Schistosoma mansoni: The Immune Response against Cercarial Glycocalyx

Abstract

Schistosoma mansoni cercarial glycocalyx was separated and purified by Sephacryl-300 SR. It was found to stimulate the humoral immune response in mice injected with it. Antiglycoalyx antibodies raised in CD/1 mice were found to be cytotoxic to schistosomula in vitro. But conversely, no protective effect was demonstrated in vivo. Eosinophil-mediated cytotoxicity was found to have no effector function in the murine immune response against schistosomes. A monoclonal antiglycocalyx IgM was prepared during our study. It was found to have no cytotoxic effect on schistosomules in vitro. However, it was found to have an inhibitory activity blocking the cytotoxic effect of other antiglycocalyx isotypes in the immune mouse. The contradiction between the result of antiglycocalyx antibody-mediated cytotoxicity obtained in vivo and that obtained in vitro is in itself revealing and suggests that the effect is crucially dependent upon factors as yet poorly understood.

Curcuma longa extract nanoemulsion preparation and demonstration of its in vitro activity against Schistosoma mansoni cercariae, schistosomules and adult stages

“…The organisms obtained were concentrated simply by gravity sedimentation on ice. The sediment was re-suspended in RPMI-1640 medium containing 10% heat-inactivated fetal calf serum, 100 IU/ ml penicillin and 100 µg/ml streptomycin [28] . About 20-40 schistosomules were added to each well of sterile tissue culture plates with flat-bottomed wells and incubated overnight in 5% CO2, 100% humidity, at 37 o C. After 24 h of incubation, Cl nanoemulsion was added to the culture medium to give final concentrations of 12.5, 25, 50, and 100 µg/ml.…”

“…The percentage of damaged schistosomules was measured by addition of trypan blue at a final dilution of 0.2%. Dark blue schistosomules that permitted penetration of the dye were considered damaged [28] .…”