Schwann cell-matrix coated PCL-MWCNT multifunctional nanofibrous scaffolds for neural regeneration

Al‒Hadeethi, Yas; Nagarajan, Aishwarya; Hanuman, Srividya; Mohammed, Hiba; Vetekar, Aakanksha M.; Thakur, Goutam; Đình, Lê; Yao, Yin; Mkawi, E.M.; Hussein, Mahmoud A.; Agarwal, Vipul; Nune, Manasa

doi:10.1039/d2ra05368c

Cited by 14 publications

(15 citation statements)

References 52 publications

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“…Such disruption by filler particles also disrupts intrinsic movement of polymer chains and their organization within a scaffold which can lead to a reduction in the stretchability of the scaffolds as observed here. The observed reduction in elongation at break of polymer matrices (films, scaffolds) are in agreement with previously reported studies [ 41 , 53 – 56 ].…”

Section: Discussionsupporting

confidence: 93%

“…We have previously demonstrated that Schwann cells also secrete extracellular matrix which helped to provide biochemical cues to improve cell proliferation and adhesion on the PCL nanofibrous scaffolds [ 24 ]. In another work, we also confirmed that Schwann cell acellular matrix when coated on PCL and multiwalled carbon nanotube scaffolds showed a significant expression of peripheral myelin protein 22 (PMP22) gene [ 41 ]. Based on these results, we deduced that Schwann cell matrix in combination with PCL scaffolds could promote more functional neuronal properties such as myelination.…”

Section: Introductionmentioning

confidence: 71%

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Polycaprolactone/graphene oxide/acellular matrix nanofibrous scaffolds with antioxidant and promyelinating features for the treatment of peripheral demyelinating diseases

Nagarajan,

Rizwana,

Abraham

et al. 2023

J Mater Sci: Mater Med

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Peripheral demyelinating diseases entail damage to axons and Schwann cells in the peripheral nervous system. Because of poor prognosis and lack of a cure, this group of diseases has a global impact. The primary underlying cause of these diseases involves the inability of Schwann cells to remyelinate the damaged insulating myelin around axons, resulting in neuronal death over time. In the past decade, extensive research has been directed in the direction of Schwann cells focusing on their physiological and neuroprotective effects on the neurons in the peripheral nervous system. One cause of dysregulation in the remyelinating function of Schwann cells has been associated with oxidative stress. Tissue-engineered biodegradable scaffolds that can stimulate remyelination response in Schwann cells have been proposed as a potential treatment strategy for peripheral demyelinating diseases. However, strategies developed to date primarily focussed on either remyelination or oxidative stress in isolation. Here, we have developed a multifunctional nanofibrous scaffold with material and biochemical cues to tackle both remyelination and oxidative stress in one matrix. We developed a nanofibrous scaffold using polycaprolactone (PCL) as a foundation loaded with antioxidant graphene oxide (GO) and coated this bioscaffold with Schwann cell acellular matrix. In vitro studies revealed both antioxidant and remyelination properties of the developed bioscaffold. Based on the results, the developed multifunctional bioscaffold approach can be a promising biomaterial approach for treating demyelinating diseases. Graphical Abstract

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 71%

Polycaprolactone/graphene oxide/acellular matrix nanofibrous scaffolds with antioxidant and promyelinating features for the treatment of peripheral demyelinating diseases

Nagarajan,

Rizwana,

Abraham

et al. 2023

J Mater Sci: Mater Med

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“…Manasa Nune et al [ 45 ] have proved that self-assembling peptide nanostructures on aligned PLGA nanofibers can provide better cell–matrix communication and stimulate nerve remyelination. Other study indicates that uniaxially oriented fibers could guide migration of SCs but surface properties of scaffolds may be more influential than scaffold morphology in nerve regeneration [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Electrical aligned polyurethane nerve guidance conduit modulates macrophage polarization and facilitates immunoregulatory peripheral nerve regeneration

