Schistosoma mansoni: In vitro schistosomicidal activity of piplartine

Moraes, Josué de; Nascimento, Carlos; Lopes, Priscilla O.M.V.; Nakano, Eliana; Yamaguchi, Lydia F.; Kato, Massuo Jorge; Kawano, Toshie

doi:10.1016/j.exppara.2010.08.021

Cited by 137 publications

(153 citation statements)

References 40 publications

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“…Disparities in drug susceptibility between male and female S. mansoni worms have been previously reported in several in vitro activity trials (34) . The results of the present study showed that the susceptibility of male and female worms to venom varied, with male worms being more susceptible than females were.…”

Section: Discussionmentioning

confidence: 79%

“…In this regard, male S. mansoni worms were also more susceptible than females were to praziquantel, ginger extract, diamines, and amino alcohols and to treatment with certain essential oils (35) (36) (37) (38) (39) . However, there was no differential sensitivity to piplartine between male and female worms in vitro (34) . In contrast, treatment with other compounds such as amino alkanethiosulfuric acids (40) , 2-(butylamino)-1-phenyl-1-ethanethiosulfuric acid (41) , and artesunate (42) produced higher mortality rates among female S. mansoni worms than among males.…”

Section: Discussionmentioning

confidence: 99%

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In vitro antischistosomal activity of venom from the Egyptian snake Cerastes cerastes

Hassan

Abdel-Rahman

Ibrahim

et al. 2016

Rev. Soc. Bras. Med. Trop.

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Introduction:We studied the potential in vitro antischistosomal activity of Cerastes cerastes venom on adult Schistosoma mansoni worms. Methods: Live specimens of the horned viper snake, C. cerastes were collected from the Aswan Governorate (Egypt). Venom was collected from snakes by manual milking. Worms of S. mansoni were obtained from infected hamsters by perfusion and isolated from blood using phosphate buffer. Mortality rates of worms were monitored after 3 days of exposure to snake venom at LC 50 and various sublethal concentrations (10, 5, 2.5µg/ml). Scanning electron microscopy was used to investigate tegumental changes in treated worms after exposure to LC 50 doses of venom. Results: The LC 50 of C. cerastes venom was 21.5µg/ml. The effect of C. cerastes venom on Schistosoma worms varied according to their sex. The mortality rate of male and female worms after 48-h exposure was 83.3% and 50% , respectively. LC 50 of C. cerastes venom induced mild to severe tegumental damage in Schistosoma worms in the form of destruction of the oral sucker, shrinkage and erosion of the tegument, and loss of some tubercle spines. Conclusions: The present study demonstrated that C. cerastes venom exerts potential in vitro antischistosomal activity in a time and dose-dependent manner. These results may warrant further investigations to develop novel schistosomicidal agents from C. cerastes snake venom.

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

In vitro antischistosomal activity of venom from the Egyptian snake Cerastes cerastes

Hassan

Abdel-Rahman

Ibrahim

et al. 2016

Rev. Soc. Bras. Med. Trop.

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“…H erein, concerni ng t he teg ument al changes wit h curcumi n, t he adult w orms s how ed a v ariable degree of teg ument al changes i n t he f orm of retract ed and comple te d estruc tion in the oral and v entral s uck ers i n bot h fem ale and mal e worms. Moraes et al (2011) rep orted that piplartine induc ed morphologic al chang es in the or al and v entr al suck er regions of the worms. de Oliveira et al ( 2012) noticed changes in t he s uckers (or al and v entral) aft er in vitro exp osur e to ess ential oil of B accharis trimera over an i ncub ation p eriod of 24 hours.…”

Section: Discussionmentioning

confidence: 99%

ULTRASTRUCTURAL STUDY ON THE TEGUMENTAL ALTERATIONS OF MALE AND FEMALE SHISTOSOMA MANSONI Following IN VITRO EXPOSURE TO CURCUMIN

Mira¹

2017

Egypt. J. Exp. Biol. (Zoo.)

