SCL/Tal-1 transcription factor acts downstream of cloche to specify hematopoietic and vascular progenitors in zebrafish

Liao, Eric C.; Paw, Barry H.; Oates, Andrew C.; Pratt, Stephen J.; Postlethwait, John H.; Zon, Leonard I.

doi:10.1101/gad.12.5.621

Cited by 324 publications

(292 citation statements)

References 34 publications

(23 reference statements)

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“…4 The close developmental relation between endothelial and hematopoietic cells within this extraembryonic structure led to the hypothesis that both cell systems are derived from a common stem cell, the hemangioblast. 5,6 This concept has been supported by several mouse knockout models in which both endothelial and hematopoietic cells were found to be deficient. 7,8 In addition, hemangioblasts have been derived from embryonic stem cells in vitro.…”

mentioning

confidence: 67%

“…Recently it has been suggested that during embryonal life both hematopoietic and endothelial cells are derived from a common precursor cell, the hemagioblast. [5][6][7][8] Experimental studies provide evidence that such cells persist during postnatal life. It has been shown that endothelial cells can be derived from immature cells that have been purified from peripheral blood.…”

Section: Discussionmentioning

confidence: 99%

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Derivation of a new hematopoietic cell line with endothelial features from a patient with transformed myeloproliferative syndrome

Fiedler

Henke

Ergün

et al. 2000

Cancer

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mentioning

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Derivation of a new hematopoietic cell line with endothelial features from a patient with transformed myeloproliferative syndrome

Fiedler

Henke

Ergün

et al. 2000

Cancer

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“…The effect of a lack of endothelium was tested in vivo with endothelium-deficient clo m39 mutants (69). The clo m39 allele includes deletion of lyscardiolipin acyltransferase, which is required for hemangioblast differentiation to hematopoietic and endothelial lineages (42,44,45,70). Although ren mRNA was expressed in early stage clo mutants (Fig.…”

Section: F535 Mural Renin Cells In Developing Zebrafishmentioning

confidence: 99%

Renin expression in developing zebrafish is associated with angiogenesis and requires the Notch pathway and endothelium

Rider

Mullins

Verdon

et al. 2015

American Journal of Physiology-Renal Physiology

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Rider SA, Mullins LJ, Verdon RF, MacRae CA, Mullins JJ. Renin expression in developing zebrafish is associated with angiogenesis and requires the Notch pathway and endothelium. Am J Physiol Renal Physiol 309: F531-F539, 2015. First published July 22, 2015 doi:10.1152/ajprenal.00247.2015.-Although renin is a critical regulatory enzyme of the cardiovascular system, its roles in organogenesis and the establishment of cardiovascular homeostasis remain unclear. Mammalian renin-expressing cells are widespread in embryonic kidneys but are highly restricted, specialized endocrine cells in adults. With a functional pronephros, embryonic zebrafish are ideal for delineating the developmental functions of renin-expressing cells and the mechanisms governing renin transcription. Larval zebrafish renin expression originates in the mural cells of the juxtaglomerular anterior mesenteric artery and subsequently at extrarenal sites. The role of renin was determined by assessing responses to renin-angiotensin system blockade, salinity variation, and renal perfusion ablation. Renin expression did not respond to renal flow ablation but was modulated by inhibition of angiotensin-converting enzyme and altered salinity. Our data in larval fish are consistent with conservation of renin's physiological functions. Using transgenic renin reporter fish, with mindbomb and cloche mutants, we show that Notch signaling and the endothelium are essential for developmental renin expression. After inhibition of angiogenesis, renin-expressing cells precede angiogenic sprouts. Arising from separate lineages, but relying on mutual interplay with endothelial cells, renin-expressing cells are among the earliest mural cells observed in larval fish, performing both endocrine and paracrine functions. renin; zebrafish; notch; endothelium; angiogenesis RENIN is the rate-limiting enzyme of the renin-angiotensin system (RAS). Mammalian ANG II, the effector of the RAS, is principally involved in blood pressure homeostasis by regulation of vasomotor tone and Na ϩ retention. ANG II also regulates cell proliferation and angiogenesis (12,22). Pathological activation of the RAS is associated with cardiovascular diseases, including hypertension and heart failure, and is consequently a major target of therapeutic agents (11).Renin and its cognate cells are required for normal renal development (2), including angiogenesis of the renal vascular tree (59). In mice, ablation of the renin gene or renin-expressing cells leads to renal developmental abnormalities (55,71,79). The vasculature of mammalian embryonic kidneys have a transient but extensive coverage of mural renin-expressing cells (17,31,32,47,63); however, their function is unclear. Adult renin-expressing cells are restricted to the juxtaglomerular apparatus and play a vital role in ion homeostasis by secreting activated renin enzyme via cytoplasmic granules (17,31,36,63). Upon physiological challenge by RAS inhibition or low Na ϩ , prearteriolar vascular smooth muscle cells (VSMC) reestablish their embryonic re...

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“…30 The concept that SCL plays an important role in vasculogenesis is consistent with recent reports that SCL is required for normal yolk sac formation 31 and that SCL can partially rescue both vascular and haematopoietic defects in the zebrafish mutant, cloche. 32 The complex pattern of SCL expression raises important questions about the molecular mechanisms underlying lineage-restricted SCL expression in different cell types. We have therefore identified a number of regulatory elements responsible for lineage-specific expression of the murine SCL gene in erythroid and CD34 + myeloid cells.…”

Section: Introductionmentioning

confidence: 99%

Chromatin structure and transcriptional regulation of the stem cell leukaemia (SCL) gene in mast cells

Göttgens

McLaughlin

et al. 1999

Leukemia

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The stem cell leukaemia (SCL) gene is a member of the basic helix-loop-helix family of transcription factors and is essential for the development of all haematopoietic lineages. SCL is expressed in pluripotent haematopoietic stem cells and also following commitment to the erythroid, mast and megakaryocytic lineages. The mechanisms responsible for this pattern of expression are poorly understood, but are likely to illuminate the molecular basis for stem cell development and lineage commitment. Here we present the first description of the regulation of the SCL gene in mast cells. In this study we systematically analysed the chromatin structure of a 45 kb region of the murine SCL locus in mast cells. The pattern of DNase 1 and restriction endonuclease hypersensitive sites in mast cells was distinct from, but overlapped with, the pattern previously described in erythroid and primitive myeloid cells. Each potential regulatory element was tested using transient reporter assays to assess their functional significance in mast cells. These studies identified two potent enhancers, one of which was downstream of the SCL gene. Further characterisation of this 3Ј enhancer demonstrated that it required the presence of two distinct DNase 1 hypersensitive sites for full activity, and that it was capable of stimulating transcription from both promoter 1a and 1b. Since the 3Ј enhancer is active in both erythroid and mast cells, it will now be important to see whether it is independently activated in these lineages, or whether it is also active in haematopoietic stem cells.

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SCL/Tal-1 transcription factor acts downstream of cloche to specify hematopoietic and vascular progenitors in zebrafish

Cited by 324 publications

References 34 publications

Derivation of a new hematopoietic cell line with endothelial features from a patient with transformed myeloproliferative syndrome

Derivation of a new hematopoietic cell line with endothelial features from a patient with transformed myeloproliferative syndrome

Renin expression in developing zebrafish is associated with angiogenesis and requires the Notch pathway and endothelium

Chromatin structure and transcriptional regulation of the stem cell leukaemia (SCL) gene in mast cells

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