Scanning  Electron  Microscopy  in  modern  dentistry  research

Paradella, Thaís Cachuté; Bottıno, Marco Antônıo

doi:10.14295/bds.2012.v15i2.798

Cited by 25 publications

(13 citation statements)

References 18 publications

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“…In addition, since SEM figures are in gray scale, the color of dentin does not influence in obtaining a correct focus, a limitation which is found in optical stereomicroscopes. 14 …”

Section: Discussionmentioning

confidence: 99%

Efficacy of Caries Removal by Carie-Care and Erbiumdoped Yttrium Aluminum Garnet Laser in Primary Molars: A Scanning Electron Microscope Study

Prabhakar

Lokeshwari

Naik

et al. 2018

International Journal of Clinical Pediatric Dentistry

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AimTo compare and evaluate morphological changes and bacterial deposits in primary carious molars after caries excavation with Carie-Care, erbium-doped yttrium aluminum garnet (Er:YAG) laser, and round tungsten carbide bur.Materials and methodsThirty human carious primary molars extracted for therapeutic reasons were sectioned mesiodistally. These sectioned samples were allocated into three groups (20 samples each): group I: Carie Care, group II: Er:YAG laser, and group III: round tungsten carbide bur. After caries excavation, all samples were processed and examined under conventional light microscope to examine for bacterial deposits. Representative samples from each group were processed and analyzed to examine the morphology of caries-excavated tissue by scanning electron microscope (SEM). Statistical analysis was done using Fisher’s exact test, Kruskal–Wallis test, and Mann-Whitney U test.ResultsThe Er:YAG laser showed best results with no smear layer followed by chemomechanically excavated surfaces with Carie-Care. Amount of bacterial deposits was observed to be more in group I while least in group II (p-value < 0.001). Mann-Whitney U test and Fisher’s exact test revealed that there was statistically significant difference among all the three groups.ConclusionAmong the three different methods of caries excavation, Er:YAG laser was found to be more effective compared with Carie-Care and round tungsten carbide bur.Clinical significanceLaser-induced caries excavation by Er:YAG laser and chemomechanical method of caries removal by Carie-Care can be considered as future of noninvasive pediatric and preventive dentistry.How to cite this article: Prabhakar A, Lokeshwari M, Naik SV, Yavagal C. Efficacy of Caries Removal by Carie-Care and Erbium-doped Yttrium Aluminum Garnet Laser in Primary Molars: A Scanning Electron Microscope Study. Int J Clin Pediatr Dent 2018;11(4):323-329.

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“…In addition, since SEM figures are in gray scale, the color of dentin does not influence in obtaining a correct focus, a limitation which is found in optical stereomicroscopes. 14 …”

Section: Discussionmentioning

confidence: 99%

Efficacy of Caries Removal by Carie-Care and Erbiumdoped Yttrium Aluminum Garnet Laser in Primary Molars: A Scanning Electron Microscope Study

Prabhakar

Lokeshwari

Naik

et al. 2018

International Journal of Clinical Pediatric Dentistry

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“…Nevertheless, these factors need to be considered when selecting the site of analysis; however, the included studies lacked this detail and only described images being taken in ‘representative areas’ or ‘areas of interest’ which could potentially introduce bias. Additionally, 14 different microscopes were used with varying magnifications making it difficult to interpret results between studies (Paradella & Bottino ).…”

Section: Limitationsmentioning

confidence: 99%

Efficacy of irrigant activation techniques in removing intracanal smear layer and debris from mature permanent teeth: a systematic review and meta‐analysis

