Sciatic nerve regeneration after traumatic injury using magnetic targeted adipose-derived mesenchymal stem cells

Soto, Paula Andrea Botero; Vence, Marianela; Piñero, Gonzalo; Coral, Diego Fernando; Usach, Vanina; Muraca, Diego; Cueto, Alicia; Roig, Anna; Raap, M. B. Fernández van; Setton-Avruj, Patrícia

doi:10.1016/j.actbio.2021.05.050

Cited by 31 publications

(27 citation statements)

References 63 publications

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“…The stem cells such as mesenchymal stem cells (BMSCs) can differentiate as nerve cells, epithelial cells, and a variety of tissue cells [ 5 ]. BMSC is a type of adult stem cells came from the multiple tissues and has high potential to differentiate into multiple cells.…”

Section: Introductionmentioning

confidence: 99%

GAP-43 Induces the Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells into Retinal Ganglial-Like Cells

Wang

Nie

2022

Computational and Mathematical Methods in Medicine

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Optic neuritis (ON) is a common neurological disease, and the transplant of retinal ganglion cells (RGCs) has been thought as a promising strategy for improving the injury of the optic nerve system. Bone mesenchymal stem cells (BMSCs) have the potential to differentiate into neural cells. Several studies have indicated that GAP-43 is related with the regeneration of nerve cells, while the effect of GAP-43 on inducing BMSC differentiation remains unclear. In this study, the BMSCs were separated from the rats and identified with flow cytometry assay. The GAP-43 expressed vectors were transfected into the BMSCs, and the biomarkers of RGCs such as PAX6, LHX2, and ATOH7 were used to observe by qRT-PCR. Moreover, the effect of GAP-43-induced BMSCs (G-BMSCs) on ON improvement was also verified with rat models, and the activity of MAPK pathway was measured with western blot. Here, it was found that GAP-43 could obviously promote the differentiation of BMSCs, and increased PAX6, LHX2, ATOH7, BRN3A, and BRN3B were observed in the process of cell differentiation. Moreover, it was also found that G-BMSCs significantly increased the abundances of NFL and NFM in G-BMSCs, and GAP-43 could also enhance the activity of MAPK pathways in BMSCs. Therefore, this study suggested that GAP-43 could induce the differentiation of bone marrow-derived mesenchymal stem cells into retinal ganglial cells.

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Section: Introductionmentioning

confidence: 99%

GAP-43 Induces the Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells into Retinal Ganglial-Like Cells

Wang

Nie

2022

Computational and Mathematical Methods in Medicine

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“…83 At the same time, it was determined that the synergy of magnetic field with IONPs (3−17 nm) internalized in ADSCs (5 pg/iron per cell) and its confirmation by VSM analyses could improve the nerve conduction/cell function through enhancing myelination up to 41.2%. 84 Since the distal latency depends on the state of nerve myelination, the reduction of almost 2-fold the distal latency in the ADSCs-IONP stimulated by magnetic field compared to that without magnetic field indicates the positive effect of the magnetic field in the regeneration of the myelin structure in the axon. In confirmation of the above findings, the results of immunohistochemistry show an increase in the expression of myelin basic protein and β3 tubulin, which indicates an increase in myelination by the magnetic field.…”

Section: Effect Of Physicochemicalmentioning

confidence: 98%

Exploring the Physicochemical, Electroactive, and Biodelivery Properties of Metal Nanoparticles on Peripheral Nerve Regeneration

Sharifi

Kamalabadi-Farahani

Salehi

et al. 2022

ACS Biomater. Sci. Eng.

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Despite the advances in the regeneration/rehabilitation field of damaged tissues, the functional recovery of peripheral nerves (PNs), especially in a long gap injury, is considered a great medical challenge. Recent progress in nanomedicine has provided great hope for PN regeneration through the strategy of controlling cell behavior by metal nanoparticles individually or loaded on scaffolds/conduits. Despite the confirmed toxicity of metal nanoparticles due to long-term accumulation in nontarget tissues, they play a role in the damaged PN regeneration based on the topography modification of scaffolds/conduits, enhancing neurotrophic factor secretion, the ion flow improvement, and the regulation of electrical signals.Determining the fate of neural progenitor cells would be a major achievement in PN regeneration, which seems to be achievable by metal nanoparticles through altering cell vital approaches and controlling their functions. Therefore, in this literature, an attempt was made to provide an overview of the effective activities of metal nanoparticles on the PN regeneration, until the vital clues of the PN regeneration and how they are changed by metal nanoparticles are revealed to the researcher.

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“…Moreover, IONPs and IONP-loaded cells allow the delivery of therapeutic biomolecules, such as neurotrophic factors, drugs, proteins, DNA and siRNA by specific NP functionalization [403][404][405][406][407]. Functionalized IONPs and IONP-loaded cells can be effectively enriched at the injury site by external magnet fields to efficiently promote neuronal repair or guide axonal growth [408][409][410][411][412][413][414][415]. Finally, the use of scaffolds made of various nanomaterials can serve as structural support, induce or mimic the formation of an ECM, inhibit glial differentiation, promote neuronal growth and control hemostasis.…”

Section: Pns and Cns Regenerationmentioning

confidence: 99%

Iron Oxide Nanoparticles in Regenerative Medicine and Tissue Engineering

2021

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In recent years, many promising nanotechnological approaches to biomedical research have been developed in order to increase implementation of regenerative medicine and tissue engineering in clinical practice. In the meantime, the use of nanomaterials for the regeneration of diseased or injured tissues is considered advantageous in most areas of medicine. In particular, for the treatment of cardiovascular, osteochondral and neurological defects, but also for the recovery of functions of other organs such as kidney, liver, pancreas, bladder, urethra and for wound healing, nanomaterials are increasingly being developed that serve as scaffolds, mimic the extracellular matrix and promote adhesion or differentiation of cells. This review focuses on the latest developments in regenerative medicine, in which iron oxide nanoparticles (IONPs) play a crucial role for tissue engineering and cell therapy. IONPs are not only enabling the use of non-invasive observation methods to monitor the therapy, but can also accelerate and enhance regeneration, either thanks to their inherent magnetic properties or by functionalization with bioactive or therapeutic compounds, such as drugs, enzymes and growth factors. In addition, the presence of magnetic fields can direct IONP-labeled cells specifically to the site of action or induce cell differentiation into a specific cell type through mechanotransduction.

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Sciatic nerve regeneration after traumatic injury using magnetic targeted adipose-derived mesenchymal stem cells

Cited by 31 publications

References 63 publications

GAP-43 Induces the Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells into Retinal Ganglial-Like Cells

GAP-43 Induces the Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells into Retinal Ganglial-Like Cells

Exploring the Physicochemical, Electroactive, and Biodelivery Properties of Metal Nanoparticles on Peripheral Nerve Regeneration

Iron Oxide Nanoparticles in Regenerative Medicine and Tissue Engineering

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