Sun,

Zhang,

Guo

et al. 2024

J Nanobiotechnol

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Biomaterials can modulate the local immune microenvironments to promote peripheral nerve regeneration. Inspired by the spatial orderly distribution and endogenous electric field of nerve fibers, we aimed to investigate the synergistic effects of electrical and topological cues on immune microenvironments of peripheral nerve regeneration. Nerve guidance conduits (NGCs) with aligned electrospun nanofibers were fabricated using a polyurethane copolymer containing a conductive aniline trimer and degradable L-lysine (PUAT). In vitro experiments showed that the aligned PUAT (A-PUAT) membranes promoted the recruitment of macrophages and induced their polarization towards the pro-healing M2 phenotype, which subsequently facilitated the migration and myelination of Schwann cells. Furthermore, NGCs fabricated from A-PUAT increased the proportion of pro-healing macrophages and improved peripheral nerve regeneration in a rat model of sciatic nerve injury. In conclusion, this study demonstrated the potential application of NGCs in peripheral nerve regeneration from an immunomodulatory perspective and revealed A-PUAT as a clinically-actionable strategy for peripheral nerve injury.

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“…The integration of 3D printing technology in tissue engineering has revolutionized the creation of functional and implantable tissue constructs. Additive manufacturing techniques are employed to fabricate biomaterial scaffolds with patient-specific geometries and porous networks that facilitate tissue growth. , Traditional methods involve fabricating the scaffolds first and then incorporating the cells, which can result in a non-uniform cell distribution. − However, the emerging field of bioprinting allows for the simultaneous printing of cells and biomaterials, enabling precise cell placement within the printed tissues . Bioprinting techniques can be categorized as vat polymerization, material extrusion, or material jetting. − Vat polymerization-based printing involves the fabrication of scaffolds, onto which cell seeding is usually carried out.…”

Section: Introductionmentioning

confidence: 99%

Dual Crosslinked Antioxidant Mixture of Poly(vinyl alcohol) and Cerium Oxide Nanoparticles as a Bioink for 3D Bioprinting

Rizwana,

Maslekar,

Chatterjee

et al. 2023

ACS Appl. Nano Mater.

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Three-dimensional (3D) bioprinting has made it possible to fabricate structures with intricate morphologies and architectures, which is considered difficult to do when using other conventional techniques like electrospinning. Although the 3D printing of thermoplastics has seen a huge boom in the past few years, it has been challenging to translate this technology to cellbased printing. A major limitation in bioprinting is the lack of inks that allow for the printing of 3D structures that meet the biological requirements of a specific organ or tissue. A bioink is a viscous polymer solution that cells are incorporated into before printing. Therefore, a bioink must have specific characteristics to ensure both good printability and biocompatibility. Despite the progress that has been made in bioprinting, achieving a balance between these two properties has been difficult. In this work, we developed a multimodal bioink that serves as both a cell carrier and a free radical scavenger for treating peripheral nerve injury. This bioink comprises poly(vinyl alcohol) (PVA) and cerium oxide nanoparticles (also called nanoceria (NC)) and was developed with a dual crosslinking method that utilizes citric acid and sodium hydroxide. By employing this dual crosslinking method, good printability of the bioink and shape fidelity of the bioprinted structure were achieved. Additionally, a cell viability study demonstrated that the cells remained compatible and viable even after they underwent the printing process. The combination of this PVA/NC bioink and the dual crosslinking method proved to be effective in enhancing printability and cell biocompatibility for extrusion-based bioprinting applications.

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Schwann cell-matrix coated PCL-MWCNT multifunctional nanofibrous scaffolds for neural regeneration

Abstract: Schwann cell-derived matrix coated scaffolds for peripheral nerve regeneration.

Cited by 14 publications

References 52 publications

Polycaprolactone/graphene oxide/acellular matrix nanofibrous scaffolds with antioxidant and promyelinating features for the treatment of peripheral demyelinating diseases

Polycaprolactone/graphene oxide/acellular matrix nanofibrous scaffolds with antioxidant and promyelinating features for the treatment of peripheral demyelinating diseases

Electrical aligned polyurethane nerve guidance conduit modulates macrophage polarization and facilitates immunoregulatory peripheral nerve regeneration

Dual Crosslinked Antioxidant Mixture of Poly(vinyl alcohol) and Cerium Oxide Nanoparticles as a Bioink for 3D Bioprinting

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