Schinopsis brasiliensis Engl. to combat the biofilm-dependents diseases in vitro

Abstract

Dental caries and periodontal disease are the most prevalent of the biofilmdependent diseases. With numerous side effects on the use of chlorhexidine, the search for new safe therapeutic alternatives for microorganisms involved with these diseases increases every day. This study aimed to evaluate the antimicrobial activity and cytotoxicity of extracts made from the bark of Schinopsis brasiliensis Engl. against five oral microorganisms and analyze their phytochemical and thermal degradation profile. The liquid-liquid partition was performed with hexane, chloroform and ethyl acetate. The identification and quantification of the chemical marker was done. Antimicrobial activity was evaluated based on the minimum inhibitory concentration. The cytotoxicity was analyzed based on the hemolysing potential of the samples. The thermal degradation profile was performed by two different methods. Gallic acid was identified as the main compound of the samples and showed the highest amount in the chloroform fraction. All samples were able to inhibit the growth of the microorganisms tested and showed no cytotoxicity. The ethanol extract absorbs less heat than the fractions. All samples exhibited exothermic peak consistent with degradation of gallic acid. Based on the results, the samples used are potential candidates for use in dental formulations for biofilm control.

Gallic acid induces osteoblast differentiation and alleviates inflammatory response through GPR35/GSK3β/β‐catenin signaling pathway in human periodontal ligament cells

“…GA has been confirmed to have various biological activities, such as Alzheimer's disease, Parkinson's disease, sedative, cerebral ischemic and anti‐inflammatory, and cancer, gastrointestinal and cardiovascular diseases 9–11 . In addition, recently, in biofilm‐dependent diseases such as dental caries and periodontal disease, GA has been shown to have antibacterial activity against oral microorganisms 12 . However, studies on the direct effect of GA on periodontitis have not yet been reported.…”

“…[9][10][11] In addition, recently, in biofilm-dependent diseases such as dental caries and periodontal disease, GA has been shown to have antibacterial activity against oral microorganisms. 12 However, studies on the direct effect of GA on periodontitis have not yet been reported. G-protein-coupled receptors (GPCRs) remain the best studied class of cell surface receptors for novel small molecule drug discovery.…”