Introgression and pyramiding into common bean market class fabada of genes conferring resistance to anthracnose and potyvirus

Fernández, Juan José Ferreira; Čampa, Ana; Pérez-Vega, Elena; Rodríguez-Suárez, Cristina; Giráldez, R.

doi:10.1007/s00122-011-1746-x

Cited by 34 publications

(23 citation statements)

References 27 publications

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“…NIL derived from the anthracnose resistance sources A493 and A321 carried an introgressed common region at the beginning of chromosome Pv04. This position agrees with results that indicated that these NIL have resistance genes in the Co‐3 cluster (Ferreira et al, 2012; Méndez‐Vigo et al, 2005) and corroborated the physical positions of the linked markers Pv‐ctt001 and SW12. The data from a Genome‐Wide Association Study (GWAS) involving 230 Andean beans indicated that the resistance to races 7 and 109 also resides in the physical position 0.21 to 0.53 Mb of chromosome Pv04 (Zuiderveen et al, 2016).…”

Section: Discussionsupporting

confidence: 90%

Introgressed Genomic Regions in a Set of Near‐Isogenic Lines of Common Bean Revealed by Genotyping‐by‐Sequencing

2017

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Genotyping-by-sequencing (GBS) was used to investigate and identify the introgressed genomic regions that corresponded to resistance alleles for anthracnose (Co-2 and Co-3), bean common mosaic virus (BCMV), and bean common mosaic necrosis virus (BCMNV, I and bc-3) in a set of bean near-isogenic lines (NIL). The GBS analysis provided 12,697 single nucleotide polymorphisms (SNPs) although the densities along the chromosomes were not uniform, and some chromosomal regions, such as centromeric or pericentromeric regions, were less tagged. The backcrossing method resulted in the introgression of genomic regions into specific chromosomes. The number of introgressed region-tagging SNPs varied between 1 and 13, representing between 0.33 and 6.88% of the bean genome. The changes detected among the recurrent parent and NIL in chromosomal regions are candidate regions that may contain the introgressed genes. By comparing the NIL derived from the same resistance source, it was possible to delimit in chromosomes Pv02, Pv04, Pv06, and Pv11 the genomic regions containing the resistance genes I, . Results allowed verification of the physical positions of the resistance genes and a clearer physical position of the anthracnose resistance genes Co-2 and Co-3. Two nonoverlapping regions were delimited in chromosome Pv11 from common regions in NIL with resistance loci mapped to the Co-2 cluster. Alleles of the loci included within these genomic regions show strong linkage disequilibrium. This knowledge can be used in selection programs involving these regions rich in resistance genes.

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Section: Discussionsupporting

confidence: 90%

Introgressed Genomic Regions in a Set of Near‐Isogenic Lines of Common Bean Revealed by Genotyping‐by‐Sequencing

2017

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“…In Spain, for example, with this aim of local and cultural adaptation, new improved materials have been developed from landraces in tomatoes (Casals et al, 2010; Garcia-Martínez et al, 2011), beans (Bosch et al, 1998; Almirall et al, 2010; Ferreira et al, 2012), onions (Simó et al, 2012), and eggplants (Prohens et al, 2009). It would be difficult to argue that these cultivars should not be considered landraces because they have been obtained using breeding techniques like directed crossings and/or self-fertilization followed by selection.…”

Section: Landraces the New Generationmentioning

confidence: 99%

Toward an Evolved Concept of Landrace

Casañas¹,

Simó²,

Casals³

et al. 2017

Front. Plant Sci.

178

124

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The term “landrace” has generally been defined as a cultivated, genetically heterogeneous variety that has evolved in a certain ecogeographical area and is therefore adapted to the edaphic and climatic conditions and to its traditional management and uses. Despite being considered by many to be inalterable, landraces have been and are in a constant state of evolution as a result of natural and artificial selection. Many landraces have disappeared from cultivation but are preserved in gene banks. Using modern selection and breeding technology tools to shape these preserved landraces together with the ones that are still cultivated is a further step in their evolution in order to preserve their agricultural significance. Adapting historical landraces to present agricultural conditions using cutting-edge breeding technology represents a challenging opportunity to use them in a modern sustainable agriculture, as an immediate return on the investment is highly unlikely. Consequently, we propose a more inclusive definition of landraces, namely that they consist of cultivated varieties that have evolved and may continue evolving, using conventional or modern breeding techniques, in traditional or new agricultural environments within a defined ecogeographical area and under the influence of the local human culture. This includes adaptation of landraces to new management systems and the unconscious or conscious selection made by farmers or breeders using available technology. In this respect, a mixed selection system might be established in which farmers and other social agents develop evolved landraces from the variability generated by public entities.

