Scanning electron microscopy of early infection structure formation by Puccinia recondita f. sp. tritici on and in susceptible and resistant wheat lines

Hu, Guanggan; Rijkenberg, F. H. J.

doi:10.1017/s0953756297005054

Cited by 54 publications

(49 citation statements)

References 24 publications

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“…Several genes associated with photosynthesis, oxidative reactions, signal transduction, ubiquitination and precursors to the shikimate-phenylpropanoid pathway showed differential expression between the compatible and incompatible interactions and appeared to represent key genes in the metabolism of defense [35]. The time-points coincided with the intracellular growth of the fungus and the formation of the septum that separates the infective hyphae from the newly-formed primary haustorial mother cell, which had been described in a previous study [36].…”

Section: Microarrayssupporting

confidence: 60%

Gene Expression: A Review on Methods for the Study of Defense-Related Gene Differential Expression in Plants

Casassola¹,

Brammer²,

Chaves³

et al. 2013

AJPS

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The plant genes involved in cellular signaling and metabolism have not been fully identified, while the function(s) of many of those which have are as yet incompletely characterized. Gene expression analysis allows the identification of genes and the study of their relationship with cellular processes. There are several options available for studying gene expression, including the use of cDNA and microarray libraries and techniques such as suppression subtractive hybridization (SSH), differential display (DD), RNA fingerprinting by arbitrary primed PCR (RAP), expressed sequence tags (EST), serial analysis of gene expression (SAGE), representational difference analysis (RDA), cDNA-amplified fragment length polymorphism (cDNA-AFLP) and RNA sequencing (RNA-Seq). Focusing on defense-related processes in plants, we present a brief review and examples of each of these methodologies and their advantages and limitations regarding the study of plant gene expression.

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Section: Microarrayssupporting

confidence: 60%

Gene Expression: A Review on Methods for the Study of Defense-Related Gene Differential Expression in Plants

Casassola¹,

Brammer²,

Chaves³

et al. 2013

AJPS

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“…The susceptible clone already had many urediniospores germinated with appressorium followed by cuticle and wax degradation. Different to other Puccinia species, in that penetration occurs via stomata [21][22][23], the penetration of A. psidii occurs between the anticlinal walls of the leaf epidermis directly into the mesophyll of the leaf after appressoria formation [24]. Once formed, appressoria adhere tightly to the leaf surface and secrete extracellular enzymes, or generate physical force, or use a combination of both factors to bring about cuticle penetration [25].…”

Section: Discussionmentioning

confidence: 99%

Pre-Infection Stages of Austropuccinia psidii in the Epidermis of Eucalyptus Hybrid Leaves with Different Resistance Levels

Silva

Rodella

et al. 2017

Forests

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Rust is a major Eucalyptus spp. disease, which is especially damaging for early-stage plants. The aim of this study was to verify the pre-infection process of Austropuccinia psidii (A. psidii) in the leaves of three phenological stages of Eucalyptus clones with different resistance levels. Plants from the hybrids of Eucalyptus urophylla × Eucalyptus grandis (E. grandis) with variable levels of resistance to this disease were used. The pathogen was inoculated in vitro on abaxial leaf discs of first, third, and fifth leaf stages and maintained under conditions suitable for disease development. Subsequently, samples from these discs were collected 24 and 120 h after inoculation and processed using scanning electron microscopy analysis. No symptoms were seen in any leaf stage of the resistant clone. Additionally, a low incidence of A. psidii germination (1.3-2%) and appressoria (0-0.5%) in three leaf stages was observed. However, the first leaf stage of the susceptible clone presented germination of large numbers of urediniospores (65%) with appressoria (55%) and degradation of the cuticle and wax. From the third stage, the percentage of germinated urediniospores (<15%) and appressoria (<2%) formation of this clone decreased. Protrusions on the leaf surface, associated with the pathogen, were observed on the first and third leaf stages of the resistant clone and on the fifth stage of the susceptible clone, suggesting a possible defensive plant reaction.

