Scorpion Venom Causes Apoptosis by Increasing Reactive Oxygen Species and Cell Cycle Arrest in MDA-MB-231 and HCT-8 Cancer Cell Lines

Alasmari, Abdulrahman; Riyasdeen, Anvarbatcha; Islam, Mohammad Mahmudul

doi:10.1177/2156587217751796

Cited by 21 publications

(22 citation statements)

References 37 publications

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“…The results of our study showed that fraction B obtained from Conus textile was able to increase the level of ROS in CLL. These results are in agreement with the results of previous studies that have shown that crude venom of Conus textile and other animal venoms have the ability to increase the level of ROS (Al-Asmari et al, 2018;Salimi et al, 2020).…”

Section: Fraction B Increased Ros Generation In Cll Blymphocytessupporting

confidence: 93%

Cytotoxicity Studies of the Crude venom and Fractions of Persian Gulf Snail (Conus Textile) on Chronic Lymphocytic Leukemia and Normal Lymphocytes

Salimi

Salehian

Aboutorabi³

et al. 2021

Asian Pac J Cancer Prev

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Background: Marine animals have been considered by many researchers due to their various pharmacological effects. One group of marine animals that have been studied is cone snails. The conotoxin obtained from these marine animals has various therapeutic effects. Methods: This study was designed to investigate the apoptotic effects of crude venom of Conus textile and its fractions (A and B) on chronic lymphocytic leukemia (CLL) cells. Accordingly, parameters such as cell viability, reactive oxygen species (ROS) level, collapse in mitochondrial membrane potential (MMP), lysosomal membrane damage and caspase-3 activation were evaluated. Results: The results showed that the crude venom (50, 100 and 200 µg/ml) from Conus textile and its fraction B (50, 100 and 200 µg/ml) significantly reduced viability in CLL B-lymphocyte. In addition, exposure of CLL B-lymphocyte to fraction B (50, 100 and 200 µg/ml) was associated with an increase in the level of ROS, the collapse of the MMP, damage to the lysosomal membrane, and activation of caspase-3. Conclusion: According to results, it was concluded that fraction B from crude venom of Conus textile causes selective toxicity on CLL B-lymphocyte with almost no effect on a normal lymphocyte. Furthermore, this venom fraction could be a promising candidate for induction of apoptosis in patients with CLL through the mitochondrial pathway.

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Section: Fraction B Increased Ros Generation In Cll Blymphocytessupporting

confidence: 93%

Cytotoxicity Studies of the Crude venom and Fractions of Persian Gulf Snail (Conus Textile) on Chronic Lymphocytic Leukemia and Normal Lymphocytes

Salimi

Salehian

Aboutorabi³

et al. 2021

Asian Pac J Cancer Prev

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“…One of the mechanisms of death induced by scorpion venoms is via caspases, the beneficial pathways in cancer therapy, as seen in our results, corroborating with the studies of other researchers who challenged different tumor cell lines, including leukemic cells when treated with the venom of the scorpion Heterometrus bengalensis Koch [31], cervical cancer cells ( Rhopalurus junceus ) [28], and prostate cancer cells ( Androctonus amoreuxi ) (Akef et al 2017). These results were also seen by Al-Asmari et al [32], where venoms of the scorpions Androctonus crassicauda, Androctonus bicolor, and Leiurus quinquestriatus also induced a death via apoptosis. Therefore, based on these findings and other published studies, a conclusion can be drawn that scorpion venom possesses cytotoxic and apoptotic properties against cervical cancer cell lines.…”

