Scanning electron microscopic studies of the hyaloid vascular system in newborn mice exposed to O2 and CO2

Bischoff, Peter; Wajer, Stephen D.; Flower, Robert W.

doi:10.1007/bf00231352

Cited by 21 publications

(11 citation statements)

References 6 publications

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“…Some authors assert that the new vessels are derived not only from the hyaloid system, but also from the retinal vessels,zo.zl while others assert that true intravitreal vasoproliferation does not take place. 22 Our observation of intravitreal new vessels derived from the retinal circulation strongly supports the claim that retinovitreal new vessels can be produced in the mouse model, given the appropriate experimental conditions. 17 The precise role of individual extracellular matrix components in normal and abnormal retinal vascularization is yet to be determined.…”

Section: Discussionsupporting

confidence: 81%

Laminin‐like Immunoreactivity in Induced New Vessels in Kitten and Mouse Eyes

Gole

Belford

Rush

1987

Australian and New Zealand Journal of Ophthalmology

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Laminin is a basement membrane glycoprotein, widely distributed in body tissues. It has been found on capillary basement membranes in both developing and adult retinal vessels. Oxygen-induced retinopathy was produced in kittens and mice by exposing them to a high oxygen environment during the newborn period. When the animals developed a proliferative retinopathy after a period of survival in room air, they were sacrificed and the eyes enucleated and embedded in paraffin wax. Cross-sections were cut and de-waxed. After preliminary digestion with pepsin, we used a fluorescein isothiocyanate (FlTC)-labelled double antibody technique to identifv laminin-like immunoreactivity (LLlRj. LLlR was found on both intravitreal and intraretinal new vessels in the kitten and mouse, indicating that it is probably secreted by endothelial cells during the formation of pathological new vessels.Key words: Laminin, retinal vessels, angiogenesis, neovascularization, basement membrane, immunohistochemistry. There is an increasing awareness that components of the extracellular matrix may affect endothelial cell behaviour.1.2 Laminin is a noncollagenous glycoprotein (MW = 1 x lo6) found in the extracellular m a t r i~,~.~ and is thought to be involved in the attachment of endothelial, epithelial, and tumour cells to basement membrane^.^-' Endothelial cells in culture secrete l a m i~~i n .~,~ Using immunohistochemical methods on cross-sections, laminin-like immunoreactivity (LLIR) has been reported on the retinal vessels of adult bovine and human Using a whole mount technique, we have previously reported that LLIR is present on the developing retinal vessels of the newborn mouse, on the retinal vessels of the adult rat and mouse, on retracted capillaries, and that LLIR encases pericytes. 12. 13 There is little known concerning the extracellular matrix of pathologically induced new vessel^.'^ Using an immunohistochemical technique on cross-sections of the retina, we have demonstrated the presence of LLIR on induced new vessels in the kitten and mouse models of oxygen-induced retinopathy. METHODS Induction of Oxygen-induced RetinopathyA litter of three-day-old kittens was placed in a ChambeF and exposed to an 80% oxygen environment for 96 hours. On day 14 or day 21 of life, when the kittens exhibited a florid proliferative retinopathy,16 they were sacrificed by an overdose of intraperitoneal pentobarbitone. The eyes were enucleated and fixed in 10% buffered formalin. Routine embedding in paraffin wax was performed and 5 pm crosssections were cut. Newborn mice were placed in a perspex chamber measuring 16 x 16 x 22 cm and exposed to a 98% oxygen environment for five days, according to the protocol described by Ashton." On day ten of life, they were sacrificed by halothane overdosage and the eyes were enucleated, fixed in 10% buffered formalin, and embedded in paraffin wax. Cross-sections were cut and processed as for the kitten material. Immunohistochemical MethodImmunohistochemical localization o f laminin was achieved usin...

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Section: Discussionsupporting

confidence: 81%

Laminin‐like Immunoreactivity in Induced New Vessels in Kitten and Mouse Eyes

Gole

Belford

Rush

1987

Australian and New Zealand Journal of Ophthalmology

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“…[45][46][47][48][49] Could macrophages lack in the vitreous of ND mice? The presence of vitreal macrophages was investigated using lectin and the F4/80 antibody.…”

Section: Discussionmentioning

confidence: 99%

Norrie Gene Product Is Necessary for Regression of Hyaloid Vessels

Ohlmann

Adamek

Lütjen–Drecoll

2004

Invest. Ophthalmol. Vis. Sci.

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“…The results with H R P must be considered provisional because low flow in blind-ended neovascular buds may have been responsible for the generally low concentrations of H R P we often observed in the vitreous capillaries. These results provide further evidence that fenestrated endothelium and/or open junctions are not invariable accompaniments of intravitreal neovascularization [5,6]. There are no published studies which describe the ultrastructure of the vitreous new vessels in the mouse model at various ages, so it may be that fenestrated endothelium and/or open junctions occur at an earlier stage in the evolution of the neovascular process than day 10 of life when our study was performed.…”

Section: Discussionmentioning

confidence: 48%

The mouse model of oxygen-induced retinopathy: A suitable animal model for angiogenesis research

1990

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Newborn mice exposed to high (greater than 98%) ambient oxygen during the newborn period and subsequently removed to room air will develop a proliferative retinopathy which mimics the neovascular component of acute retinopathy of prematurity. In this paper, we report preliminary ultrastructural findings on the vitreous new vessels in the mouse model of oxygen-induced retinopathy, and argue that the model is appropriate for research on non surgical treatments for ROP in particular and angiogenesis in general.

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Scanning electron microscopic studies of the hyaloid vascular system in newborn mice exposed to O2 and CO2

Cited by 21 publications

References 6 publications

Laminin‐like Immunoreactivity in Induced New Vessels in Kitten and Mouse Eyes

Laminin‐like Immunoreactivity in Induced New Vessels in Kitten and Mouse Eyes

Norrie Gene Product Is Necessary for Regression of Hyaloid Vessels

The mouse model of oxygen-induced retinopathy: A suitable animal model for angiogenesis research

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