Schistosoma mansoni antigens alter the cytokine response in vitro during cutaneous leishmaniasis

Bafica, Aline Michelle Barbosa; Cardoso, Luciana Santos; Oliveira, Sérgio C.; Loukas, Alex; Varela, Giuseppe Tittoni; Oliveira, Ricardo Riccio; Bacellar, Olı́via; Carvalho, Edgar M.; Araújo, Maria Ilma

doi:10.1590/s0074-02762011000700012

Cited by 13 publications

(22 citation statements)

References 36 publications

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“…Moreover, the downmodulation of IFN-γ production by rSm29 and rShTSP-2 antigens was followed by an increase in IL-10 production in the majority of individuals, and this augment in IL-10 production reached more than 300%. Reduction of IFN-γ production and an increase in the levels of IL-10 by the use of rSm29 and PIII antigens in PBMC cultures was also seen by our research team in patients with cutaneous leishmaniasis, the pathology of which is also linked to a Th1-immune response [20]. …”

Section: Discussionmentioning

confidence: 82%

“…Unexpectedly, we did not observe any other difference between the group of individuals who had reduction in the levels of IFN-γ and those who did not. In patients with cutaneous leishmaniasis [20], the addition of Sm29 and PIII to cell cultures stimulated with the Leishmania- soluble antigen resulted in a reduction of IFN-γ production, also with no correlation to the clinical features of the disease.…”

Section: Discussionmentioning

confidence: 99%

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Schistosoma Antigens Downmodulate the in vitro Inflammatory Response in Individuals Infected with Human T Cell Lymphotropic Virus Type 1

Lima

Santos

Oliveira

et al. 2013

Neuroimmunomodulation

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Human T cell lymphotropic virus type 1 (HTLV-1) is the causal agent of HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). While the immune response to HTLV-1 infection is polarized to the Th1-type, chronic helminth infections drive the Th2- and T regulatory-type, and are able to downregulate the inflammatory response in some autoimmune diseases. Objective: To evaluate whether Schistosoma spp. antigens alter the in vitro cytokine response in HTLV-1 infection. Methods: The recombinant Schistosoma antigens Sm29 and ShTSP2 (tetraspanin) and PIII, a fraction of the Schistosoma mansoni adult worm antigen were added to peripheral blood mononuclear cell (PBMC) cultures of HTLV-1-infected individuals and the levels of interferon (IFN)-γ and interleukin (IL)-10 in the supernatants were measured using the ELISA sandwich technique. Results: Compared to the levels of cytokine in nonstimulated cultures, the levels of IFN-γ were reduced in 50, 47 and 50% of patients by the presence of Sm29, ShTsp2 and PIII, respectively. The downregulation of IFN-γ production in the presence of Sm29 antigen was observed mainly in subjects who had lower basal levels of this cytokine. The levels of IL-10, however, increased by the addition of the three antigens in the cultures in 74, 62 and 44% of individuals, respectively. In addition, there was a decrease in the ratio of IFN-γ/IL-10 levels in cultures stimulated with Sm29 and ShTSP2 when compared to nonstimulated ones. Conclusions: The Schistosoma spp. antigens used in this study were able to downmodulate IFN-γ production in vitro in HTLV-1 infection. This may be associated with the increased levels of IL-10 induced by the antigens.

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Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Schistosoma Antigens Downmodulate the in vitro Inflammatory Response in Individuals Infected with Human T Cell Lymphotropic Virus Type 1

Lima

Santos

Oliveira

et al. 2013

Neuroimmunomodulation

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“…Data from our group showed that cultured PBMCs from leishmaniasis patients after being stimulated with Schistosoma mansoni antigens were able to increase IL-10 production and decrease TNF and IFN-γ production (Bafica et al, 2011). IL-10 derived from DCs may limit Th1 expansion through the inhibition of IL-12 itself (Corinti et al, 2001) or through its ability to stimulate IL-10 production from T cells (McGuirk et al, 2002), thus reducing the pro-duction of TNF and IFN-γ.…”

Section: Discussionmentioning

confidence: 99%

“…In cutaneous leishmaniasis the predominant immune response is the Th1/inflammatory type that is associated with parasitic elimination, but is also responsible for the development of the characteristic lesion observed in the disease (Ribeiro-de-Jesus et al, 1998; Antonelli et al, 2005). Studies have demonstrated that helminth infection has the ability to modulate the immune response in immune-mediated diseases, such as asthma (Medeiros et al, 2003; Araujo et al, 2004a,b), Crohn’s disease (Elliott et al, 2007), type 1 diabetes mellitus (Cooke et al, 1999), HTLV infection (Porto et al, 2005; Lima et al, 2013), and leishmaniasis (O’Neal et al, 2007; Bafica et al, 2011). It has been shown that infection by Schistosoma mansoni or its products are able to modulate the Th1 inflammatory response (Actor et al, 1993; Sabin et al, 1996) involved in some immune-mediated diseases.…”

