SCAR, RAPD and RFLP markers linked to a dominant gene (Are) conferring resistance to anthracnose in common bean

Abstract

Anthracnose, caused by the fungusColletotrichum lindemuthianum, is a severe disease of common bean (Phaseolus vulgaris L.) controlled, in Europe, by a single dominant gene,Are. Four pairs of near-isogenic lines (NILs) were constructed, in which theAre gene was introgressed into different genetic backgrounds. These pairs of NILs were used to search for DNA markers linked to the resistance gene. Nine molecular markers, five RAPDs and four RFLPs, were found to discriminate between the resistant and the susceptible members of these NILs. A backcross progeny of 120 individuals was analysed to map these markers in relation to theAre locus. Five out of the nine markers were shown to be linked to theAre gene within a distance of 12.0 cM. The most tightly linked, a RAPD marker, was used to generate a pair of primers that specifically amplify this RAPD (sequence characterized amplified region, SCAR).

Molecular Analysis of a Large Subtelomeric Nucleotide-Binding-Site–Leucine-Rich-Repeat Family in Two Representative Genotypes of the Major Gene Pools of Phaseolus vulgaris

“…Indeed, on the basis of comparative mapping studies, the region containing P-PRLJ1.a and -f (block 2 on Figure 1B) is known to harbor the Co-RVI anthracnose resistance gene (Adam-Blondon et al 1994;Freyre et al 1998). Altogether, the end of the short arm of chromosome 4 carries an important collection of specific R genes and R QTL effective against diverse pathogens [fungi C. lindemuthianum and Uromyces appendiculatus, bacteria Pseudomonas syringae pv phaseolicola, and bean golden yellow mosaic virus (BGYMV)] (Lopez et al 2003;Miklas et al 2006;Rodriguez-Suarez et al 2008).…”

Towards an integrated linkage map of common bean. 4. Development of a core linkage map and alignment of RFLP maps

“…Alignments with other maps could be established using PCR-based markers. SCAR markers have been located on linkage groups 2 (near I, Melotto et al 1996), 6 (near bc-3, Johnson et al 1997), 8 and10 (Jlks andJlds, respectively;Adam-Blondon 1994), and 11 (H20s and F3s, Adam-Blondon et al 1994a). In addition, clones with known sequences have been mapped on linkage groups 1 (Pal-1), 2 (PvPR-2, PGIP, Cel, ChS and ChS-2, »pe-2, and ºri-3), 3 (PGRP1.8-3, PvPR-1, and SS), 4 (¸ec-Arl-AI, Hrgp4-1, and Cab-1), 5 (¸ox-1), 6 (Hsp70, Cdc-2), 7 (ChI, Ef,¸ec-2 and¸ec-3,¸egH, Per, Phs, ºri-2), 8 (GS-c and¸ox-2), 9 (Gluc, Ch, Cad), and 10 (»pe-3) as shown in Fig.…”

“…These SCARs were developed by the Paris group and were derived from bands produced by Operon primers F3, J1, and H20 (AdamBlondon 1994; Adam-Blondon et al 1994a). Cycling conditions were 1 cycle of 2 min at 94°C; 40 cycles of 1 min at 94°C, 1 min at 60°C, and 2 min at 72°C; followed by 5 min at 72°C.…”

Introgression and pyramiding into common bean market class fabada of genes conferring resistance to anthracnose and potyvirus

“…The following molecular markers were used: the dominant SCAR marker SW13 690 , linked to the I gene (Melotto et al 1996); the dominant SCAR marker ROC11 420 , linked in repulsion phase to the recessive bc-3 resistance allele (Johnson et al 1997); the dominant CAP marker SCH20 1000 (obtained after Taq I digestion of amplification product), the dominant RAPD fragments OM2 1050 , OQ04 600, and the SCAR SQ4 1440 , linked to cluster Co-2 (Adam- Blondon et al 1994;Rodríguez-Suárez et al 2007Awale et al 2008); the dominant SCAR markers SI19 460 , SB12 350 , linked to Co-3/9 cluster (Miklas et al 2000;Méndez de Vigo et al 2005;Rodríguez-Suárez et al 2008). Specific molecular markers were used in breeding programs once they were reported.…”