Scanning Ion Conductance Microscopy Reveals Differences in the Ionic Environments of Gram-Positive and Negative Bacteria

Cremin, Kelsey; Jones, Bryn A.; Teahan, James; Meloni, Gabriel N.; Perry, David; Zerfaß, Christian; Asally, Munehiro; Soyer, Orkun S.; Unwin, Patrick R.

doi:10.1021/acs.analchem.0c03653

Cited by 62 publications

(61 citation statements)

References 60 publications

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“…On one hand, the complex Gram-negative cell wall is made of multiple layers of peptidoglycans, lipoproteins, phospholipids, and lipopolysaccharides, which hampers the penetration of the CDs in bacterial cells. On the other side, the higher negative charge density present on Gram-positive bacteria compared to Gram-negative ones promotes the electrostatic interaction between CDs and the bacterial cells directing the CDs specificity toward Gram-positive species [ 93 ]. Collectively, these data demonstrate the potential of CDs as theranostic agents.…”

Section: Cds As Bacteria Targeting and Antibacterial Agentsmentioning

confidence: 99%

Carbon Dots as an Emergent Class of Antimicrobial Agents

2021

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Antimicrobial resistance is a recognized global challenge. Tools for bacterial detection can combat antimicrobial resistance by facilitating evidence-based antibiotic prescribing, thus avoiding their overprescription, which contributes to the spread of resistance. Unfortunately, traditional culture-based identification methods take at least a day, while emerging alternatives are limited by high cost and a requirement for skilled operators. Moreover, photodynamic inactivation of bacteria promoted by photosensitisers could be considered as one of the most promising strategies in the fight against multidrug resistance pathogens. In this context, carbon dots (CDs) have been identified as a promising class of photosensitiser nanomaterials for the specific detection and inactivation of different bacterial species. CDs possess exceptional and tuneable chemical and photoelectric properties that make them excellent candidates for antibacterial theranostic applications, such as great chemical stability, high water solubility, low toxicity and excellent biocompatibility. In this review, we will summarize the most recent advances on the use of CDs as antimicrobial agents, including the most commonly used methodologies for CD and CD/composites syntheses and their antibacterial properties in both in vitro and in vivo models developed in the last 3 years.

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Section: Cds As Bacteria Targeting and Antibacterial Agentsmentioning

confidence: 99%

Carbon Dots as an Emergent Class of Antimicrobial Agents

2021

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“…Dependent on metal species, the contribution of polysaccharides or proteins for binding of metal ions to EPS could be different [52] . Cellular surface substrates commonly have a negative charge in both cases of gram‐positive and gram‐negative bacteria [28,53] . Positively‐charged metal ions are easily stabilized when they are bound to the surface and locally supersaturated by electrostatic interactions [54,55] .…”

Section: Classifications Of Biomineralizationmentioning

confidence: 99%

Diversity of Microbial Metal Sulfide Biomineralization

Park

Faivre

2021

ChemPlusChem

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Since the emergence of life on Earth, microorganisms have contributed to biogeochemical cycles. Sulfate‐reducing bacteria are an example of widespread microorganisms that participate in the metal and sulfur cycles by biomineralization of biogenic metal sulfides. In this work, we review the microbial biomineralization of metal sulfide particles and summarize distinctive features from exemplary cases. We highlight that metal sulfide biomineralization is highly metal‐ and organism‐specific. The properties of metal sulfide biominerals depend on the degree of cellular control and on environmental factors, such as pH, temperature, and concentration of metals. Moreover, biogenic macromolecules, including peptides and proteins, help cells control their extracellular and intracellular environments that regulate biomineralization. Accordingly, metal sulfide biominerals exhibit unique features when compared to abiotic minerals or biominerals produced by dead cell debris.

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“…Recently, scanning ion conductance microscopy [ 1 , 16 , 17 , 18 ] and scanning photo-induced impedance microscopy [ 18 ] have been used for quantitative particle and cell surface charge mapping. This method employs a single nanopipette to measure cell topography and surface charge density based on the ion current rectification (ICR) principle [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Mapping Surface Charge Distribution of Single-Cell via Charged Nanoparticle

Ouyang

Shaik

et al. 2021

Cells

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Many bio-functions of cells can be regulated by their surface charge characteristics. Mapping surface charge density in a single cell’s surface is vital to advance the understanding of cell behaviors. This article demonstrates a method of cell surface charge mapping via electrostatic cell–nanoparticle (NP) interactions. Fluorescent nanoparticles (NPs) were used as the marker to investigate single cells’ surface charge distribution. The nanoparticles with opposite charges were electrostatically bonded to the cell surface; a stack of fluorescence distribution on a cell’s surface at a series of vertical distances was imaged and analyzed. By establishing a relationship between fluorescent light intensity and number of nanoparticles, cells’ surface charge distribution was quantified from the fluorescence distribution. Two types of cells, human umbilical vein endothelial cells (HUVECs) and HeLa cells, were tested. From the measured surface charge density of a group of single cells, the average zeta potentials of the two types of cells were obtained, which are in good agreement with the standard electrophoretic light scattering measurement. This method can be used for rapid surface charge mapping of single particles or cells, and can advance cell-surface-charge characterization applications in many biomedical fields.

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Scanning Ion Conductance Microscopy Reveals Differences in the Ionic Environments of Gram-Positive and Negative Bacteria

Cited by 62 publications

References 60 publications

Carbon Dots as an Emergent Class of Antimicrobial Agents

Carbon Dots as an Emergent Class of Antimicrobial Agents

Diversity of Microbial Metal Sulfide Biomineralization

Mapping Surface Charge Distribution of Single-Cell via Charged Nanoparticle

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