Curcuma longa extract nanoemulsion preparation and demonstration of its in vitro activity against Schistosoma mansoni cercariae, schistosomules and adult stages

Aly, Nagwa S. M.; Husseein, Atef; Emam, Hanan Tawfeek; Rashed, Gehan

doi:10.21608/puj.2017.4736

Cited by 6 publications

(7 citation statements)

References 35 publications

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“…However, curcumin has low bioavailability and poor water solubility. Thus, Aly et al [ 60 ] tried to increase its solubility and permeability through the cellular membrane by making a nanoemulsion of Curcuma longa extract (i.e., the curcumin plant source). The nanoemulsion showed an effect against adults of S. mansoni in vitro (especially males).…”

Section: Resultsmentioning

confidence: 99%

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Nanotechnological approaches in the treatment of schistosomiasis: an overview

Carvalho,

Sarcinelli,

Patrício

2024

Beilstein J. Nanotechnol.

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Schistosomiasis causes over 200,000 deaths annually. The current treatment option, praziquantel, presents limitations, including low bioavailability and resistance. In this context, nanoparticles have emerged as a promising option for improving schistosomiasis treatment. Several narrative reviews have been published on this topic. Unfortunately, the lack of clear methodologies presented in these reviews leads to the exclusion of many important studies without apparent justification. This integrative review aims to examine works published in this area with a precise and reproducible method. To achieve this, three databases (i.e., Pubmed, Web of Science, and Scopus) were searched from March 31, 2022, to March 31, 2023. The search results included only original research articles that used nanoparticles smaller than 1 µm in the treatment context. Additionally, a search was conducted in the references of the identified articles to retrieve works that could not be found solely using the original search formula. As a result, 65 articles that met the established criteria were identified. Inorganic and polymeric nanoparticles were the most prevalent nanosystems used. Gold was the primary material used to produce inorganic nanoparticles, while poly(lactic-co-glycolic acid) and chitosan were commonly used to produce polymeric nanoparticles. None of these identified works presented results in the clinical phase. Finally, based on our findings, the outlook appears favorable, as there is a significant diversity of new substances with schistosomicidal potential. However, financial efforts are required to advance these nanoformulations.

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Section: Resultsmentioning

confidence: 99%

“…However, in this work, the death of females was only possible in a high concentration of the nanoformulation (100 µg/mL). Every dosage tested was also effective against young forms (esquistosomules) [ 60 ].…”

Section: Resultsmentioning

confidence: 99%

Nanotechnological approaches in the treatment of schistosomiasis: an overview

Carvalho,

Sarcinelli,

Patrício

2024

Beilstein J. Nanotechnol.

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“…They showed that this compound significantly reduced parasite load and inflammation in mice with no toxicity [ 58 ]. Investigation of the anthelmintic effects of CUR-NE on Schistosoma mansoni cercariae, schistosomules and adult stages, revealed optimal activity against the adult stage with decreased motor activity of the worms [ 59 ]. The protoscolicidal effects of curcumin and nanocurcumin have been shown in several studies.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic effect of curcumin nanoemulsion on cystic echinococcosis in BALB/c mice: a computerized tomography (CT) scan and histopathologic study evaluation

Ghanimatdan,

Sadjjadi,

Mikaeili

et al. 2024

BMC Complement Med Ther

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Background This study aimed to determine the therapeutic efficacy of curcumin nanoemulsion (CUR-NE) in mice infected with Echinococcus granulosus sensu stricto protoscoleces. Methods Forty-two inbred BALB/c mice were divided into seven groups of six animals each. Six groups were inoculated intra-peritoneally with 1500 viable E. granulosus protoscoleces, followed for six months and used as infected groups. The infected groups were named as: CEI1 to CEI6 accordingly. The 7th group was not inoculated and was named cystic echinococcosis noninfected group (CENI7). CEI1 and CEI2 groups received 40 mg/kg/day and 20 mg/kg/day curcumin nanoemulsion (CUR-NE), respectively. CEI3 received nanoemulsion without curcumin (NE-no CUR), CEI4 received curcumin suspension (CUR-S) 40 mg/kg/day, CEI5 received albendazole 150 mg/kg/day and CEI6 received sterile phosphate-buffered saline (PBS). CENI7 group received CUR-NE 40 mg/kg/day. Drugs administration was started after six months post-inoculations of protoscoleces and continued for 60 days in all groups. The secondary CE cyst area was evaluated by computed tomography (CT) scan for each mouse before treatment and on the days 30 and 60 post-treatment. The CT scan measurement results were compared before and after treatment. After the euthanasia of the mice on the 60th day, the cyst area was also measured after autopsy and, the histopathological changes of the secondary cysts for each group were observed. The therapeutic efficacy of CUR-NE in infected groups was evaluated by two methods: CT scan and autopsied cyst measurements. Results Septal calcification in three groups of infected mice (CEI1, CEI2, and CEI4) was revealed by CT scan. The therapeutic efficacy of CUR-NE 40 mg/kg/day (CEI1 group) was 24.6 ± 26.89% by CT scan measurement and 55.16 ± 32.37% by autopsied cysts measurements. The extensive destructive effects of CUR-NE 40 mg/kg/day (CEI1 group) on the wall layers of secondary CE cysts were confirmed by histopathology. Conclusion The current study demonstrated a significant therapeutic effect of CUR-NE (40 mg/kg/day) on secondary CE cysts in BALB/c mice. An apparent septal calcification of several cysts revealed by CT scan and the destructive effect on CE cysts observed in histopathology are two critical key factors that suggest curcumin nanoemulsion could be a potential treatment for cystic echinococcosis.

