Scanometry as microplate reader for high throughput method based on DPPH dry reagent for antioxidant assay

Hidayat, Moch. Amrun; Fitri, Aulia; Kuswandi, Bambang

doi:10.1016/j.apsb.2017.02.001

Cited by 33 publications

(15 citation statements)

References 34 publications

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“…Using the standard calibration curve of GA obtained by scanometry technique, the antioxidant capacity of each extract was calculated and expressed as gallic acid equivalent in mg/L (mg/L GAE) as described in our previous work [29]. Afterward, the results were compared with that obtained from the DPPH-spectrophotometry method.…”

Section: Application Of Antioxidant Sensor On the Plant Extractsmentioning

confidence: 99%

“…The immobilization of DPPH on the bottom of each well was performed by solvent evaporation as described in our previous work [29]. Here, the construction of the current DPPH-probe was simpler than our previous DPPH-probe, as the pharmaceutical blister was used to substitute a micro-well plate as a solid support (Fig.…”

Section: Antioxidant Sensor Set-upmentioning

confidence: 99%

“…In our previous work, the DPPH solution was immobilized as a dry reagent on a microplate well as a sensing probe [29]. The antioxidant assay was simply performed by addition of ethanolic or methanolic plant extracts to the DPPH-probe.…”

Section: Introductionmentioning

confidence: 99%

“…In this regard, the current work aimed to develop simple antioxidant sensors based on DPPH with a scanometry assay for antioxidant determination in plant extracts. Here, the DPPH solution was immobilized as dry reagents [29] by a surface coating on the blister sheet as the sensing probes. Then, the sensor was set up as scanometric assay to determine the antioxidant capacity of several herbal extracts as in our previous work [29].…”

Section: Introductionmentioning

confidence: 99%

“…Here, the DPPH solution was immobilized as dry reagents [29] by a surface coating on the blister sheet as the sensing probes. Then, the sensor was set up as scanometric assay to determine the antioxidant capacity of several herbal extracts as in our previous work [29]. This is due to the fact that a pharmaceutical blister has thinner dimension than a microwell plate.…”

Section: Introductionmentioning

confidence: 99%

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Simple scanometric assay based on DPPH immobilized on pharmaceutical blister for determination of antioxidant capacity in the herbal extracts

Kuswandi¹,

Sari²,

Hidayat³

2018

jrp

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How to cite this article: Hidayat MA, Sari P, Kuswandi B. Simple scanometric assay based on DPPH immobilized on pharmaceutical blister for determination of antioxidant capacity in the herbal extracts. Marmara Pharm J. ABSTRACT: A simple scanometric assay for antioxidant capacity of the herbal extracts has been developed based on dry reagent of 2,2-diphenyl-1-picrylhydrazyl (DPPH) immobilized on the pharmaceutical blister. The deep violet color of DPPH solution was regenerated by introducing methanol to each well as sensing zones. The antioxidant capacity of plant extract will proportionally reduce the violet color to form the corresponding yellow color adduct, where the color change was captured by a flatbed scanner. Then, the optical responses toward antioxidant are presented as an averaged value of the red green blue (RGB) color using image color analysis (ImageJ). By using this color value, analytical performance of the antioxidant sensor could be characterized, where it has a linear range between 1-28 mg/L as gallic acid equivalent (GAE), with response time at 10 min. The reproducibility of the antioxidant sensor was good (RSD < 1%) with the recovery value of 102-105%. The scanometric assay was applied to several herbal extracts, such as sappan wood, guava leaf, and turmeric rhizome. The results obtained are in good agreement with the spectrophotometric method, suggesting that the proposed assay can be used as an alternative method for antioxidant capacity determination.

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Section: Application Of Antioxidant Sensor On the Plant Extractsmentioning

confidence: 99%

Section: Antioxidant Sensor Set-upmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Simple scanometric assay based on DPPH immobilized on pharmaceutical blister for determination of antioxidant capacity in the herbal extracts

Kuswandi¹,

Sari²,

Hidayat³

2018

jrp

Self Cite

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Purple to Yellow Silicone Elastomers: Design of a Versatile Sensor for Screening Antioxidant Activity

Ogliani

Skov

Brook

2019

Adv Materials Technologies

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Antioxidants play a key role in counteracting the adverse effects of oxidative stress in human organisms. Thus, there is a huge demand for the development of smart and convenient assays for the evaluation of antioxidant activity of synthetic and natural compounds, food samples, plant extracts, etc. The design of a solid-state, flexible, and portable format of the traditional in vitro 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical test is reported. For the first time, purple DPPH radicals are physically immobilized into a silicone matrix by means of a facile and rapid process. The working principle of the resulting sensor is based on the colorimetric process that is associated with the redox reaction of DPPH radicals with antioxidants. When an antioxidant reacts with the sensor, a color change from purple to yellow can be perceived by the naked eye. The response of the sensor is investigated qualitatively and quantitatively toward food samples and selected antioxidants of different natures, and solubilized in different media. It is demonstrated that the DPPH silicone sensor is highly versatile and can be used as a ready-to-use sensor for direct colorimetric detection of antioxidants.

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Evaluation of selected commercial pharmacotherapeutic drugs as potential pancreatic lipase inhibitors and antiproliferative compounds

Mamdooh

Kasabri

Al‐Hiari

et al. 2018

Drug Development Research

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In this study, 15 commercial acidic drugs have been evaluated for pancreatic lipase (PL) inhibitory activity using an in vitro spectrophotometric method. The acidity was the basis of selection, since most PL inhibitors exhibit acidic groups and high lipophilicity. Orlistat was the robust reference agent for potency and efficacy determinations. In comparison to the cisplatin, the evaluation of the antineoplastic activities of selected drugs in a panel of colorectal cancer cell lines (HT‐29, HCT‐116, SW620, CACO‐2, and SW480) and normal periodontal ligament fibroblasts for safety examination was performed by using a sulforhodamine procuring ascorbic acid as a reference in diphenyl‐2‐picryl‐hydrazyl assay of radical scavenging properties was performed. This research revealed three highly acidic pharmacological classes with reasonable PL inhibitory activity in comparison to orlistat out of 15 selected drugs, namely sulfonylureas, fluoroquinolones, and proton pump inhibitors. Docking studies supported the hypothesis of a selection based on acidity, since it showed that the sulfonamide acid group (glyburide) is a remarkably potent interacting group with the enzyme. Failing to fulfill other structure‐activity relationship requirements (lipophilicity) led to weak activity. Since the drugs are of different chemical classes with unknown mechanisms, they showed diverse antiproliferative activity. Some drugs such as atorvastatin and gemifloxacin showed higher antiproliferative activity than cisplatin with high‐safety profiles against SW620 and SW480 cell lines, respectively, whereas lansoprazole and clopidogrel revealed comparable activity to cisplatin against HCT‐116 and SW480, respectively. Unfortunately, selected tested drugs exhibited negligible radical scavenging activity versus ascorbic acid. Hit, Lead & Candidate Discovery

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Scanometry as microplate reader for high throughput method based on DPPH dry reagent for antioxidant assay

Cited by 33 publications

References 34 publications

Simple scanometric assay based on DPPH immobilized on pharmaceutical blister for determination of antioxidant capacity in the herbal extracts

Simple scanometric assay based on DPPH immobilized on pharmaceutical blister for determination of antioxidant capacity in the herbal extracts

Purple to Yellow Silicone Elastomers: Design of a Versatile Sensor for Screening Antioxidant Activity

Evaluation of selected commercial pharmacotherapeutic drugs as potential pancreatic lipase inhibitors and antiproliferative compounds

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