Scanning electron microscope evaluation of chlorhexidine gel and liquid associated with sodium hypochlorite cleaning on the root canal walls

Valera, Márcia Carneiro; Chung, Adriana; Menezes, Márcia Maciel; Fernandes, Carlos Eduardo Fernandes; Carvalho, Cláudio Antônio Talge; Camargo, Samira Esteves Afonso; Camargo, Carlos Henrique

doi:10.1016/j.tripleo.2010.04.051

Cited by 15 publications

(17 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…From the review of the literature, it transpires that mixing NaOCl with liquid CHX results in the instant formation of a flocculate or precipitate (41)(42)(43)(44)(45)(46)(47)(48)(49). Basrani et al (41) looked into the minimum NaOCl concentration required to form a precipitate when mixed with 2% CHX (41).…”

Section: Interactions Between Naocl and Chxmentioning

confidence: 99%

“…Several investigations have been undertaken to elucidate the chemical composition of the flocculate produced by the association of NaOCl with CHX (41)(42)(43)(44)(45)(47)(48)(49). Different proportions and concentrations of NaOCl (0.5%, 2.5%, and 5%) and CHX (0.2%-2%) have been mixed, which results in the formation of a brownish flocculate evident when the solutions make contact with each other; atomic absorption spectrophotometry showed the presence of Ca, Fe, and Mg (42).…”

Section: Interactions Between Naocl and Chxmentioning

confidence: 99%

“…Three studies have evaluated the cleaning efficacy after irrigation with CHX containing solutions (44,47,48). Bui et al (47) investigated the influence of irrigation on debris removal and patent dentinal tubules using 5.25% NaOCl and 2% CHX ex vivo and analyzed it with an environmental scanning electron microscope (47).…”

Section: Interactions Between Naocl and Chxmentioning

confidence: 99%

“…There was no difference in remaining debris and a reduction in number of patent dentinal tubules in the coronal and middle third between the 2 test groups. A scanning electron microscopic investigation into the percentage of open and closed tubules after root canal instrumentation on human teeth using 2.5% NaOCl and 2% CHX in liquid or gel forms, intercalated by physiologic saline, with half of the experimental groups receiving a final flush with 17% EDTA was performed by Valera et al (48). Their results indicated that 2% CHX gel produced the largest amount of open dentinal tubules, whereas 2% CHX liquid presented the worst result.…”

Section: Interactions Between Naocl and Chxmentioning

confidence: 99%

“…NaOCl and CHX: to prevent the formation of a precipitate associated with CHX and NaOCl interactions, a rinse with intermediate solutions after NaOCl has been suggested. They include saline (48); water (47,48); alcohol (42,47); isopropyl alcohol (44); or a demineralizing solution, which can be CA (34) or EDTA (42). Finally, if the flocculate is formed, then acetic acid can be used to dissolve the precipitate (41).…”

Section: Preventive Strategiesmentioning

confidence: 99%

See 4 more Smart Citations

Antagonistic Interactions between Sodium Hypochlorite, Chlorhexidine, EDTA, and Citric Acid

Rossi‐Fedele

Doğramacı

Guastalli

et al. 2012

Journal of Endodontics

122

View full text Add to dashboard Cite

Section: Interactions Between Naocl and Chxmentioning

confidence: 99%

Section: Interactions Between Naocl and Chxmentioning

confidence: 99%

Section: Interactions Between Naocl and Chxmentioning

confidence: 99%

Section: Interactions Between Naocl and Chxmentioning

confidence: 99%

Section: Preventive Strategiesmentioning

confidence: 99%

See 3 more Smart Citations

Antagonistic Interactions between Sodium Hypochlorite, Chlorhexidine, EDTA, and Citric Acid

Rossi‐Fedele

Doğramacı

Guastalli

et al. 2012

Journal of Endodontics

122

View full text Add to dashboard Cite

Ultrastructural analysis of the root canal walls after simultaneous irrigation of different sodium hypochlorite concentration and 0.2% chlorhexidine gluconate

