Schistosoma japonicum: proteomics analysis of differentially expressed proteins from ultraviolet-attenuated cercariae compared to normal cercariae

Abstract

Schistosomiasis is considered the most important human helminthiasis in terms of morbidity and mortality. In this study, comparative soluble proteomic analysis of normal cercariae and ultraviolet-irradiated attenuated cercariae (UVAC) from Schistosoma japonicum were carried out in view of the high efficiency of irradiation-attenuated cercariae vaccine. The results revealed that some proteins showed significant differential expression in the parasite after treatment with ultraviolet light. Total 20 protein spots were identified by mass spectrometry, corresponded to five groups according to their functions in the main that were structural and motor proteins (actin, et al.), energy metabolism associated enzymes (glyceraldehydes-3-phosphage dehydrogenase, et al.), signaling transduction pathway-associated molecules (14-3-3 protein, et al.), heat shock protein families (HSP 70 family, et al.), and other functional proteins (20S proteasome). Furthermore, our results indicated that the differential expression of the proteins by ultraviolet irradiation may be, at least partially, acquired by regulating the mRNA levels of corresponding proteins. These results may provide new clues for further exploring the mechanism of protective immunity induced by UVAC and may shed some light on the development of vaccines against schistosomiasis.

Schistosomiasis in the People's Republic of China: the Era of the Three Gorges Dam

“…The landmark availability of the complete sequences of the S. japonicum (171) and S. mansoni (18) genomes will provide the necessary ancillary information. As well, new approaches in antigen discovery through the generation of a large schistosome transcriptome database, gene finding, and the explosion in postgenome technologies, including DNA microarray profiling, proteomics, glycomics, immunomics, and the application of RNA interference (RNAi) and novel imaging techniques (2,24,25,41,53,72,73,75,76,85,95,109,126,127,179,189,197,205,219,224,230), provide an unprecedented opportunity to identify a new generation of vaccine target molecules that may induce greater potency than the current candidate schistosome antigens (138).…”

Kinetics of circulating antigen 14-3-3 in sera of rabbits firstly infected with Schistosoma japonicum and treated with/without praziquantel

“…It has been shown that detection of schistosome circulating antigen (CA) is an effective approach to discriminate between previous exposure and current infection (Zhu 2005). There were many reports showing that 14-3-3 of S. japonicum was abundant in excretory-secretory antigen ), soluble egg antigen (Yang et al 2009) and adult worm antigen , and has an important value in diagnosis of acute and chronic infections of Schistosoma japonicum (Luo et al 2009); however, the kinetics of 14-3-3 in the sera of rabbits infected with S. japonicum and then treated with praziquantel remains unclear. The current study, therefore, was carried out to explore its dynamic changes.…”

Characterization of Schistosoma japonicum tetraspanning orphan receptor and its role in binding to complement C2 and immunoprotection against murine schistosomiasis

“…As snail control is always difficult to achieve [6], and treatment with praziquantel does not prevent a high frequency of reinfection [7], schistosomiasis is difficult to control. Thus, to control this disease, there is an urgent need to develop alternative therapies and effective vaccines [8] that could reduce the release of eggs and restrict parasite transmission or induce which kind of activity against schistosome infection.…”