High resolution scanning electron microscopy of the subplasmalemmal cytoskeleton in human odontoblasts

Sögaard-Pedersen, Bo; Boye, Hans

doi:10.1111/j.1600-0722.1990.tb00987.x

European J Oral Sciences

1990

DOI: 10.1111/j.1600-0722.1990.tb00987.x

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High resolution scanning electron microscopy of the subplasmalemmal cytoskeleton in human odontoblasts

Bo Sögaard-Pedersen

Hans Boye

Abstract: – The subplasmalemmal cytoskeleton in human odontoblasts was studied by high resolution scanning electron microscopy. The odontoblast layer was isolated and exposed to formaldehyde, glutaraldehyde, and OsO4 for some specimens, while the membraneous structures and soluble proteins in the dental tissue were removed by Zenker's solution and 1% OsO4 for other specimens, without further fixation of the remaining components. The cytoskeletal elements comprised a dense network of interlacing filaments of different d… Show more

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Cited by 2 publications

References 18 publications

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Scanning Electron Microscopy of the Organelles of Rat Incisor Odontoblasts

Nishimura¹,

Iwai-Liao²

1994

Okajimas Folia Anatomica Japonica

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The fine structure of rat odontoblasts (OBs) showing the developmental sequence of cellular maturation along the long axis of the incisor was studied. The cytomorphological changes and relationships of the intracellular components, particularly the membranous component, were investigated mainly by scanning electron microscopy (SEM) using the AODO (A: aldehyde prefixation; O: osmium tetroxide postfixation; D: dimethyl sulfoxide (DMSO) freeze-fracture; O: osmium tetroxide maceration) method and transmission electron microscopy (TEM) using the conventional methods. The following results were obtained: 1) The elongated young OBs were highly polarized and characterized by well-developed concentric cisternal granular (rough) endoplasmic reticulum (rER) layers and Golgi complex. SEM showed the rER was continuous to the agranular (smooth) ER (sER). 2) Transformation of the concentric rER to a tubular network was closely related to the maturation and secretory activity of the OBs. 3) Many branched and elongated tubular mitochondria were observed in the supranuclear region of young OBs. On the other hand, many round-to-ovoid mitochondria were observed particularly in the distal clear area of old OBs. 4) SEM also revealed tubulo-vesicular elements, which are believed to be structures relating to secretory and absorptive functions, composed of secretory granules and small tubules, having connections with the cell membrane in both young and old OBs. Furthermore, the small tubules were continuous with the larger sER tubules in the distal clear area and cell process of the OBs. 5) Using TEM, long lysosomes, various cytosomes and multivesicular bodies (MVBs) were usually observed in the peripheral region and in the distal cytoplasm and major processes of the OBs. The present SEM and TEM studies indicated the MVBs were closely associated with sER tubules of the tubulo-vesicular elements. 6) Both TEM and SEM showed cytosegresomes containing laminated ER and other organelles particularly in the distal clear area of old OBs. 7) SEM also showed some thin lateral branchings of the OBs running along the fine matricial fibrils, as well as matrix-vesicle-like structures deeply invaginated at the basal portion of the cell processes and distributed in the predentine. The present morphological study demonstrated a three-dimensional configuration of the structures relating to secretory and absorptive functions of young and old OBs.

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Scanning Electron Microscopy of the Organelles of Rat Incisor Odontoblasts

Nishimura¹,

Iwai-Liao²

1994

Okajimas Folia Anatomica Japonica

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Observation of the Internal Configuration of Rat Incisor Odontoblasts by Scanning Electron Microscopy Using the AODO Method

Iwai-Liao¹,

Higashi²,

Sankouji³

et al. 1995

Okajimas Folia Anatomica Japonica

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Key Words: Internal structures, Rat incisor odontoblasts, Scanning electron microscopy (SEM) using the aldehyde prefixation-osmium tetroxide postfixation -DMSO freeze fracture -Osmium teroxide maceration (AODO) method Summary: The internal configuration of rat incisor odontoblasts was studied mainly by scanning electron microscopy (SEM) using the AODO method (low concentration aldehyde prefixation, osmium tetroxide postfixation, dimethyl sulfoxide (DMSO) freeze-fracture, osmium tetroxide maceration). The present SEM findings were compared with the results obtained by conventional transmission electron microscopy (TEM) of epon-embedded specimens. The following results were obtained: 1) Functioning odontoblasts were characterized by a concentric, laminar rough endoplasmic reticulum (rER) with many long mitochondria interposed. 2) A network of tubular smooth endoplasmic reticulum (sER) was observed in the odontoblast process and distal portion of both functioning and resting odontoblasts.3) The tubulo-vesicular elements which have been found to present a modified Golgi-GERL organelle with secretory and absorptive functions were demonstrated in both the functioning and resting odontoblasts. Structurally they consist of the sER network and strings of granules and vesicles. 4) Various types of cytoplasmic bodies, e.g., lysosomes, cytosomes and multivesicular bodies, related to the sER were also noted in both functioning and resting odontoblasts. 5) Microapocrine secretion of membranous vesicles of varous sizes into the predentin and along the lateral branchings of odontobalst processes in the circumpulpal dentin was observed during the matrix apposition stage of the odontoblasts. The present morphological study revealed the threedimensional configuration of the intra-and extra-cellular structures related to dentinogenesis by odontoblasts.There have been many transmission electron microscopy (TEM) studies on the morphological changes and the fine structure related to secretion and absorption by odontoblasts (OBs) at different developmental stages

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High resolution scanning electron microscopy of the subplasmalemmal cytoskeleton in human odontoblasts

Cited by 2 publications

References 18 publications

Scanning Electron Microscopy of the Organelles of Rat Incisor Odontoblasts

Scanning Electron Microscopy of the Organelles of Rat Incisor Odontoblasts

Observation of the Internal Configuration of Rat Incisor Odontoblasts by Scanning Electron Microscopy Using the AODO Method

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