scCO2-foamed silk fibroin aerogel/poly(ε-caprolactone) scaffolds containing dexamethasone for bone regeneration

Goimil, Leticia; Santos‐Rosales, Víctor; Delgado, Araceli; Évora, Carmen; Reyes, Ricardo; Lozano-Pérez, Antonio Abel; Aznar-Cervantes, Salvador D.; Cenis, J. L.; Gómez-Amoza, J.L.; Concheiro, Angel; García‐González, Carlos A.

doi:10.1016/j.jcou.2019.02.016

Cited by 51 publications

(24 citation statements)

References 69 publications

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“…Other supercritical foaming modifications not requiring downstream processes are under research. Namely, the use of organic solvents [118,119], biofunctional plasticizers [114], or the incorporation of aerogels from different sources [120][121][122][123] in synthetic scaffolds processed by supercritical foaming promoted the formation of more interconnected porous structures with larger pores. The pore interconnectivity can be also modulated by modifications on the venting rate [124].…”

Section: Compressed Co 2 and Supercritical Co 2 -Assisted Foamingmentioning

confidence: 99%

“…Although it is a complex dynamic process where many parameters affect the ending structure, supercritical CO 2 foaming has been established as a versatile and efficient technology for the production of solvent-free scaffolds with remarkable in vivo outcomes [106,127,128]. Namely, supercritical foaming allowed the simultaneous processing of PCL scaffolds coupled with the loading of dexamethasone (up to 5 wt %), showing excellent in vivo compatibility and promoting the bone repair at 14 weeks post-implantation on a critical-sized calvarial defect using a rat model [121] (Figure 10). Furthermore, the bone in-growth in a β-tricalcium phosphate/PLA composite scaffold after 12 months post-implantation in sheep femur and tibia was demonstrated [129].…”

Section: Compressed Co 2 and Supercritical Co 2 -Assisted Foamingmentioning

confidence: 99%

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Solvent-Free Approaches for the Processing of Scaffolds in Regenerative Medicine

2020

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The regenerative medicine field is seeking novel strategies for the production of synthetic scaffolds that are able to promote the in vivo regeneration of a fully functional tissue. The choices of the scaffold formulation and the manufacturing method are crucial to determine the rate of success of the graft for the intended tissue regeneration process. On one hand, the incorporation of bioactive compounds such as growth factors and drugs in the scaffolds can efficiently guide and promote the spreading, differentiation, growth, and proliferation of cells as well as alleviate post-surgical complications such as foreign body responses and infections. On the other hand, the manufacturing method will determine the feasible morphological properties of the scaffolds and, in certain cases, it can compromise their biocompatibility. In the case of medicated scaffolds, the manufacturing method has also a key effect in the incorporation yield and retained activity of the loaded bioactive agents. In this work, solvent-free methods for scaffolds production, i.e., technological approaches leading to the processing of the porous material with no use of solvents, are presented as advantageous solutions for the processing of medicated scaffolds in terms of efficiency and versatility. The principles of these solvent-free technologies (melt molding, 3D printing by fused deposition modeling, sintering of solid microspheres, gas foaming, and compressed CO2 and supercritical CO2-assisted foaming), a critical discussion of advantages and limitations, as well as selected examples for regenerative medicine purposes are herein presented.

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Section: Compressed Co 2 and Supercritical Co 2 -Assisted Foamingmentioning

confidence: 99%

Section: Compressed Co 2 and Supercritical Co 2 -Assisted Foamingmentioning

confidence: 99%

Solvent-Free Approaches for the Processing of Scaffolds in Regenerative Medicine

2020

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“…Accordingly, the passive release profile from unstimulated electrodes were evaluated over the same period of 300 min. Figure 5a shows the passive release profile of Dx as a function of square root of time according to the Higuchi model for the drug release from a polymer film [27,50], where pure Fickian diffusion is the dominant phenomena [48]. The low diffusion value, in the beginning of the process, may depend on the slow penetration of supportive electrolyte into the polymeric film [49].…”

