Schistosoma mansoni: Identification and Protective Immunity of Adult Worm Antigens Recognized by T Lymphocytes of Outbred Swiss Mice Immunized with Irradiated Cercariae

Ridi, Rashika El; Tawab, Nagwa Abdel; Guirguis, N.

doi:10.1006/expr.1993.1032

Cited by 19 publications

(13 citation statements)

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“…SG3PDH shows 72% identity at the amino acid level with the human enzyme (22) and thus may have limited non-self T-cell epitopes capable of inducing immune responses in humans. The low number of Tcell epitopes on SG3PDH (2) and the well-characterized genetic restriction of mammalian hosts to protein T-cell epitopes (17,22) may explain why a substantial proportion of humans do not recognize this molecule.…”

Section: Discussionmentioning

confidence: 99%

Human T- and B-Cell Responses toSchistosoma mansoniRecombinant Glyceraldehyde 3-Phosphate Dehydrogenase Correlate with Resistance to Reinfection withS. mansoniorSchistosoma haematobiumafter Chemotherapy

et al. 2001

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Recently we reported that human T-and B-cell recognition of a 42-kDa protein (p42) in soluble extracts of adult Schistosoma mansoni worms correlates with resistance to reinfection with S. mansoni or S. haematobium. Amino acid microsequencing of p42 revealed that it consists predominantly of schistosome glyceraldehyde 3-phosphate dehydrogenase (SG3PDH). We have expressed SG3PDH in Escherichia coli and purified the recombinant protein in a soluble and enzymatically active form. Recombinant SG3PDH (rSG3PDH) reacted with human monospecific antibodies to p42. Lymphoproliferation and production of interleukin-4 and gamma interferon (IFN-␥) after in vitro stimulation with rSG3PDH and serum isotype responses to rSG3PDH were examined in individuals with extremes of resistance and susceptibility to reinfection after treatment of previous S. mansoni or S. haematobium infection. Lymphoproliferation and IFN-␥ production in response to rSG3PDH and the presence of serum immunoglobulin G1 (IgG1), IgG3, and IgA antibodies to rSG3PDH generally characterized individuals who are resistant to reinfection after chemotherapy. The data indicate that T-and B-cell immune reactivity to rSG3PDH correlates with resistance to reinfection, confirming previous studies identifying SG3PDH as a target of protective immunity in humans, and suggest that SG3PDH should be investigated as a possible vaccine for human schistosomiasis.

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Section: Discussionmentioning

confidence: 99%

Human T- and B-Cell Responses toSchistosoma mansoniRecombinant Glyceraldehyde 3-Phosphate Dehydrogenase Correlate with Resistance to Reinfection withS. mansoniorSchistosoma haematobiumafter Chemotherapy

et al. 2001

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“…Soluble antigens from S. mansoni adult worms (SAWA) were prepared as described (El Ridi et al 1993). In indirect ELISA, wells of polystyrene plates (Costar, Cambridge, MA, USA) were coated with 500 ng soluble schistosome antigens, blocked with 1% bovine serum albumin (BSA) in 0.05 M phosphate buffered saline (PBS) pH 7.1, washed with PBS/0.05% Tween 20 (washing buffer), and incubated with 100 ml serum diluted in washing buffer.…”

Section: Evaluation Of Humoral Factorsmentioning

confidence: 99%

Congenital exposure to Schistosoma mansoni infection: Impact on the future immune response and the disease outcome

et al. 2010

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“…Such immune sera have been used to identify and characterize potential vaccine antigens (Dalton et al, 1986(Dalton et al, , 1987Xu et al, 1989;Soisson et al, 1992). Hence, studies were conducted in several laboratories in an effort to identify the S. mansoni soluble adult worm antigen (SAWA) bands recognized by T lymphocytes of outbred Swiss (El Ridi et al, 1993) and inbred C57BL/6 (Reynolds et al, 1990;Mountford and Wilson, 1993;Richter et al, 1993;Osman et al, 1994aOsman et al, , 1994b and CBA (Richter et al, 1993) mice immunized twice with radiation-attenuated cercariae of S. mansoni. Hence, studies were conducted in several laboratories in an effort to identify the S. mansoni soluble adult worm antigen (SAWA) bands recognized by T lymphocytes of outbred Swiss (El Ridi et al, 1993) and inbred C57BL/6 (Reynolds et al, 1990;Mountford and Wilson, 1993;Richter et al, 1993;Osman et al, 1994aOsman et al, , 1994b and CBA (Richter et al, 1993) mice immunized twice with radiation-attenuated cercariae of S. mansoni.…”

mentioning

confidence: 99%

A Schistosoma mansoni 62-KDA Band Is Identified as an Irradiated Vaccine T-Cell Antigen and Characterized as Calreticulin

Gengehi¹,

Ridi²,

Tawab³

et al. 2000

The Journal of Parasitology

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The Schistosoma mansoni soluble adult worm antigen (SAWA) bands of 62/60 kDa were found to contain immunodominant T-cell immunogen(s) in irradiated cercariae-immunized Swiss and C57BL/6 mice. In the present study, spleen T cells of BALB/c mice immunized twice with ultraviolet light-irradiated cercariae proliferated and produced interleukin 4 in response to the 62/60-kDa SAWA bands in T-cell western assays. To characterize the 62/60-kDa bands, an adult S. mansoni worm cDNA expression library constructed in lambdagt11 was immunoscreened with serum of mice immunized with the 62/60-kDa antigens, and the immunoreactive cDNA inserts were sequenced. Purified 62- and 60-kDa proteins were used for amino acid microsequencing and for immunization studies in Swiss, C57BL/6, and BALB/c mice and rabbits. Taken together, the data indicated that the 60-kDa molecules are poorly immunogenic in mice and rabbits, whereas the 62-kDa species identified as S. mansoni calreticulin, is a good T- and B-cell antigen and represents a potential vaccine candidate.

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Schistosoma mansoni: Identification and Protective Immunity of Adult Worm Antigens Recognized by T Lymphocytes of Outbred Swiss Mice Immunized with Irradiated Cercariae

Cited by 19 publications

References 0 publications

Human T- and B-Cell Responses toSchistosoma mansoniRecombinant Glyceraldehyde 3-Phosphate Dehydrogenase Correlate with Resistance to Reinfection withS. mansoniorSchistosoma haematobiumafter Chemotherapy

Human T- and B-Cell Responses toSchistosoma mansoniRecombinant Glyceraldehyde 3-Phosphate Dehydrogenase Correlate with Resistance to Reinfection withS. mansoniorSchistosoma haematobiumafter Chemotherapy

Congenital exposure to Schistosoma mansoni infection: Impact on the future immune response and the disease outcome

A Schistosoma mansoni 62-KDA Band Is Identified as an Irradiated Vaccine T-Cell Antigen and Characterized as Calreticulin

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