Scanning electron microscopical observation of an osteoblast/osteoclast co-culture on micropatterned orthopaedic ceramics

Halai, Mansur; Ker, Andrew; Meek, R.M.D.; Nadeem, Danish; Sjöström, Terje; Su, Bo; McNamara, Laura E.; Dalby, Matthew J.; Young, P.S.

doi:10.1177/2041731414552114

Cited by 15 publications

(9 citation statements)

References 31 publications

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“…Further studies reported the use of porcine co-cultures of bone marrow stromal cells (BMSCs) and bone marrow haematopoetic cells (BMHCs)16, or of human co-cultures of BMSC and CD34 + BMHC17 or MSC and PBMCs18. We recently developed a methodology to allow mesenchymal bone marrow stromal cell (BMSC)/bone marrow haematopoetic cell (BMHC) co-cultures that could form mature osteoblasts and osteoclasts in culture in response to materials1920. Further, we have illustrated that nanoscale topography in polymers19 and in titanium can present positive osteogenic cues while osteoclastogenesis remains unchanged; i.e.…”

mentioning

confidence: 99%

Osteogenic and bactericidal surfaces from hydrothermal titania nanowires on titanium substrates

Tsimbouri

Fisher

Holloway

et al. 2016

Sci Rep

102

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Nanotopographical cues on Ti have been shown to elicit different cell responses such as cell differentiation and selective growth. Bone remodelling is a constant process requiring specific cues for optimal bone growth and implant fixation. Moreover, biofilm formation and the resulting infection on surgical implants is a major issue. Our aim is to identify nanopatterns on Ti surfaces that would be optimal for both bone remodelling and for reducing risk of bacterial infection. Primary human osteoblast/osteoclast co-cultures were seeded onto Ti substrates with TiO2 nanowires grown under alkaline conditions at 240 °C for different times (2, 2.5 or 3 h). Cell growth and behaviour was assessed by scanning electron microscopy (SEM), immunofluorescence microscopy, histochemistry and quantitative RT-PCR methods. Bacterial colonisation of the nanowire surfaces was also assessed by confocal microscopy and SEM. From the three surfaces tested the 2 h nanowire surface supported osteoblast and to a lesser extent osteoclast growth and differentiation. At the same time bacterial viability was reduced. Hence the 2 h surface provided optimal bone remodeling in vitro conditions while reducing infection risk, making it a favourable candidate for future implant surfaces.

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mentioning

confidence: 99%

Osteogenic and bactericidal surfaces from hydrothermal titania nanowires on titanium substrates

Tsimbouri

Fisher

Holloway

et al. 2016

Sci Rep

102

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“…Osteoblasts are derived from mesenchymal stromal cells, which are responsible for bone matrix synthesis and subsequent mineralization (12). Chronic persistent inflammation inhibits bone formation and osteoblast differentiation.…”

Section: Introductionmentioning

confidence: 99%

Triptolide inhibits the function of TNF-α in osteoblast differentiation by inhibiting the NF-κB signaling pathway

Liu

Wang

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. Chronic inflammation often delays fracture healing or leads to bone nonunion. Effectively suppressing pathological inflammation is crucial for fracture healing or bone remodeling. Triptolide, which is a diterpenoid epoxide, is the major active component of the Thunder God Vine, Tripterygium wilfordii. The aim of the present study was to investigate the role of triptolide in osteoblast differentiation and explore the molecular mechanisms of triptolide in fracture healing. Alkaline phosphatase (ALP) activity was used to evaluate osteoblast differentiation. ALP activity was measured via histochemical staining and western blotting was used to determine the expression of factors associated with inflammation. C2C12 cells were initially treated with 200 ng/ml bone morphogenetic protein (BMP)-2 alone for 3 days, which caused a significant increase in ALP activity (P<0.01). However, treatment with tumor necrosis factor (TNF)-α significantly decreased the ALP activity (P<0.05). Notably, treatment with the chronic inflammatory cytokine TNF-α significantly decreased the effect of BMP-2 in C2C12 cells compared with BMP-2 treatment alone (P<0.01). C2C12 cells were treated with increasing concentrations of BMP-2 or TNF-α for 3 days. The results demonstrated that TNF-α treatment significantly inhibited BMP-2-induced osteoblast differentiation in a dose-dependent manner (P<0.01). The role of triptolide in BMP-2-induced osteoblast differentiation was also examined. Cells were treated with BMP-2, BMP-2 + TNF-α alone, or BMP2 + TNF-α with increasing concentrations of triptolide (4, 8 or 16 ng/ml). After 3 days, the results of ALP activity revealed that triptolide significantly reversed the TNF-α-associated inhibition of osteoblast differentiation (P<0.01). Western blotting analysis demonstrated that triptolide markedly inhibited the phosphorylation of nuclear factor-κB, therefore suppressing the effects of TNF-α. In summary, triptolide is able to reverse the TNF-α-associated suppression of osteoblast differentiation, suggesting that triptolide treatment may have a positive effect on bone remodeling and fracture repairing.

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“…In this study, a surface roughness of the zirconia disk did not show any difference with that of the Ti disk. Considering that the surface topography of materials contributed to differences in osteoclast behavior in previous reports [ 37 , 38 , 39 ], the surface roughness did not affect cellular responses in a comparison between the zirconia and Ti surfaces. We also showed that zirconia and Ti disks were pure and contained no contaminants using EDS.…”

Section: Discussionmentioning

confidence: 69%

Osteoclastogenesis Behavior of Zirconia for Dental Implant

et al. 2019

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Zirconia is worth studying as an alternative to dental titanium implants to overcome the disadvantages of titanium. This study investigated the surface characteristics of the zirconia implant material and osteoclastogenesis responses on the surface compared with titanium. Yttrium oxide-stabilized 5% tetragonal zirconia polycrystalline specimens were manufactured, and osteoclast-precursor cells were cultured and differentiated into osteoclasts on the specimens. Surface shape, roughness, and chemical composition were evaluated. After culturing, cell morphologies and differentiation capacity were analyzed using tartrate-resistant acid phosphatase activity (TRACP). mRNA of two critical transcription factors, nuclear factor of activated T-cells 1 (NFATc1) and c-Fos were measured, and protein levels of NFATc1 and c-Fos were investigated. The zirconia specimens had rhomboid-like shapes with smooth surfaces and exhibited no difference in surface roughness compared to the titanium specimens. Morphologies of differentiated osteoclasts on both materials were similar. TRACP activity on the zirconia showed comparable results to that on the titanium. The mRNA value of NFATc1 on the zirconia was higher than that on the titanium at day four. The protein level of c-Fos was expressed thicker on the zirconia when compared to the titanium at day two. The results of this study suggest that zirconia material provides adequate osteoclastogenesis behaviors for dental implant use.

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Scanning electron microscopical observation of an osteoblast/osteoclast co-culture on micropatterned orthopaedic ceramics

Cited by 15 publications

References 31 publications

Osteogenic and bactericidal surfaces from hydrothermal titania nanowires on titanium substrates

Osteogenic and bactericidal surfaces from hydrothermal titania nanowires on titanium substrates

Triptolide inhibits the function of TNF-α in osteoblast differentiation by inhibiting the NF-κB signaling pathway

Osteoclastogenesis Behavior of Zirconia for Dental Implant

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