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N ab i l a M. Mi r aUltrastructural study on the tegu mental alteration s of adult m ale and female Schistos oma mans oni follo wing in v itro expo su re to curcumin ABSTRACT: Schis tos omes hav e plagued hum ans f or at least four millennia. S ucc ess of i nfec tion is mainl y at tributed to its unique double membrane out er -surf ace or t egument , whic h regulat es nutritional and immunol ogical inter actions between t he p arasit e and the hos t. T his s tud y has been und ertak en t o eval uate t he sc hist os omicidal activit y of curcumi n on t he ultr astr uct ure of t egum ent of adult blood fluk e, S. mans oni . W hole fl uk es were expos ed in vitro to c urc umin f or 3 and 6 hours at conc entrati ons of 10, 50, and 100 µM/ml and t egument al c hanges wer e observed b y scanning electr on microsc opy. Result s obt ained rev eal ed that c urcumi n treated fem ale and male worms lost t heir norm al s urfac e arc hitect ure, sinc e t heir surface s howed destr ucti on i n t he oral and ventral s uck ers as w ell as parti al loss of tubercles' spines , erosi on in int er -tubercl e teg ment al regions and appearance of num erous small blebs ar ound tubercl es wer e deformed. The p erceiv ed def ormati ons wer e found to be tim e and c oncentration dependent. T hese findings confirmed t hat curcumi n could be c onsider ed as a promising therapeutic agent with antisc hist osom al activit y and it may repres ent a promising alter native tr eatm ent f or the contr ol of S . mans oni. KEY WORDS:Schis tos oma m ans oni, curc umin, t egum ent , ultrastr uct ure, S E M.

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“…It has been suggested that, because of the large number of constituents, essential oils may have no specific cellular target 31 . Additionally, as the lipophilic compounds present in essential oils can pass through the cell wall, integument, and cytoplasmic membrane, they may damage the structure of the cellular membranes during their passage, which may cause cellular lysis 32,33 .…”

Section: Groups Incubation Period (H) Number Of Dead Worms (%) Changementioning

confidence: 99%

Ultrastructural study of morphological changes in Schistosoma mansoni after in vitro exposure to the monoterpene rotundifolone

Rocha

Cavalcanti

Barbosa-Filho

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Introduction: Schistosomiasis, a parasitic disease caused by trematode flatworms of the genus Schistosoma, affects more than 200 million people worldwide, and its control is dependent on a single drug, praziquantel. Here, we report the in vitro effect of rotundifolone, a monoterpene isolated from Mentha x villosa (Lamiaceae), on Schistosoma mansoni adult worms. Methods: The in vitro effect of rotundifolone on adult Schistosoma mansoni was evaluated by analysis of behavior and mortality and through a scanning electron microscopic analysis of ultrastructural changes in the tegument of the worms. Results: At concentrations of 3.54 and 7.09μg/mL -1 rotundifolone, no worm mortality was observed at any of the sampling intervals. A minor reduction in movement of the tail, suckers, and gynecophoral canal membrane was observed after 96 h of exposure to 7.09μg/mL -1 rotundifolone. At 70.96μg/mL -1 , a lack of movement was observed from 72h onwards and all worms were deemed dead; similar effects were observed at 48h with 177.4μg/mL -1 , and at 24h with 354.8μg/mL -1 and 700.96μg/mL -1 . Rotundifolone also caused death of all parasites and separation of coupled pairs into individual males and females after 24h at 354.8μg/mL -1 . Conclusions: The main changes in the tegument induced by the different ROT treatments were: after 24h incubation, bubble lesions spread over the entire body and loss of tubercles occurred in some regions of the ventral region.

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Schistosoma mansoni: In vitro schistosomicidal activity of piplartine

Cited by 137 publications

References 40 publications

In vitro antischistosomal activity of venom from the Egyptian snake Cerastes cerastes

In vitro antischistosomal activity of venom from the Egyptian snake Cerastes cerastes

ULTRASTRUCTURAL STUDY ON THE TEGUMENTAL ALTERATIONS OF MALE AND FEMALE SHISTOSOMA MANSONI Following IN VITRO EXPOSURE TO CURCUMIN

Ultrastructural study of morphological changes in Schistosoma mansoni after in vitro exposure to the monoterpene rotundifolone

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