et al. 2017

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Virdee SS, Seymour DW, Farnell D, Bhamra G, Bhakta S. Efficacy of irrigant activation techniques in removing intracanal smear layer and debris from mature permanent teeth: a systematic review and meta-analysis. International Endodontic Journal.Aims To establish whether irrigant activation techniques (IATs) result in greater intracanal smear layer and debris removal than conventional needle irrigation (CNI). Methodology Six electronic databases were searched to identify scanning electron microscopy studies evaluating smear layer and/or debris removal following the use of manual dynamic activation (MDA), passive ultrasonic irrigation (PUI), sonic irrigation (SI) or apical negative pressure (ANP) IATs in mature permanent teeth. Meta-analyses were performed for each canal segment (coronal, middle, apical and apical 1 mm) in addition to subgroup analyses for individual IATs with respect to CNI. Outcomes were presented as standardized mean differences (SMD) alongside 95% confidence intervals (95% CI) and chi-squared analysis.Results From 252 citations, 16 studies were identified. The meta-analyses demonstrated significant improvements in coronal (SMD: 1.15, 95% CI: 0.72-1.57 / SMD: 0.54, 95% CI: 0.29-0.80), middle (SMD: 1.30, 95% CI: 0.59-2.53 / SMD: 0.8, 95% CI: 0.58-1.13) and apical thirds (SMD: 1.22, 95% CI: 0.83-1.62 / SMD: 1.86, 95% CI: 0.76-2.96) for smear layer and debris removal, respectively. In the apical 1 mm IATs improved cleanliness; however, differences were insignificant (SMD: 1.15, 95% CI: -0.47-2.77). Chi-squared analysis revealed heterogeneity scores of 79.3-92.8% and 0.0-93.5% for smear layer and debris removal, respectively. Conclusions IATs improve intracanal cleanliness across a substantial portion of the canal, and therefore, their use is recommended throughout root canal preparation. However, current data is too heterogeneous to compare and identify superiority of an individual technique highlighting the need to standardize experimental protocols and develop a more representative research model to investigate the in vivo impact of IATs on clinical outcomes and periapical healing following root canal treatment.

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“…12 SEM visualizes the adaptation of restoration to cavity margins because of its good depth of focus and higher magnification. 13 It also allows better visualization of irregular root-dentin structures, with different heights, without altering the focus, and generates data in digital form. 14 Henceforth, this study was planned for evaluating the gaps at the margins MTA, Biodentine, and MTA Plus when used as root-end filling materials using SEM.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Marginal Adaptation of MTA, Biodentine, and MTA Plus as Root-End Filling Materials—An SEM Study

Bansal

Matta

et al. 2019

Dental Journal of Advance Studies

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Objective This study aimed to evaluate adaptation of mineral trioxide aggregate (MTA), Biodentine, and MTA Plus when used as root-end restorative materials using scanning electron microscope. Materials and Methods Forty-five extracted teeth with one root canal were cleaned and sectioned at cementoenamel junction, maintaining a standardized length of 15 mm. Chemomechanical preparation and obturation was done in all the samples, and access was restored with glass ionomer cement (GIC). Root-end cavities were prepared in all the samples after 3-mm apical root end was resected. Forty-five samples were divided into three groups with 15 samples each. Root apices were filled with ProRoot MTA in group I, Biodentine in group II, and MTA Plus in group III. The sample teeth were sectioned for examination of gaps at the margins using scanning electron microscope. The data collected were put to statistical analysis by one-way ANOVA (analysis of variance) and post hoc tests. Results The minimum margin gap score of 2.44 μm was observed in group II (Biodentine), whereas maximum gap score of 5.63 μm was observed in group I (ProRoot MTA); 4.92 μm mean margin gap score was observed in group III (MTA Plus). Conclusion Biodentine showed least margin gap at the interface, so it can be considered as the best root-end filling material, whereas, MTA Plus can be considered as a substitute to ProRoot MTA as a root-end filling material.

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Scanning Electron Microscopy in modern dentistry research

Cited by 25 publications

References 18 publications

Efficacy of Caries Removal by Carie-Care and Erbiumdoped Yttrium Aluminum Garnet Laser in Primary Molars: A Scanning Electron Microscope Study

Efficacy of Caries Removal by Carie-Care and Erbiumdoped Yttrium Aluminum Garnet Laser in Primary Molars: A Scanning Electron Microscope Study

Efficacy of irrigant activation techniques in removing intracanal smear layer and debris from mature permanent teeth: a systematic review and meta‐analysis

Evaluation of Marginal Adaptation of MTA, Biodentine, and MTA Plus as Root-End Filling Materials—An SEM Study

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