi

et al. 2015

ACS Appl. Mater. Interfaces

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Understanding how the interfacial effects influence cell adhesion and morphology is of fundamental interest for controlling function, growth, and movement of cells in vitro and in vivo. In particular, the influence of surface charges is well-known but still controversial, especially when new functional materials and methods are introduced. Here, the influence of the spontaneous polarization of ferroelectric lithium niobate (LN) on the adhesion properties of fibroblast cells is investigated. The spontaneous polarization of LN has one of the largest known magnitudes at room temperature (∼78 μC/cm(2)), and its orientation can be patterned easily by an external voltage, this motivating highly the investigation of its interaction with cells. Immunofluorescence and migration assays show strong evidence that the surface polarity regulates the adhesion functions, with enhanced spreading of the cytoskeleton on the negative face. The results suggest the potential of LN as a platform for investigating the role of charges on cellular processes, thus favoring new strategies in fabricating those biocompatible constructs used for tissue engineering. In fact, the orientation of the high-magnitude polarization can be patterned easily and, in combination with piezoelectric, pyroelectric, and photorefractive properties, may open the route to more sophisticated charge templates for modulating the cell response.

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Section: ■ Results and Discussionsupporting

confidence: 92%

Effects of Lithium Niobate Polarization on Cell Adhesion and Morphology

Marchesano

Gennari

Mecozzi

et al. 2015

ACS Appl. Mater. Interfaces

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“…19) and PPLT. 29 The results point to promising research directions exploiting LiNbO 3 as a neural substrate.…”

Section: à2mentioning

confidence: 99%

“…Alternatively, topographical features can be created in monodomain substrates via photo-or electron beam lithography. Recent work showed that uncoated LiNbO 3 surfaces are biocompatible with celltype specific effects: osteoblasts 19 and mesenchymal stem cells 20 exhibited increased proliferation and differentiation on charged Published by AIP Publishing. 110, 053702-1 charge was also shown to affect cell morphology and motility.…”

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confidence: 99%

“…Interestingly, the direction of the charge (i.e., negative or positive) did not affect the outcome, similar to the effects of charged LiNbO 3 on osteoblast proliferation and mineral accumulation. 19 Figure 2 shows neurons cultured on charge-patterned PPLN substrates. On these substrates, axons appeared to recognize and align with domain boundaries, reminiscent of the avoidance of domain boundaries by fibroblasts on a periodically poled lithium tantalate (PPLT).…”

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confidence: 99%

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Charge and topography patterned lithium niobate provides physical cues to fluidically isolated cortical axons

Kilinc

Blasiak

Baghban

et al. 2017

Applied Physics Letters

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In vitro devices that combine chemotactic and physical cues are needed for understanding how cells integrate different stimuli. We explored the suitability of lithium niobate (LiNbO3), a transparent ferroelectric material that can be patterned with electrical charge domains and micro/nanotopography, as a neural substrate. On flat LiNbO3 z-surfaces with periodically alternating charge domains, cortical axons are partially aligned with domain boundaries. On submicron-deep etched trenches, neurites are aligned with the edges of the topographical features. Finally, we bonded a bicompartmental microfluidic chip to LiNbO3 surfaces patterned by etching, to create isolated axon microenvironments with predefined topographical cues. LiNbO3 is shown to be an emerging neuron culture substrate with tunable electrical and topographical properties that can be integrated with microfluidic devices, suitable for studying axon growth and guidance mechanisms under combined topographical/chemical stimuli.

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“…The ferroelectric properties of certain crystals such as Lithium Niobate have been exploited recently to investigate the role of charge on cell behavior, and show great promise. 52 Ferroelectric materials have a spontaneous polarization which can be switched by an externally applied electric field. Therefore modulating polarization domains of user-specified size and shape are possible and this combined with the possibility of optically transparent samples yields a substrate ideal for cell-charge experiments.…”

Section: Effect Of Substrate Stiffnessmentioning

confidence: 99%

Elucidating the molecular mechanisms underlying cellular response to biophysical cues using synthetic biology approaches

Denning

Roos

2016

Cell Adhesion & Migration

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The use of synthetic surfaces and materials to influence and study cell behavior has vastly progressed our understanding of the underlying molecular mechanisms involved in cellular response to physicochemical and biophysical cues. Reconstituting cytoskeletal proteins and interfacing them with a defined microenvironment has also garnered deep insight into the engineering mechanisms existing within the cell. This review presents recent experimental findings on the influence of several parameters of the extracellular environment on cell behavior and fate, such as substrate topography, stiffness, chemistry and charge. In addition, the use of synthetic environments to measure physical properties of the reconstituted cytoskeleton and their interaction with intracellular proteins such as molecular motors is discussed, which is relevant for understanding cell migration, division and structural integrity, as well as intracellular transport. Insight is provided regarding the next steps to be taken in this interdisciplinary field, in order to achieve the global aim of artificially directing cellular response.

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Biocompatibility of ferroelectric lithium niobate and the influence of polarization charge on osteoblast proliferation and function

Cited by 58 publications

References 64 publications

Effects of Lithium Niobate Polarization on Cell Adhesion and Morphology

Effects of Lithium Niobate Polarization on Cell Adhesion and Morphology

Charge and topography patterned lithium niobate provides physical cues to fluidically isolated cortical axons

Elucidating the molecular mechanisms underlying cellular response to biophysical cues using synthetic biology approaches

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