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“…Tecidos vegetais são compostos por células as quais são constituídas por parede celular e vacúolos que possuem moléculas como ácidos orgânicos, carboidratos, lipídios e fenóis. Portanto, a etapa de extração requer maior atenção, visto que essas moléculas podem interferir na análise proteômica (33).…”

Section: Principais Genes E Seus Mecanismos De Resistência Em Cereaisunclassified

Mecanismos de defesa do trigo contra a ferrugem da folha por genes e proteínas

et al. 2017

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O agente causal da ferrugem da folha do trigo é o fungo Puccinia triticina. Essa doença causa danos elevados que podem comprometer a produtividade da cultura do trigo em até 80%, quando a infecção é intensa antes do florescimento e do enchimento de grãos. A utilização de cultivares resistentes é a melhor estratégia de controle da ferrugem da folha. Porém, devido à variabilidade do patógeno, a resistência dos genótipos é superada em até três anos após o seu lançamento. Para se defender da infecção do patógeno a planta desencadeia Finger, G.; Heckler, L.I.; Silva, G.B.P.; Chaves, M.S.; Martinelli, J.A. Mecanismos de defesa do trigo contra a ferrugem da folha por genes e proteínas. Summa Phytopathologica, v.43, n.4, p.354-358, 2017. Palavras-chave:Triticum aestivum, Puccinia triticina, proteômica, resistência, infecção. RESUMOmecanismos de defesa, os quais têm a finalidade de evitar que o fungo colonize os tecidos do hospedeiro. Esses mecanismos de defesa podem estar associados com a expressão de genes que possuem a função de codificar proteínas envolvidas na resistência. Esta revisão discute a importância da interação plantapatógeno bem como das proteínas envolvidas. Também apresenta as principais técnicas de proteômica que visam identificar e quantificar as diferentes proteínas expressas nas células vegetais. ABSTRACTThe fungus Puccinia triticina is the causal agent of wheat leaf rust. This disease causes great damages, which may compromise wheat crop yields by up to 80% when the infection is intense before flowering and grain filling. The use of resistant cultivars is the best strategy to control leaf rust. However, due to the pathogen variability, genotype resistance is easily overcome within three years under field conditions. To defend against the pathogen infection, the plant triggers defense mechanisms, which aim to prevent the fungus from colonizing the host tissues. Such defense mechanisms may be associated with the expression of genes that have a role in encoding proteins involved in resistance. In this review, the importance of both the plant-pathogen interaction and the role of involved proteins is discussed. The main proteomic techniques to identify and quantify the different proteins expressed in plant cells are also presented. Triticum aestivum, Puccinia triticina, proteomics, resistance, infection. A cultura do trigo (Triticum aestivum L.) possui grande importância no setor econômico mundial. Dentre os cereais mais produzidos no mundo, ocupa a terceira posição, superado apenas pelo milho e o arroz. Considerado a segunda cultura cerealífera, o trigo tem grande importância para a alimentação humana e está entre as primeiras culturas domesticadas (26). O grão de trigo é rico em nutrientes essenciais para o homem, possuindo em torno de 60 a 80% de carboidratos, vitaminas B e E, e proteínas (3). Cerca de 30% da população mundial encontra no cereal, e em seus derivados, a fonte primária de energia para sua dieta (15). Keywords:Os principais países produtores de trigo são China, União Européia, Estados...

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Scanning electron microscopy of early infection structure formation by Puccinia recondita f. sp. tritici on and in susceptible and resistant wheat lines

Cited by 54 publications

References 24 publications

Gene Expression: A Review on Methods for the Study of Defense-Related Gene Differential Expression in Plants

Gene Expression: A Review on Methods for the Study of Defense-Related Gene Differential Expression in Plants

Pre-Infection Stages of Austropuccinia psidii in the Epidermis of Eucalyptus Hybrid Leaves with Different Resistance Levels

Mecanismos de defesa do trigo contra a ferrugem da folha por genes e proteínas

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