Scanning electron microscopy of human dental pulp

Gotjamanos, T.; Swedlow, David B.

doi:10.1016/0003-9969(74)90070-3

Cited by 15 publications

(2 citation statements)

References 10 publications

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“…It has previously been reported that odontoblasts remain on the surface of predentin after pulp extraction in human (Gotjamanos and Swedlow, 1974), bovine (Munksgaard and Moe, 1980;Oida et al, 1982), and rat teeth (Munksgaard et al, 1978). We also observed, using light microscopy, the retention of odontoblasts in pig teeth on the surface of predentin following the removal of the dental pulp (Fig.…”

Section: Discussionsupporting

confidence: 73%

Amelogenin Gene Expression in Porcine Odontoblasts

et al. 2002

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Amelogenin is the major organic component in the enamel matrix of developing teeth and plays an important role in enamel biomineralization. Amelogenin has been reported to be a specific secretory product of ameloblasts. In this study, we examined amelogenin gene expression in various cell layers prepared from a porcine permanent tooth germ using reverse transcription-polymerase chain-reaction (RT-PCR). Amelogenin amplification products were detected only in the secretory ameloblast layer after 20 cycles of PCR. After 30 cycles of PCR, amelogenin amplification products were detected in secretory and maturation-stage ameloblasts and in odontoblasts. The relative levels of amelogenin gene expression in secretory and maturation-stage ameloblasts and odontoblasts were determined. Secretory ameloblasts expressed over 1000 times the level of amelogenin mRNA found in odontoblasts. Amelogenin gene expression in odontoblasts was confirmed in an erupted porcine permanent first molar, which has no ameloblasts. Amelogenin PCR amplification products were identified from 4 different alternatively spliced transcripts in the ameloblast samples, and the same spliced forms were detected in the odontoblast samples.

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Section: Discussionsupporting

confidence: 73%

Amelogenin Gene Expression in Porcine Odontoblasts

et al. 2002

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“…The discovery that a single layer of odontoblasts retoains attached to predentin in utifixed specimens when the pulp tissue is forcefully stripped away is relatively old (25), but seldomly mentioned, although taken advantage of by other authors also (26).…”

Section: Discussionmentioning

confidence: 99%

High resolution scanning electron microscopy of the subplasmalemmal cytoskeleton in human odontoblasts

Sögaard-Pedersen

Boye

1990

European J Oral Sciences

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– The subplasmalemmal cytoskeleton in human odontoblasts was studied by high resolution scanning electron microscopy. The odontoblast layer was isolated and exposed to formaldehyde, glutaraldehyde, and OsO4 for some specimens, while the membraneous structures and soluble proteins in the dental tissue were removed by Zenker's solution and 1% OsO4 for other specimens, without further fixation of the remaining components. The cytoskeletal elements comprised a dense network of interlacing filaments of different diameters in the cell body. Most cytoskeletal elements were parallel to the axis of the cell processes situated inside the dentinal tubules. The appearance and orientation of the investigated subplasmalemmal cytoskeletal elements was unaffected by the choice of method. Both methods confirm the presence of a subplasmalemmal cytoskeleton in human odontoblasts.

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Scanning electron microscopic studies of the rat incisor odontoblastema

1975

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A scanning electron microscopic technique was used to investigate the surface structure of dentinogenically active odontoblasts. Thin pieces of rat incisors were fixed, rapidly frozen, freezedried at minus 70 degrees C and fractured to expose new surfaces prior to examination in the SEM. Differences in the appearance of odontoblastic cell surfaces were seen, with the most extensive ridge formations at the distal part of the sides of the odontoblasts. The predentine area displayed a spongy structure which contrasted to the compact appearance of dentine. Results are discussed in relation to previous studies at the light microscopic and transmission electron miscroscopic levels.

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Scanning electron microscopy of human dental pulp

Cited by 15 publications

References 10 publications

Amelogenin Gene Expression in Porcine Odontoblasts

Amelogenin Gene Expression in Porcine Odontoblasts

High resolution scanning electron microscopy of the subplasmalemmal cytoskeleton in human odontoblasts

Scanning electron microscopic studies of the rat incisor odontoblastema

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