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“…Thus, it is known that the use of Tween 80 (HLB = 15) is categorized as an O/W surfactant that has a higher solubility in the aqueous phase than in the organic phase (Sahlan et al 2018 ). It is recognized that Tween 80 contains a lipophilic polysorbate group facing the lipophilic core of the curcumin micelle and a hydrophilic polyoxyethylene group facing hydrophilic solvents (Aly et al 2017 ), making it suitable for use to encapsulate curcumin from Curcuma longa L. (CUR).…”

Section: Discussionmentioning

confidence: 99%

“…Turmeric has several active compounds, with an emphasis on curcuminoids, which include curcumin (77% of curcuminoids), demethoxycurcumin (18%), and bisdemethoxycurcumin (5%), among them, the most important is curcumin, which corresponds to about 3–4% of the plant (Aly et al 2017 ). This phytoconstituent has several therapeutic effects, such as, anti-inflammatory, antitumor, and antimicrobial action for several microorganisms, among them, gram-negative and gram-positive bacteria (Alves et al 2017 ; Karimi et al 2018 ; Reddy et al 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Olive oil nanoemulsion containing curcumin: antimicrobial agent against multidrug-resistant bacteria

Confessor,

Agreles,

Campos

et al. 2024

Appl Microbiol Biotechnol

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The present work aimed to develop, characterize, and evaluate the antibacterial and antibiofilm activity of two nanoemulsions (NEs) containing 500 µg/mL of curcumin from Curcuma longa (CUR). These NEs, produced with heating, contain olive oil (5%) and the surfactants tween 80 (5%) and span 80 (2.5%), water q.s. 100 mL, and were stable for 120 days. NE-2-CUR presented Ø of 165.40 ± 2.56 nm, PDI of 0.254, ζ of − 33.20 ± 1.35 mV, pH of 6.49, and Entrapment Drug Efficiency (EE) of 99%. The NE-4-CUR showed a Ø of 105.70 ± 4.13 nm, PDI of 0.459, ζ of − 32.10 ± 1.45 mV, pH of 6.40 and EE of 99.29%. Structural characterization was performed using DRX and FTIR, thermal characterization using DSC and TG, and morphological characterization using SEM, suggesting that there is no significant change in the CUR present in the NEs and that they remain stable. The MIC was performed by the broth microdilution method for nine gram-positive and gram-negative bacteria, as well as Klebsiella pneumoniae clinical isolates resistant to antibiotics and biofilm and efflux pump producers. The NEs mostly showed a bacteriostatic profile. The MIC varied between 125 and 250 µg/mL. The most sensitive bacteria were Staphylococcus aureus and Enterococcus faecalis, for which NE-2-CUR showed a MIC of 125 µg/mL. The NEs and ceftazidime (CAZ) interaction was also evaluated against the K. pneumoniae resistant clinical isolates using the Checkerboard method. NE-2-CUR and NE-4-CUR showed a synergistic or additive profile; there was a reduction in CAZ MICs between 256 times (K26-A2) and 2 times (K29-A2). Furthermore, the NEs inhibited these isolates biofilms formation. The NEs showed a MBIC ranging from 15.625 to 250 µg/mL. Thus, the NEs showed physicochemical characteristics suitable for future clinical trials, enhancing the CAZ antibacterial and antibiofilm activity, thus becoming a promising strategy for the treatment of bacterial infections caused by multidrug-resistant K. pneumoniae. Key points • The NEs showed physicochemical characteristics suitable for future clinical trials. • The NEs showed a synergistic/additive profile, when associated with ceftazidime. • The NEs inhibited biofilm formation of clinical isolates.

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Curcuma longa extract nanoemulsion preparation and demonstration of its in vitro activity against Schistosoma mansoni cercariae, schistosomules and adult stages

Cited by 6 publications

References 35 publications

Nanotechnological approaches in the treatment of schistosomiasis: an overview

Nanotechnological approaches in the treatment of schistosomiasis: an overview

Therapeutic effect of curcumin nanoemulsion on cystic echinococcosis in BALB/c mice: a computerized tomography (CT) scan and histopathologic study evaluation

Olive oil nanoemulsion containing curcumin: antimicrobial agent against multidrug-resistant bacteria

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