Scavenger Receptor Class B, Type I, a CD36 Related Protein inMacrobrachium nipponense: Characterization, RNA Interference, and Expression Analysis with Different Dietary Lipid Sources

Abstract

The scavenger receptor class B, type I (SR-BI), is a member of the CD36 superfamily comprising transmembrane proteins involved in mammalian and fish lipid homeostasis regulation. We hypothesize that this receptor plays an important role in Macrobrachium nipponense lipid metabolism. However, little attention has been paid to SR-BI in commercial crustaceans. In the present study, we report a cDNA encoding M. nipponense scavenger receptor class B, type I (designated as MnSR-BI), obtained from a hepatopancreas cDNA library. The complete MnSR-BI coding sequence was 1545 bp, encoding 514 amino acid peptides. The MnSR-BI primary structure consisted of a CD36 domain that contained two transmembrane regions at the N- and C-terminals of the protein. SR-BI mRNA expression was specifically detected in muscle, gill, ovum, intestine, hepatopancreas, stomach, and ovary tissues. Furthermore, its expression in the hepatopancreas was regulated by dietary lipid sources, with prawns fed soybean and linseed oils exhibiting higher expression levels. RNAi-based SR-BI silencing resulted in the suppression of its expression in the hepatopancreas and variation in the expression of lipid metabolism-related genes. This is the first report of SR-BI in freshwater prawns and provides the basis for further studies on SR-BI in crustaceans.

A scavenger receptor B (CD36)-like protein is a potential mediator of intestinal heme absorption in the hematophagous ectoparasite Lepeophtheirus salmonis

“…This argument could contribute to explain increased down-regulation under prolonged starvation. In accordance with our findings, a nutrient induced positive feedback mechanism was observed in the crustacean Macrobrachium nipponense , where a scavenger receptor was up-regulated by various dietary lipid sources 38 . Further, Staron et al .…”

A Novel Candidate Gene Associated With Body Weight in the Pacific White Shrimp Litopenaeus vannamei

“…The similar function of SRs was also revealed in invertebrates. For example, in Macrobrachium nipponense , the expression of gene encoding the class B SR can be regulated by dietary lipid sources including soybean and linseed oils (Ding et al, 2016). Therefore, it’s interesting to note that SRC may be related to the body weight of shrimp by participating in lipid metabolism.…”

Thematic Review Series: Lipid Transfer Proteins Scavenger receptor B type 1: expression, molecular regulation, and cholesterol transport function

“…High expression of SR-B1 is reported in the liver, ovary, heart, and blood vessels in the turtle and in the liver of chickens, frogs, goldfish, sharks, and skates (93). SR-B1 expression is reported in the liver of Atlantic salmon (Salmo salar L.) (94) and in several organs of prawns (Macrobrachium nipponense) (95). Twelve to fourteen candidate SNMP/ CD36 homologs from each of the genomes of Drosophila melanogaster, Drosophila pseudoobscura, Anopheles gambiae, and Aedes aegypti (Diptera), eight candidate homologs from Apis mellifera (Hymenoptera), and 15 from Tribolium castaneum (Coleoptera) have been identified (96,97).…”