Section: Molecules 2020 25 X For Peer Review 6 Of 13mentioning

confidence: 99%

“…Dexamethasone (Dx) is a synthetic glucocorticoid that reduces inflammation in the central nervous system, acting through glucocorticoid receptors found in most neurons and glial cells. Due to being locally delivered, the specificity and efficiency of dexamethasone means that only small amounts of the drug are required [13,22,[24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Polysaccharide κ-Carrageenan as Doping Agent in Conductive Coatings for Electrochemical Controlled Release of Dexamethasone at Therapeutic Doses

et al. 2020

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Smart conductive materials are developed in regenerative medicine to promote a controlled release profile of charged bioactive agents in the vicinity of implants. The incorporation and the active electrochemical release of the charged compounds into the organic conductive coating is achieved due to its intrinsic electrical properties. The anti-inflammatory drug dexamethasone was added during the polymerization, and its subsequent release at therapeutic doses was reached by electrical stimulation. In this work, a Poly (3,4-ethylenedioxythiophene): κ-carrageenan: dexamethasone film was prepared, and κ-carrageenan was incorporated to keep the electrochemical and physical stability of the electroactive matrix. The presence of κ-carrageenan and dexamethasone in the conductive film was confirmed by µ-Raman spectroscopy and their effect in the topographic was studied using profilometry. The dexamethasone release process was evaluated by cyclic voltammetry and High-Resolution mass spectrometry. In conclusion, κ-carrageenan as a doping agent improves the electrical properties of the conductive layer allowing the release of dexamethasone at therapeutic levels by electrochemical stimulation, providing a stable system to be used in organic bioelectronics systems.

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“…The outstanding textural properties of the aerogels have also attracted the attention from other fields such as biomedical and environmental sciences [3][4][5][6]. Biomedical applications of aerogels include the encapsulation of bioactive agents with solubility or stability limitations, and their use as synthetic scaffolds for tissue engineering and wound dressing materials for chronic wounds [7][8][9][10][11][12][13][14][15][16]. In the latter case, the large surface area of the aerogels confers them the 2 of 13 ability to load and release bioactive agents, such as antibiotics or growth factors, which can facilitate the wound healing process [17].…”

Section: Introductionmentioning

confidence: 99%

Jet Cutting Technique for the Production of Chitosan Aerogel Microparticles Loaded with Vancomycin

et al. 2020

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Biopolymer-based aerogels can be obtained by supercritical drying of wet gels and endowed with outstanding properties for biomedical applications. Namely, polysaccharide-based aerogels in the form of microparticles are of special interest for wound treatment and can also be loaded with bioactive agents to improve the healing process. However, the production of the precursor gel may be limited by the viscosity of the polysaccharide initial solution. The jet cutting technique is regarded as a suitable processing technique to overcome this problem. In this work, the technological combination of jet cutting and supercritical drying of gels was assessed to produce chitosan aerogel microparticles loaded with vancomycin HCl (antimicrobial agent) for wound healing purposes. The resulting aerogel formulation was evaluated in terms of morphology, textural properties, drug loading, and release profile. Aerogels were also tested for wound application in terms of exudate sorption capacity, antimicrobial activity, hemocompatibility, and cytocompatibility. Overall, the microparticles had excellent textural properties, absorbed high amounts of exudate, and controlled the release of vancomycin HCl, providing sustained antimicrobial activity.

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scCO2-foamed silk fibroin aerogel/poly(ε-caprolactone) scaffolds containing dexamethasone for bone regeneration

Cited by 51 publications

References 69 publications

Solvent-Free Approaches for the Processing of Scaffolds in Regenerative Medicine

Solvent-Free Approaches for the Processing of Scaffolds in Regenerative Medicine

Polysaccharide κ-Carrageenan as Doping Agent in Conductive Coatings for Electrochemical Controlled Release of Dexamethasone at Therapeutic Doses

Jet Cutting Technique for the Production of Chitosan Aerogel Microparticles Loaded with Vancomycin

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