Scanning electron microscopy of the spermatheca in Sitophilus granarius (L.)

Tombes, Averett S.; Roppel, Richard M.

doi:10.1016/s0040-8166(71)80002-2

Cited by 16 publications

(3 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Besides that, in many insects the spermatheca receives and stores secretion from male accessory glands transferred during mating. Unfortunately, most of the data available in literature dealing with the spermatheca are related to systematics, while the ultrastructure of this organ has been studied in only 11 species belonging to the following ive orders: Dyctioptera (Gupta & Smith 1969), Orthoptera (Lay et al 1999), Diptera (Clements & Potter 1967, Filosi & Perotti 1975, Kokwaro et al 1981, Fritz & Turner 2002, Coleoptera (Happ & Happ 1970, Tombes & Roppel 1971, 1972, Villavaso 1975a, and Hymenoptera (Dallai 1975). The structure of this organ is very diversi ied among insects, varying from simple tube to complex organs (Matsuda 1976, Winterton et al 1999, Dallai et al 1996.…”

confidence: 99%

Ultrastructural and functional aspects of the spermatheca in the american harlequin bug, Murgantia histrionica (Hemiptera: Pentatomidae)

Stacconi¹,

Romani²

2011

Neotrop. entomol.

View full text Add to dashboard Cite

The spermatheca of Murgantia histrionica (Hahn) was investigated using luorescence, scanning and transmission electron microscopy. The aim of the study was to elucidate the structure of this organ, pointing out differences between mated and unmated females. Results have shown an elaborated cuticular structure associated with muscular and glandular tissues. The spermatheca is joined with the common oviduct by the spermathecal duct, forming a thin saccular dilation through two consecutive invaginations. The distal part of the organ is formed by a series of two communicating cuticular chambers. The irst cylindrical-shaped chamber, corresponding to the coiled region, is wrapped by longitudinal muscular ibers suspended between two cuticular langes. The contractions of these ibers compress a deformable zone of the cylinder, pumping the sperm toward the spermathecal duct. Without contractions the cylinder results to be isolated from the proximal part of the spermatheca by means of a valve. The second chamber, corresponding to the spermatheca, is made of two parts: a truncated-conical sub chamber, with a constant cuticular thickness, bearing on itself the distal lange, where muscular ibers are attached. The second part is a bulb-like structure wrapped in a glandular epithelium. The secretory units are composed by two cells: a secretory cell and an associated duct cell. Every evacuating duct shows a little reservoir just after the terminal apparatus, and converge inside the distal bulb after a tortuous path. The functional implications of this structure in the reproductive biology of M. histrionica are discussed.

show abstract

confidence: 99%

Ultrastructural and functional aspects of the spermatheca in the american harlequin bug, Murgantia histrionica (Hemiptera: Pentatomidae)

Stacconi¹,

Romani²

2011

Neotrop. entomol.

View full text Add to dashboard Cite

show abstract

“…The fine structure of the insect spermatheca andjor its associated gland has been examined in representatives of several orders including Coleoptera (Tenebrio molitor [6-8), Anthonomus grandis [9], Dytiscus marginalis [10], Phosphuga atrata [11), Sitophilus granarius [12,13]), Diptera (Aedes aegypti [14,15), Glossina austeni [16), Drosophila melanogaster [17]), Hymenoptera (Apis mellifera [18][19][20]), Dictyoptera (Periplaneta americana [4,21,22]), and Orthoptera (M. sanguinipes [present observations]). Taken collectively, these studies have demonstrated the remarkable uniformity of the basic secretory 'unit' (i.e., the secretory cell and associated cell(s)) within the spermatheca or its discrete gland (in Coleoptera and Hymenoptera).…”

Section: Discussionmentioning

confidence: 99%

The spermatheca ofMelanoplus sanguinipes(Fabr.). II. Ultrastructure

Ahmed

Gillott

1981

International Journal of Invertebrate Reproduction

View full text Add to dashboard Cite

The spermathecal wall of Melanoplus sanguinipes includes a thick intima. secretory units, a basal lamella, and small bundles of muscle. Each secretory unit consists of a secretory cell and an epithelial cell, the latter surrounding the former on all sides except that adjacent to the basal lamella. The secretory cell shows the ultrastructure typical of cells engaged in the synthesis of protein for export. Distally the cell is invaginated to form a central cavity lined with microvilli. Secretion is released into the central cavity and travels via the end apparatus and efferent ductule to the spermathecallumen. Basally, the secretory cell's plasma membrane is much folded and large numbers of mitochondria are concentrated in this region, along with small glycogen deposits, features perhaps related to the uptake of raw materials for manufacture of secretion. The epithelial cell flanges contain bundles of longitudinally arranged microtubules and large deposits of glycogen. Next to the basal lamella, the plasma membrane of the epithelial cell is greatly folded, like that of cells with an ionic regulatory function. spermatheca; ultrastructure; Me/anoplus sanguinipes

show abstract

“…Spermathecae morphology has been studied in several orders; Blattodea [22], Orthoptera [23], Coleoptera [24,25,26,27], Heteroptera [28,29,30,31], Hymenoptera [32,33], and Diptera [34,35,36,37,38,39] including D. melanogaster [17,18,19,21]. However, to the best of our knowledge, there is only one recent study on D. suzukii spermathecae morphology [40], and there are no studies on determining D. suzukii mating status.…”

Section: Introductionmentioning

confidence: 99%

Isolating Spermathecae and Determining Mating Status of Drosophila suzukii: A Protocol for Tissue Dissection and Its Applications

Avanesyan

Jaffe

Guédot

2017

Insects

View full text Add to dashboard Cite

The spotted wing drosophila, Drosophila suzukii (Diptera: Drosophilidae), is an emerging invasive pest, which attacks a wide variety of fruits and berries. Although previous studies have focused on different aspects of D. suzukii reproductive biology, there are no protocols available for determining the mating status of D. suzukii females and drosophilids in general. In this study, a step-by-step protocol for tissue dissection, isolating spermathecae, and determining the mating status of females was developed specifically for D. suzukii. This protocol is an effective and relatively quick method for determining female mating status. It has important applications from exploring reproductive output of D. suzukii females to understanding the biology of D. suzukii winter morph, which presumably plays the main role in the overwintering of this invasive species. We demonstrated applicability of this protocol for both field collected flies and flies reared in the lab, including fly specimens stored on a long-term basis.

show abstract

Scanning electron microscopy of the spermatheca in Sitophilus granarius (L.)

Cited by 16 publications

References 5 publications

Ultrastructural and functional aspects of the spermatheca in the american harlequin bug, Murgantia histrionica (Hemiptera: Pentatomidae)

Ultrastructural and functional aspects of the spermatheca in the american harlequin bug, Murgantia histrionica (Hemiptera: Pentatomidae)

The spermatheca ofMelanoplus sanguinipes(Fabr.). II. Ultrastructure

Isolating Spermathecae and Determining Mating Status of Drosophila suzukii: A Protocol for Tissue Dissection and Its Applications

Contact Info

Product

Resources

About