Isolating Spermathecae and Determining Mating Status of Drosophila suzukii: A Protocol for Tissue Dissection and Its Applications

Avanesyan, Alina; Jaffe, Benjamin D; Guédot, Christelle

doi:10.3390/insects8010032

Cited by 13 publications

(9 citation statements)

References 45 publications

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“…We also measured the size of the left spermatheca and visually determined whether it contained sperm. This latter observation allowed us to indirectly assess male reproductive development (Avanesyan et al, ).…”

Section: Methodsmentioning

confidence: 99%

Not berry hungry? Discovering the hidden food sources of a small fruit specialist, Drosophila suzukii

Stockton

Brown

Loeb

2019

Ecological Entomology

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1. Although polyphagy is widespread among Drosophila, some specialist species have evolved in response to resource competition and other selection factors favouring niche separation. The small fruit specialist Drosophila suzukii Matsumura has evolved a unique serrated ovipositor that allows it to access ripening fruit, a niche unavailable to most Drosophila. However, it is unclear whether ancestral traits (the use of non‐fruit resources) are maintained in this specialist species. 2. In this study, maternal preferences and offspring fitness in response to novel apple, mushroom, and bird manure‐based diets were investigated by comparing oviposition and offspring survival and development on various diets. The effect of those diets on survival at cool temperatures and the effect of natal environment/previous exposure on adult preferences were evaluated. 3. Female D. suzukii accepted non‐fruit diets such as mushroom and bird manure, and offspring completed their larval development on all diets tested. However, D. suzukii did not perform well on diets that included bird manure. By contrast, combinational apple/mushroom diets were associated with greater oviposition, lower mortality, faster development, and larger offspring than other diets. These diets were also associated with increased resistance to cold stress, and preference for these diets was positively affected by previous feeding experience. 4. These data suggest that D. suzukii may use non‐fruit resources when preferred resources are scarce. Given that this pest is adapted to temperate climates, alternative resources might provide seasonal nutritional support when fruit resources are not temporally available, although field data are needed to support this hypothesis.

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Section: Methodsmentioning

confidence: 99%

Not berry hungry? Discovering the hidden food sources of a small fruit specialist, Drosophila suzukii

Stockton

Brown

Loeb

2019

Ecological Entomology

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“…Females of many animal species store sperm after mating [ 48 ]. In Drosophila , females store sperm in specialized organs, spermatheca, which can contain the sperm from multiple males and can be stored for up to two weeks [ 47 ]. The sperm is transferred from the uterus to the spermathecal reservoir where it coils around the reservoir’s center, forming a toroidal mass that can be seen under a light microscope [ 47 ].…”

Section: Resultsmentioning

confidence: 99%

“…In Drosophila , females store sperm in specialized organs, spermatheca, which can contain the sperm from multiple males and can be stored for up to two weeks [ 47 ]. The sperm is transferred from the uterus to the spermathecal reservoir where it coils around the reservoir’s center, forming a toroidal mass that can be seen under a light microscope [ 47 ]. We therefore dissected the spermatheca of female Drosophila from unexposed and exposed mixed-sex groups to determine the presence of sperm ( Figure 8 ), which would indicate successful mating.…”

Section: Resultsmentioning

confidence: 99%

“…During mating in Drosophila , sperm is transferred from the uterus to the spermathecal reservoir where it is stored for up to two weeks, coiling around the reservoir’s center and forming a toroidal mass which can be easily detected under a light microscope [ 47 ]. Thus, we observed spermatheca at 400× total magnification to visually determine the presence of sperm, indicating successful mating.…”

Section: Methodsmentioning

confidence: 99%

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Toxicological Effects of Roundup® on Drosophila melanogaster Reproduction

Muller

Herrera

Talyn

et al. 2021

Toxics

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Herbicide use has increased dramatically since 2001, particularly Roundup®. Effective in agricultural practice, Roundup® adversely affects non-target organisms, including reproductive and endocrine systems. We exposed fruit flies, Drosophila melanogaster, to either Roundup® Ready to Use, containing pelargonic acid and glyphosate, or Roundup® Super Concentrate, that includes glyphosate and POEA, at sublethal concentrations. Both Roundup® formulations reduced ovary volume with fewer mature oocytes, most adversely at the highest concentration tested. Flies exposed within 2 h of eclosion were affected more than at 4 h, suggesting a critical period of increased ovarian sensitivity. These results support multi-species evidence that glyphosate-based herbicides interfere with normal development of the reproductive systems of non-target organisms.

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“…Mating status was determined by dissecting spermathecae under a microscope, using 40× objective lenses, following procedure proposed by | 791 KRÜGER Et al. Avanesyan et al (2017). For evaluation of mating status, a total of 30 females were evaluated, and females were considered as mated when presence of sperm mass was verified.…”

Section: Mating and Survival In Different Temperaturesmentioning

confidence: 99%

Effects of temperature and relative humidity on mating and survival of sterileDrosophila suzukii

Krüger

Vieira

Scheunemann

et al. 2021

J Applied Entomology

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Drosophila suzukii (Matsumura) (Diptera: Drosophilidae) is a widely distributed pest species of soft‐skinned fruits. Recent studies suggest the use of sterile insect technique (SIT) as a control method for this species; however, many factors can impact effectiveness of a SIT programme, including the environmental conditions. Environmental condition is critical at the time of the release and in the days afterwards, since it may impact sterile insects’ survival and ability to mate. Thus, we verified the influence of temperature and relative humidity on mating and survival of fertile and sterile D. suzukii, when insects were food provided or deprived. Highest mating rates occurred when sterile or fertile flies provided with food were exposed to 25ºC or 81%–100% relative humidity, while temperatures of 10 and 35ºC and humidity below 60% impaired mating. Overall, mating rate among food‐deprived flies was low in all temperatures and humidity levels tested, but fertile insects were more prone to mate when compared to sterile flies. Survival was negatively influenced by high temperatures, low relative humidity and food deprivation. The information present in this study is useful to be considered for release of sterile D. suzukii.

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Isolating Spermathecae and Determining Mating Status of Drosophila suzukii: A Protocol for Tissue Dissection and Its Applications

Cited by 13 publications

References 45 publications

Not berry hungry? Discovering the hidden food sources of a small fruit specialist, Drosophila suzukii

Not berry hungry? Discovering the hidden food sources of a small fruit specialist, Drosophila suzukii

Toxicological Effects of Roundup® on Drosophila melanogaster Reproduction

Effects of temperature and relative humidity on mating and survival of sterileDrosophila suzukii

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