Scatter‐limited phototherapy: A model for laser treatment of skin

Reinisch, Lou

doi:10.1002/lsm.10046

Cited by 26 publications

(25 citation statements)

References 14 publications

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“…In addition, the wavelength range in between 600 and 650nm (red light), as utilized herein, is able to penetrate through the epidermis and dermis, reaching approximately from 1.0 and up to 2.0mm depth, which certainly fits the purpose of superficial skin healing that we desired with this methodology [5]; we can also assume that a certain spread of the laser light happened by scattering [45, 46], better distributing the light through the cells either in vivo or in vitro .…”

Section: Discussionmentioning

confidence: 99%

Low‐level laser irradiation promotes the proliferation and maturation of keratinocytes during epithelial wound repair

Sperandio

Simões

Corrêa

et al. 2014

Journal of Biophotonics

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Low-level laser therapy (LLLT) has been extensively employed to improve epithelial wound healing, though the exact response of epithelium maturation and stratification after LLLT is unknown. Thus, this study aimed to assess the in vitro growth and differentiation of keratinocytes (KCs) and in vivo wound healing response when treated with LLLT. Human KCs (HaCaT cells) showed an enhanced proliferation with all the employed laser energy densities (3, 6 and 12 J/cm2, 660nm, 100mW), together with an increased expression of Cyclin D1. Moreover, the immunoexpression of proteins related to epithelial proliferation and maturation (p63, CK10, CK14) all indicated a faster maturation of the migrating KCs in the LLLT-treated wounds. In that way, an improved epithelial healing was promoted by LLLT with the employed parameters; this improvement was confirmed by changes in the expression of several proteins related to epithelial proliferation and maturation.

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Section: Discussionmentioning

confidence: 99%

Low‐level laser irradiation promotes the proliferation and maturation of keratinocytes during epithelial wound repair

Sperandio

Simões

Corrêa

et al. 2014

Journal of Biophotonics

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“…Various types of non-ablative lasers have been used [23]. While skin resurfacing with intense pulsed light or radiofrequency waves causes no epidermal damage at all, the term nonablative resurfacing has a different meaning with Er:YAG resurfacing-the epidermis is in reality damaged, but not removed, and acts as a wound dressing [13][14][15][16].…”

Section: Discussionmentioning

confidence: 99%

“…In both, the problem of epidermal ablation with its implication of longer healing and possible side effects is present [3][4][5][6][7][8][9][10][11][12]. With the introduction of non-ablative and non-coherent light skin resurfacing methods a stimulus has been released to further improve what are nowadays largely used laser resurfacing techniques [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Repetitive Er:YAG laser irradiation of human skin: A histological evaluation

Drnovšek-Olup

Beltram

Pižem³

2004

Lasers Surg Med

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“…This calculation was insufficient to remove out-of-plane fluorescence as it did not take into account scattering. Reinisch et al 21 discussed that in a highly scattering media, such as tissue, and at a similar scale, scatter can be modeled by a Gaussian. We modify Eq.…”

Section: Introductionmentioning

confidence: 99%

Removal of Out-of-Plane Fluorescence for Single Cell Visualization and Quantification in Cryo-Imaging

et al. 2009

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We developed a cryo-imaging system, which alternates between sectioning (10–40 μm) and imaging bright field and fluorescence block-face image volumes with micronscale-resolution. For applications requiring single-cell detection of fluorescently labeled cells anywhere in a mouse, we are developing software for reduction of out-of-plane fluorescence. In mouse experiments, we imaged GFP-labeled cancer and stem cells, and cell-sized fluorescent microspheres. To remove out-of-plane fluorescence, we used a simplified model of light-tissue interaction whereby the next-image was scaled, blurred, and subtracted from the current image. We estimated scaling and blurring parameters by minimizing an objective function on subtracted images. Tissue-specific attenuation parameters [μT: heart (267 ± 47.6 cm−1), liver (218 ± 27.1 cm−1), brain (161 ± 27.4 cm−1)] were found to be within the range of estimates in the literature. “Next-image” processing removed out-of-plane fluorescence equally well across multiple tissues (brain, kidney, liver, etc.), and analysis of 200 microsphere images gave 97 ± 2% reduction of out-of-plane fluorescence. Next-image processing greatly improved axial-resolution, enabled high quality 3D volume renderings, and improved automated enumeration of single cells by up to 24%. The method has been used to identify metastatic cancer sites, determine homing of stem cells to injury sites, and show microsphere distribution correlated with blood flow patterns.

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Scatter‐limited phototherapy: A model for laser treatment of skin

Cited by 26 publications

References 14 publications

Low‐level laser irradiation promotes the proliferation and maturation of keratinocytes during epithelial wound repair

Low‐level laser irradiation promotes the proliferation and maturation of keratinocytes during epithelial wound repair

Repetitive Er:YAG laser irradiation of human skin: A histological evaluation

Removal of Out-of-Plane Fluorescence for Single Cell Visualization and Quantification in Cryo-Imaging

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