Section: Discussionsupporting

confidence: 79%

Tityus serrulatusScorpion Venom Induces Apoptosis in Cervical Cancer Cell Lines

Bernardes-Oliveira

Farias

Gomes

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Cervical cancer (CC) is classified as the fourth most common type of cancer in women worldwide and remains a serious public health problem in many underdeveloped countries. Human papillomavirus (HPV), mainly types 16 and 18, has been established as a precursory etiologic agent for this type of cancer. Several therapeutic attempts have been studied and applied, aiming at its control. However, not only do classical treatments such as chemotherapies and radiotherapies target tumor cells, but also they cause damage to several healthy cells. For these reasons, the search for new biologically active chemotherapeutic components is of great importance. In this study, we investigated the effect of Tityus serrulatus scorpion venom (TsV) on CC lines. There are very few studies exploring venom of scorpions, and, to our knowledge, no study has been conducted using the venom of the scorpion TsV for treatment of cervical cancer lines. After challenge with TsV, the MTT assay demonstrated cytotoxic effect on HeLa line. Similarly, the cell death process in HeLa analyzed by flow cytometry suggests death via caspase, since the pan-caspase inhibitor z-VAD-fmk significantly reduced the apoptotic response to the treatment. These results suggest that venom of TsV can be a potential source for the isolation of effective antiproliferative and apoptotic molecules in the treatment of CC.

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“…Many studies reported some physiological effects of the Chinese scorpion BmK venom known for its antiepileptic, analgesic, and, recently, antitumor properties. The use of this Chinese scorpion BmK venom revealed apoptogenic, immunosuppressive, and antiproliferative properties and demonstrated the potential of the cocktail for cancer-related diseases (Zhao et al, 2011; Li et al, 2014; Al-Asmari et al, 2016, 2018; Guo et al, 2016; Liu et al, 2017). The antitumor effect of the BmK venom was later accredited to an analgesic peptide called BmK AGAP and others (Zhao et al, 2011; Guo et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Monitoring the Early Antiproliferative Effect of the Analgesic–Antitumor Peptide, BmK AGAP on Breast Cancer Using Intravoxel Incoherent Motion With a Reduced Distribution of Four b-Values

et al. 2019

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Background: The present study aimed to investigate the possibility of using intravoxel incoherent motion (IVIM) diffusion magnetic resonance imaging (MRI) to quantitatively assess the early therapeutic effect of the analgesic–antitumor peptide BmK AGAP on breast cancer and also evaluate the medical value of a reduced distribution of four b -values. Methods: IVIM diffusion MRI using 10 b -values and 4 b -values (0–1,000 s/mm 2 ) was performed at five different time points on BALB/c mice bearing xenograft breast tumors treated with BmK AGAP. Variability in Dslow , Dfast , PF , and ADC derived from the set of 10 b -values and 4 b -values was assessed to evaluate the antitumor effect of BmK AGAP on breast tumor. Results: The data showed that PF values significantly decreased in rBmK AGAP-treated mice on day 12 ( P = 0.044). PF displayed the greatest AUC but with a poor medical value (AUC = 0.65). The data showed no significant difference between IVIM measurements acquired from the two sets of b -values at different time points except in the PF on the day 3. The within-subject coefficients of variation were relatively higher in Dfast and PF . However, except for a case noticed on day 0 in PF measurements, the results indicated no statistically significant difference at various time points in the rBmK AGAP-treated or the untreated group ( P < 0.05). Conclusion: IVIM showed poor medical value in the early evaluation of the antiproliferative effect of rBmK AGAP in breast cancer, suggesting sensitivity in PF . A reduced distribution of four b-values may provide remarkable measurements but with a potential loss of accuracy in the perfusion-related parameter PF .

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Scorpion Venom Causes Apoptosis by Increasing Reactive Oxygen Species and Cell Cycle Arrest in MDA-MB-231 and HCT-8 Cancer Cell Lines

Cited by 21 publications

References 37 publications

Cytotoxicity Studies of the Crude venom and Fractions of Persian Gulf Snail (Conus Textile) on Chronic Lymphocytic Leukemia and Normal Lymphocytes

Cytotoxicity Studies of the Crude venom and Fractions of Persian Gulf Snail (Conus Textile) on Chronic Lymphocytic Leukemia and Normal Lymphocytes

Tityus serrulatusScorpion Venom Induces Apoptosis in Cervical Cancer Cell Lines

Monitoring the Early Antiproliferative Effect of the Analgesic–Antitumor Peptide, BmK AGAP on Breast Cancer Using Intravoxel Incoherent Motion With a Reduced Distribution of Four b-Values

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