Section: Introductionmentioning

confidence: 99%

Susceptibility of dendritic cells from individuals with schistosomiasis to infection by Leishmania braziliensis

Lopes

Almeida

Souza

et al. 2018

Molecular Immunology

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Coinfection with leishmaniasis and schistosomiasis has been associated with increased time to healing of cutaneous lesions of leishmaniasis. The objective of this study was to evaluate the effect of Leishmania braziliensis infection on co-cultures of monocyte-derived dendritic cells (MoDCs) with autologous lymphocytes from patients with schistosomiasis and patients with cutaneous leishmaniasis. MoDCs were differentiated from peripheral blood monocytes, isolated by magnetic beads, infected with L. braziliensis, and co-cultured with autologous lymphocytes. Expression of HLA-DR, CD1a, CD83, CD80, CD86, CD40, and the IL-10 receptor (IL-10R) on MoDCs as well as CD28, CD40L, CD25, and CTLA-4 on lymphocytes were evaluated by flow cytometry. The production of the cytokines IL-10, TNF, IL-12p40, and IFN-γ were evaluated by sandwich ELISA of the culture supernatant. The infectivity evaluation was performed by light microscopy after concentration of cells by cytospin and Giemsa staining. It was observed that the frequency of MoDCs expressing CD83, CD80, and CD86 as well as the MFI of HLA-DR were smaller in the group of patients with schistosomiasis compared to the group of patients with leishmaniasis. On the other hand, the frequency of IL-10R on MoDCs was higher in patients with schistosomiasis than in patients with leishmaniasis. CD4+ and CD8+ T lymphocytes from patients with schis-tosomiasis presented a lower frequency of CD28 and a higher frequency of CTLA-4 compared to lymphocytes from patients with leishmaniasis. Levels of IL-10 were higher in the supernatants of co-cultures from individuals with schistosomiasis compared to those with leishmaniasis. However, levels of TNF, IL-12p40, and IFN-γ were lower in the group of individuals with schistosomiasis. Regarding the frequency of MoDCs infected by L. braziliensis after 72 h in culture, it was observed that higher frequencies of cells from patients with schistosomiasis were infected compared to cells from patients with leishmaniasis. It was concluded that MoDCs from patients with schistosomiasis are more likely to be infected by L. braziliensis, possibly due to a lower degree of activation and a regulatory profile.

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“…Recently, we demonstrated that the recombinant S. mansoni antigens Sm29 SmTSP-2, and also PIII down-modulated the production of IFN- γ and TNF in a group of cutaneous leishmaniasis (CL) patients [8]. In the present study, these antigens were tested regarding their ability to alter the monocyte and lymphocyte profiles.…”

Section: Introductionmentioning

confidence: 91%

Changes in T-Cell and Monocyte PhenotypesIn VitrobySchistosoma mansoniAntigens in Cutaneous Leishmaniasis Patients

Bafica

Cardoso

Oliveira

et al. 2012

Journal of Parasitology Research

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High levels of proinflammatory cytokines such as IFN-γ and TNF are associated with tissue lesions in cutaneous leishmaniasis (CL). We previously demonstrated that Schistosoma mansoni antigens downmodulate the in vitro cytokine response in CL. In the current study we evaluated whether S. mansoni antigens alter monocyte and T-lymphocyte phenotypes in leishmaniasis. Peripheral blood mononuclear cells of CL patients were cultured with L. braziliensis antigen in the presence or absence of the S. mansoni antigens rSm29, rSmTSP-2- and PIII. Cells were stained with fluorochrome conjugated antibodies and analyzed by flow cytometry. The addition of rSm29 to the cultures decreased the expression of HLA-DR in nonclassical (CD14+CD16++) monocytes, while the addition of PIII diminished the expression of this molecule in classical (CD14++CD16−) and intermediate (CD14++CD16+) monocytes. The addition of PIII and rSmTSP-2 resulted in downmodulation of CD80 expression in nonclassical and CD86 expression in intermediate monocytes, respectively. These two antigens increased the expression of CTLA-4 in CD4+ T cells and they also expanded the frequency of CD4+CD25highFoxp3+ T cells. Taken together, we show that S. mansoni antigens, mainly rSmTSP-2 and PIII, are able to decrease the activation status of monocytes and also to upregulate the expression of modulatory molecules in T lymphocytes.

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Schistosoma mansoni antigens alter the cytokine response in vitro during cutaneous leishmaniasis

Cited by 13 publications

References 36 publications

<b><i>Schistosoma</i></b> Antigens Downmodulate the in vitro Inflammatory Response in Individuals Infected with Human T Cell Lymphotropic Virus Type 1

<b><i>Schistosoma</i></b> Antigens Downmodulate the in vitro Inflammatory Response in Individuals Infected with Human T Cell Lymphotropic Virus Type 1

Susceptibility of dendritic cells from individuals with schistosomiasis to infection by Leishmania braziliensis

Changes in T-Cell and Monocyte PhenotypesIn VitrobySchistosoma mansoniAntigens in Cutaneous Leishmaniasis Patients

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