Scanning electron microscopic examination of pocket wall epithelium and associated plaque in localized juvenile periodontitis

Abstract

The purpose of this investigation was to describe the morphologic characteristics of epithelial-associated plaque in periodontal pockets of patients with localized juvenile periodontitis (LJP). 25 tissue specimens obtained from 10 adolescent patients were examined by scanning electron microscopy. Specimens were evaluated to determine the following specific features: (1) distribution of microbial colonies on the epithelial surface; (2) topographic features of pocket epithelium associated with microbial colonies; and (3) the predominante microbial morphologic types comprising the colonies. Observations made during this investigation revealed the following. (1) The surface epithelium in the coronal one-third of the pocket wall was essentially healthy in appearance and exhibited no distinct microbial colonies or unusual topographic features. (2) The surface epithelium in the middle one-third area featured randomly-dispersed microbial colonies consisting of 3 major morphotypes: cocci, bacilli and coccobacilli. Also in this zone, there was morphologic evidence of microbial penetration of the epithelial barrier along intercellular spaces. (4) The apical one-third zone was characterized by lymphocytic infiltration, epithelial cavitation and ulcerations, and singular organisms entrapped in fibrin meshworks. There were no distinct microbial colonies in this zone, although individual spirochetes, fusiforms, filamentous organisms, and short and long rods were observed.

Potential role for Streptococcus gordonii‐derived hydrogen peroxide in heme acquisition by Porphyromonas gingivalis

“…67 In this regard, H 2 O 2 at concentrations of 100-200 μmol L −1 can diffuse up to 200 μm through a mono-culture biofilm of S. gordonii. 68 It is also important to note that ultrastructural studies on the diseased periodontal pocket epithelium show that erythrocytes tend to associate with bacterial aggregates in dental plaque, 69 and that S. gordonii possesses a cell-surface adhesin that binds to erythrocytes. 70 Oxidation of oxyHb to metHb mediated by H 2 O 2 is a complex process involving entry of H 2 O 2 into the heme pocket.…”

Clinical and microbiological rationale for laser removal of infected epithelia in patients with periodontitis

“…Значительная часть исследований микробиоце-ноза биотопа пародонтального кармана (ПК) опирается на изучение раневого экссудата или содержимого ПК. Од-нако микробиологическое исследование десневых био-птатов (ДБ), содержащих микроорганизмы в состоянии адгезии к эпителию ПК и инвазивные формы, также акту-ально [8,9]. В зарубежной литературе в отношении дан-ной части микробиоты биотопа ПК применяют разную терминологию: «эпителиально ассоциированные» [9], «внутриклеточные» [5], «прикрепленные, инвазивные» [4,7,8], «микроорганизмы биоптата» [6].…”

“…Од-нако микробиологическое исследование десневых био-птатов (ДБ), содержащих микроорганизмы в состоянии адгезии к эпителию ПК и инвазивные формы, также акту-ально [8,9]. В зарубежной литературе в отношении дан-ной части микробиоты биотопа ПК применяют разную терминологию: «эпителиально ассоциированные» [9], «внутриклеточные» [5], «прикрепленные, инвазивные» [4,7,8], «микроорганизмы биоптата» [6]. Внутриклеточные микроорганизмы характеризуются большей устойчиво-стью к традиционной антимикробной терапии [5] и могут поддерживать циклическое течение ХГП [4,8].…”

Association of P2RX7 functional variants with localized aggressive periodontitis

“…Periodontal tissue destruction occurs when the body mounts an unbalanced immune response to the presence of bacteria under the gingiva. LAP exhibits a localized inflammatory reaction that is particularly rapid and severe, with infiltration of immune cells into the tissue . Without treatment, the progression of LAP often leads to continued bone resorption around the affected teeth and eventual tooth loss.…”