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“…The gene pyramiding approaches of this study complements that of Ragagnin et al (2009) who succeeded using random amplified and polymorphic DNA (RAPD) and sequence-characterized amplified regions (SCAR) markers to pyramid resistance genes Co-4, Co-6, and Co-10 against anthracnose, Phg-1 against angular leaf spot, and Ur-ON for rust into the susceptible 'carioca' market class cultivar Rudá. Ferreira et al (2012) used SCAR, CAPS, and RAPD markers to successfully pyramid Co-2, Co-3/9 anthracnose and I and bc-3 common mosaic virus resistance genes into the 'fabada' market class A25 genotype. Ddamulira et al (2015) reported the effectiveness of gene pyramiding in improving angular leaf spot resistance in susceptible common bean cultivar.…”

Section: Gene Pyramiding and Cultivar Developmentmentioning

confidence: 99%

“…In soybean for mosaic virus disease resistance (SMV), researchers successfully pyramided three genes Rsv1, Rsv3 and Rsv4 with the aid of microsatellite markers in order to develop new soybean lines containing multiple resistance genes for soybean mosaic virus (SMV) resistance. Marker-assisted selection (MAS) and gene pyramiding have been reported in common bean research (Kelly et al, 2003, Miklas et al, 2006Ragagnin et al, 2009); Ferreira et al, 2012;. Recently, Ddamulira et al, (2015) reported the efficiency and effectiveness of gene pyramiding in improving angular leaf spot resistance in susceptible common bean cultivar.…”

Section: Introductionmentioning

confidence: 99%

Marker-assisted Pyramiding Resistance Genes Against Angular Leaf Spot and Common Bacterial Blight Disease into Preferred Common Bean Cultivar

Rezene¹,

Tesfaye

Mukankusi

et al. 2019

mpb

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Angular leaf spot (ALS) caused by Pseudocercospora griseola and common bacterial blight (CBB) caused by Xanthomonas campestris pv phaseoli X. campestris pv. phaseoli var. fuscans are the most economically important diseases of common bean production in Ethiopia. This research aims at pyramiding the Phg-2 R gene for angular leaf spot resistance and two CBB major resistance quantitative trait loci (RQTLs) into the background of the most popular and susceptible common bean cultivar "REDWOLAITA" (RW) with the aid of marker-assisted breeding method. Marker-assisted Parallel Back Crossing (MAPBC) breeding scheme with three separate parallel backcrossing streams were adopted for tracking three independent resistance loci linked to g796 (Phg-2 for ALS resistance) and, SU91 and SAP6 genetic markers from two different donor parents to the REDWOLAITA recurrent parent. The two donor parental lines VAX6 (with known RQTLs tagged by the SAP6 and SU91 genetic markers on linkage groups 10 and 8, respectively) and MEX54 with the Phg-2 R gene tagged by the g796 genetic marker at the linkage group 8 were used in the gene pyramiding program. After the BC4 generation, progenies that combined SAP6 and g796 genetic markers were created and selected from the BC4 inter-crossing of progenies. Then, further inter-crossing was made between selected progenies that combined the SAP6 and g796 genetic markers with selected progenies with the SU91 genetic marker. Finally, from this study we developed Monogenic Near Isogenic Lines (MNILs) with R genes tagged by the SAP6, g796, and SU91 molecular markers and polygenic PNILs with different gene combination includes MNIL SAP6 , MNIL SU91 & MNIL g796 , polygenetic PNILs SAP6/g796 , PNILs SU91/g796 , PNILs SAP6/SU , PNILs SAP6/g796/SAP6 , with more than 97% genome recovered from the RW genetic background. Marker-assisted backcrossing facilitated selection of progenies that combined good agronomic traits with resistance loci were constructed from the RW common bean cultivar genetic background and tested under the screening house condition. The developed lines showed high level of disease resistance to the strains of CBB and ALS present under the screening conditions. They were selected to be multiplied and tested under multiple environment, before varietal release and wider production. Developed MNILs with good agronomic background will also be used as alternative donor parent for the future gene pyramiding program.

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Introgression and pyramiding into common bean market class fabada of genes conferring resistance to anthracnose and potyvirus

Cited by 34 publications

References 27 publications

Introgressed Genomic Regions in a Set of Near‐Isogenic Lines of Common Bean Revealed by Genotyping‐by‐Sequencing

Introgressed Genomic Regions in a Set of Near‐Isogenic Lines of Common Bean Revealed by Genotyping‐by‐Sequencing

Toward an Evolved Concept of Landrace

Marker-assisted Pyramiding Resistance Genes Against Angular Leaf Spot and Common Bacterial Blight Disease into Preferred Common Bean Cultivar

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