SCCmec IX in meticillin-resistant Staphylococcus aureus and meticillin-resistant coagulase-negative staphylococci from pigs and workers at pig farms in Khon Kaen, Thailand

Sinlapasorn, Siwaporn; Lulitanond, Aroonlug; Chanawong, Aroonwadee; Wilailuckana, Chotechana; Tavichakorntrakool, Ratree; Chindawong, Kanoksak; Seelaget, Charinya; Krasaesom, Mana; Chartchai, Sarawut; Wonglakorn, Lumyai; Sribenjalux, Pipat

doi:10.1099/jmm.0.000119

Cited by 24 publications

(29 citation statements)

References 30 publications

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“…A relatively high frequency of ccrC positive SCCmec was found in S. hominis, which suggests that S. hominis could serve as a reservoir for this type of SCCmec for S. aureus (Mendoza-Olazaran et al, 2013). A pseudo (Ψ) SCC element with a truncated ccrA gene located in the left extremity of the SCCmec identified in this study showed high similarity to a ΨSCC element found in type IX SCCmec of a ST9 MRSA isolate from Thailand (Anukool et al, 2011;Lulitanond et al, 2013;Sinlapasorn et al, 2015).…”

Section: Discussionmentioning

confidence: 56%

Genetic features of livestock-associated Staphylococcus aureus ST9 isolates from Chinese pigs that carry the lsa(E) gene for quinupristin/dalfopristin resistance

Yan

Chlebowicz

et al. 2016

International Journal of Medical Microbiology

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Whole-genome sequencing (WGS) was used to investigate the genetic features of the recently identified lsa(E) gene in porcine S. aureus ST9 isolates. Three quinupristin/dalfopristin-resistant isolates harboring the lsa(E) gene (two MRSA and one MSSA) were sequenced. Phylogenetic analysis of 184S. aureus genomes showed that ST9 porcine isolates belong to a distinct sequence cluster. Further analysis showed that all isolates were deficient in the recently described type IV restriction-modification system and SCCmec type XII was identified in the two MRSA isolates, which included a rare class C2 mec gene complex. A 24kb ΨSCC fragment was found in the MRSA and MSSA isolates sharing 99% nucleotide sequence homology with the ΨSCCJCSC6690 (O-2) element of a ST9 MRSA isolate from Thailand (accession number AB705453). Comparison of these ST9 isolates with 181 publically available S. aureus genomes identified 24 genes present in all (100%) ST9 isolates, that were absent from the most closely related human isolate. Our analysis suggests that the sequenced quinupristin/dalfopristin-resistant ST9 lineage represent a reservoir of mobile genetic elements associated with resistance and virulence features.

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Section: Discussionmentioning

confidence: 56%

Genetic features of livestock-associated Staphylococcus aureus ST9 isolates from Chinese pigs that carry the lsa(E) gene for quinupristin/dalfopristin resistance

Yan

Chlebowicz

et al. 2016

International Journal of Medical Microbiology

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“…In five publications from Thailand, methicillin resistant Staphylococcus aureus (MRSA) was investigated in pigs and pork meat [89,90,91,92,93]. In one study, a total of 104 pig farms were investigated for MRSA, alongside farmers and the farms′ environment.…”

Section: Resultsmentioning

confidence: 99%

“…The herd-level prevalence of MRSA was ~9.6%, and the individual pig and farmer prevalence was 0.7% and 2.5%, respectively [93]. All (100%) MRSA isolates were resistant to clindamycin, oxytetracycline, and tetracycline, while 100% were susceptible to vancomycin [92,93]. The Staphylococcal Cassette Chromosome mec (SCC mec ) clonal type IX appears to be quite disseminated in the Thai pig industry, representing a reservoir for human infection [91,92].…”

Section: Resultsmentioning

confidence: 99%

“…All (100%) MRSA isolates were resistant to clindamycin, oxytetracycline, and tetracycline, while 100% were susceptible to vancomycin [92,93]. The Staphylococcal Cassette Chromosome mec (SCC mec ) clonal type IX appears to be quite disseminated in the Thai pig industry, representing a reservoir for human infection [91,92]. A study in Vietnam investigated AMR in 45 Streptococcus suis serotype 2 isolates from pig tonsils.…”

