Schistosoma mansoni: Rapid turnover of glycogen by adult worms in vivo

Tielens, Aloysius G.M.; Verwijs, A.; Elfring, R. H.; Heuvel, J.M. van den; Bergh, S.G. van den

doi:10.1016/0014-4894(89)90103-3

Cited by 8 publications

(3 citation statements)

References 4 publications

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“…The work suggests that the substantial lactate generated by glycolysis in blood-dwelling schistosomes can be excreted across the parasite tegument via the tegumental membrane protein SmAQP. These data provide a molecular understanding of how schistosomes cope with the large quantities of lactate generated from the largely anaerobic carbohydrate metabolism that is a hallmark of their intravascular lives [4], [5], [8], [21], [39].…”

Section: Discussionmentioning

confidence: 94%

“…During transformation, the cercarial outer membrane is cast off and a new intravascular, double lipid bilayer covering is formed [2], [3]. The parasites also undergo a change in energy metabolism: free swimming cercariae metabolize glycogen stores via oxidative phosphorylation to CO 2 and H 2 O whereas intravascular life forms metabolize glycogen largely via glycolysis, with the generation of lactic acid as the major end product [4], [5].…”

Section: Introductionmentioning

confidence: 99%

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The Tegument of the Human Parasitic Worm Schistosoma mansoni as an Excretory Organ: The Surface Aquaporin SmAQP Is a Lactate Transporter

et al. 2010

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Adult schistosomes are intravascular parasites that metabolize imported glucose largely via glycolysis. How the parasites get rid of the large amounts of lactic acid this generates is unknown at the molecular level. Here, we report that worms whose aquaporin gene (SmAQP) has been suppressed using RNAi fail to rapidly acidify their culture medium and excrete less lactate compared to controls. Functional expression of SmAQP in Xenopus oocytes demonstrates that this protein can transport lactate following Michaelis-Menten kinetics with low apparent affinity (Km = 41±5. 8 mM) and with a low energy of activation (Ea = 7.18±0.7 kcal/mol). Phloretin, a known inhibitor of lactate release from schistosomes, also inhibits lactate movement in SmAQP-expressing oocytes. In keeping with the substrate promiscuity of other aquaporins, SmAQP is shown here to be also capable of transporting water, mannitol, fructose and alanine but not glucose. Using immunofluorescent and immuno-EM, we confirm that SmAQP is localized in the tegument of adult worms. These findings extend the proposed functions of the schistosome tegument beyond its known capacity as an organ of nutrient uptake to include a role in metabolic waste excretion.

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Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

The Tegument of the Human Parasitic Worm Schistosoma mansoni as an Excretory Organ: The Surface Aquaporin SmAQP Is a Lactate Transporter

et al. 2010

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“…Heterologous expression of SmAQP in Xenopus oocytes demonstrated that this protein, in addition to transporting water, could also transport lactate [80]. These findings provide a molecular understanding of how schistosomes cope with the significant quantities of lactate created from the largely anaerobic glucose catabolism that is a hallmark of their intravascular lives[81]. Collectively, the comparative- and functional- genomic data suggest that the syntenic position of aquaporins is an important feature of schistosome/host relationships driven by the need to extend tegumental functions in metabolic waste excretion.…”

Section: Functionally Exploring Comparative Genomic Outputs: Revisitimentioning

confidence: 99%

Schistosoma comparative genomics: integrating genome structure, parasite biology and anthelmintic discovery

Swain

Larkin

Caffrey

et al. 2011

Trends in Parasitology

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Schistosoma genomes provide a comprehensive resource for identifying the molecular processes that shape parasite evolution and for discovering novel chemotherapeutic or immunoprophylactic targets. Here, we demonstrate how intra- and intergenus comparative genomics can be used to drive these investigations forward, illustrate the advantages and limitations of these approaches and review how post genomic technologies offer complementary strategies for genome characterisation. While sequencing and functional characterisation of other schistosome/platyhelminth genomes continues to expedite anthelmintic discovery, we contend that future priorities should equally focus on improving assembly quality, and chromosomal assignment, of existing schistosome/platyhelminth genomes.

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Schistosoma mansoni: Biochemical characteristics of the antischistosomal effects of Ro 15-5458

Eshete

Bennett

1990

Experimental Parasitology

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Schistosoma mansoni: Rapid turnover of glycogen by adult worms in vivo

Cited by 8 publications

References 4 publications

The Tegument of the Human Parasitic Worm Schistosoma mansoni as an Excretory Organ: The Surface Aquaporin SmAQP Is a Lactate Transporter

The Tegument of the Human Parasitic Worm Schistosoma mansoni as an Excretory Organ: The Surface Aquaporin SmAQP Is a Lactate Transporter

Schistosoma comparative genomics: integrating genome structure, parasite biology and anthelmintic discovery

Schistosoma mansoni: Biochemical characteristics of the antischistosomal effects of Ro 15-5458

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