Gasic

Popović

Živković

et al. 2012

Microscopy Res & Technique

View full text Add to dashboard Cite

To determine whether sodium hypochlorite (NaOCl) with 0.2% chlorhexidine gluconate (CHX) leads to colour change and precipitate formation, and to ultrastructurally analyse the dentine surface after simultaneous irrigation with 0.5% NaOCl and 0.2% CHX. Four tubes in which 5ml of different NaOCl concentrations and 5ml 0,2% CHX were placed, were observed every 15 minutes in the first two hours and after 7 days. Mixture solutions were centrifuged at 800 rpm/4 min. A precipitate is observed under light-microscopy. Thirty-five single-rooted teeth were instrumented using crown-down technique with irrigation: Positive control: distilled water, Negative control: 0,5% NaOCl+15% EDTA, Experimental group: identical to the negative control, then canals were treated with 0.5% NaOCl+0.2% CHX. The longitudinal root sections were observed under scanning-electron-microscopy. The amount of debris was assessed with 5/score-system, and the results were analysed using the Mann-Whitney U test (p < 0.001). Change in colour was noticed immediately after the merger and it did not change with time. Mixture solutions showed considerable turbidity, but precipitate was observed only after centrifugation. Statistical analysis showed a significant difference in the dentinal wall purity when comparing the cervical and middle root canal thirds between the experimental and negative control group. The difference was also observed between the cervical and middle thirds compared to the apical third of the root canal within these groups. Interaction between NaOCl and CHX, as well as the creation of precipitates, depends not only on the concentration of NaOCl, but also on the concentration of CHX.

show abstract

Residues of different gel formulations on dentinal walls: A SEM/EDS analysis

Nascimento

Busanello

Só

et al. 2015

Microsc. Res. Tech.

View full text Add to dashboard Cite

The aim of this study was to evaluate the presence of residues of sodium hypochlorite gel, chlorhexidine gel, and EDTA gel on dentinal walls after canal preparation through chemical SEM- elemental chemical microanalysis (EDS) analysis. Forty-eight single-rooted teeth were selected. They had their crowns sectioned and were instrumented with a reciprocating system. The canals were irrigated with 5 mL of saline solution during root canal preparation. After instrumentation, the root canals were irrigated with 3 mL 17% EDTA followed by 1 min of ultrasonic passive activation (3× 20 sec) to remove the smear layer, and then irrigated with 3 mL of saline solution. The specimens were randomized into three groups (n = 12) according to the chemical substance that filled the root canal for 30 min: GI: 5.5% sodium hypochlorite gel; GII: 2% chlorhexidine gel; GIII: 24% EDTA gel; Negative control group: no substance was used. Then, the root canals were irrigated with 6 mL of saline solution followed by 1 min of ultrasonic passive activation (3× 20 sec). After ultrasonic activation, the canals were irrigated with 2 mL saline. The roots were sectioned, and the percentage of each chemical element present in the samples was analyzed through chemical SEM-EDS microanalysis. All experimental groups showed a significantly higher percentage of chemical elements (Na and/or Cl) than the control group (P < 0.03). This in vitro study has shown that, regardless of chemical solutions used even after the final irrigation protocol, chemical residues of different substances remained attached to the root canal walls.

show abstract

Scanning electron microscope evaluation of chlorhexidine gel and liquid associated with sodium hypochlorite cleaning on the root canal walls

Cited by 15 publications

References 18 publications

Antagonistic Interactions between Sodium Hypochlorite, Chlorhexidine, EDTA, and Citric Acid

Antagonistic Interactions between Sodium Hypochlorite, Chlorhexidine, EDTA, and Citric Acid

Ultrastructural analysis of the root canal walls after simultaneous irrigation of different sodium hypochlorite concentration and 0.2% chlorhexidine gluconate

Residues of different gel formulations on dentinal walls: A SEM/EDS analysis

Contact Info

Product

Resources

About