Section: Resultsmentioning

confidence: 99%

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Antimicrobial Usage and Antimicrobial Resistance in Animal Production in Southeast Asia: A Review

et al. 2016

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Southeast Asia is an area of great economic dynamism. In recent years, it has experienced a rapid rise in the levels of animal product production and consumption. The region is considered to be a hotspot for infectious diseases and antimicrobial resistance (AMR). We reviewed English-language peer-reviewed publications related to antimicrobial usage (AMU) and AMR in animal production, as well as antimicrobial residues in meat and fish from 2000 to 2016, in the region. There is a paucity of data from most countries and for most bacterial pathogens. Most of the published work relates to non-typhoidal Salmonella (NTS), Escherichia coli (E. coli), and Campylobacter spp. (mainly from Vietnam and Thailand), Enterococcus spp. (Malaysia), and methicillin-resistant Staphylococcus aureus (MRSA) (Thailand). However, most studies used the disk diffusion method for antimicrobial susceptibility testing; breakpoints were interpreted using Clinical Standard Laboratory Institute (CSLI) guidelines. Statistical models integrating data from publications on AMR in NTS and E. coli studies show a higher overall prevalence of AMR in pig isolates, and an increase in levels of AMR over the years. AMU studies (mostly from Vietnam) indicate very high usage levels of most types of antimicrobials, including beta-lactams, aminoglycosides, macrolides, and quinolones. This review summarizes information about genetic determinants of resistance, most of which are transferrable (mostly plasmids and integrons). The data in this review provide a benchmark to help focus research and policies on AMU and AMR in the region.

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“…We also did not find type VII SCC mec in CoNS in this study as other researchers; however, one isolate of type IX was disclosed in S. haemolyticus in clinic, which had not been previously confirmed in CoNS [ 12 , 23 ]. A recent study also reported SCC mec type IX in CoNS in the community [ 24 ]. Significantly, most CoNS strains recovered in this study (clinic and community) consisted of untypable SCC mec elements as many other reports [ 14 – 16 , 23 , 25 ].…”

Section: Discussionmentioning

confidence: 98%

Extreme diversity and multiple SCCmec elements in coagulase-negative Staphylococcus found in the Clinic and Community in Beijing, China

Chen

Zheng

et al. 2017

Ann Clin Microbiol Antimicrob

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BackgroundCoagulase-negative staphylococci (CoNS) are recognized as a large reservoir of staphylococcal cassette chromosome mec (SCCmec) harboured by Staphylococcus aureus. However, data of SCCmec in CoNS are relatively absent particularly in China.MethodsSeventy-eight CoNS clinical and 47 community isolates were collected in Beijing. PCR was performed to classify SCCmec types. Under oxacillin treatment, quantitative real-time reverse transcription PCR (qRT-PCR) was performed to compare mecA mRNA levels and mRNA half-life between isolates with single SCCmec element and those with multiple one. Their growth curves were analysed. Their bacterial cell wall integrity was also compared by performing a Gram stain. All ccr complex segments were sequenced and obtained ccr segments were analysed by phylogenetic analyses.ResultsAll 78 clinical isolates had mecA segments compared with 38% in community isolates (total 47). Only 29% clinical isolates and 33% community isolates (among mecA positive isolates) harboured a single previously identified SCCmec type; notably, 17% clinical isolates and 28% community isolates had multiple SCCmec types. Further studies indicated that isolates with multiple SCCmec elements had more stable mecA mRNA expression compared with isolates with single SCCmec elements. CoNS with multiple SCCmec elements demonstrated superior cell wall integrity. Interestingly, phylogenetic analyses of obtained 70 ccr segments indicated that horizontal gene transfer of the ccr complex might exist among various species of clinical CoNS, community CoNS and S. aureus.ConclusionsCoNS recovered from patients carried extremely diverse but distinctive SCCmec elements compared with isolates from the community. More attention should be given to CoNS with multiple SCCmec not only because they had superior cell wall integrity, but also because CoNS and S. aureus might acquire multiple SCCmec through the ccr complex.Electronic supplementary materialThe online version of this article (doi:10.1186/s12941-017-0231-z) contains supplementary material, which is available to authorized users.

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SCCmec IX in meticillin-resistant Staphylococcus aureus and meticillin-resistant coagulase-negative staphylococci from pigs and workers at pig farms in Khon Kaen, Thailand

Cited by 24 publications

References 30 publications

Genetic features of livestock-associated Staphylococcus aureus ST9 isolates from Chinese pigs that carry the lsa(E) gene for quinupristin/dalfopristin resistance

Genetic features of livestock-associated Staphylococcus aureus ST9 isolates from Chinese pigs that carry the lsa(E) gene for quinupristin/dalfopristin resistance

Antimicrobial Usage and Antimicrobial Resistance in Animal Production in Southeast Asia: A Review

Extreme diversity and multiple SCCmec elements in coagulase-negative Staphylococcus found in the Clinic and Community in Beijing, China

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