Scanning confocal fluorescence microscopy for single molecule analysis of nucleotide excision repair complexes

Segers-Nolten, Gezina M.J.; Wyman, Claire; Wijgers, Nils; Vermeulen, Wim; Lenferink, Aufrid T.M.; Hoeijmakers, J.H.J.; Greve, Jan; Otto, Cornelis

doi:10.1093/nar/gkf599

Cited by 31 publications

(30 citation statements)

References 38 publications

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“…The home-built SFCM with imaging capability consisted of an Ar + /Kr + mixed gas laser (Coherent, Innova 70 spectrum, Santa Clara, CA) and an inverted optical microscope and has been described elsewhere [67,68]. It was redesigned to accommodate on-line quantitative FCS and steady-state fluorescence anisotropy measurements as well as single-molecule spectral measurements.…”

Section: Microscope Configurationmentioning

confidence: 99%

“…The SFCM was described elsewhere [67,68]. A detailed description of the set up is presented in Chapter 2 (section 2.2).…”

Section: Single-molecule Fluorescence Confocal Microscopementioning

confidence: 99%

“…The main configuration of this SFCM has been described elsewhere [67,68], and a recent development of on-line quantitative FCS analysis has been introduced in Ref. [69].…”

Section: Single-molecule Fluorescence Confocal Microscopementioning

confidence: 99%

“…The main configuration of this SFCM has been presented elsewhere [67,68], and the SFCM has been further developed with the on-line quantitative FCS, which has been used for the study of soluble protein stability in Chapter 5, and the combined FCS and SFA techniques for DNA repair proteins binding to DNA in Chapter 6. In both Chapters 5-6, FCS and SFA were performed on-line in the fluorescent intensity time traces measured in sample solutions at a fixed position or by sequentially scanning a few preset positions.…”

Section: Multiparameter Fluorescence Imaging Microscopymentioning

confidence: 99%

“…Nevertheless, it should be noted that significant groundwork had been done in building the microscope [67,68] prior to the start of this thesis. A fluorescent spectrometer with single-molecule sensitivity, on-line quantitative fluorescence correlation spectroscopy (FCS) and fluorescence cross-correlation spectroscopy (FCCS), and steady-state fluorescence anisotropy (SFA) as well as the inverted configuration of the microscope are most recent developments.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Quantitative fluorescence nanospectroscopy of nucleotide excision repair : from single molecules to cells

Zhang¹

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Section: Microscope Configurationmentioning

confidence: 99%

“…The SFCM was described elsewhere [67,68]. A detailed description of the set up is presented in Chapter 2 (section 2.2).…”

Section: Single-molecule Fluorescence Confocal Microscopementioning

confidence: 99%

“…The main configuration of this SFCM has been described elsewhere [67,68], and a recent development of on-line quantitative FCS analysis has been introduced in Ref. [69].…”

Section: Single-molecule Fluorescence Confocal Microscopementioning

confidence: 99%

Section: Multiparameter Fluorescence Imaging Microscopymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Quantitative fluorescence nanospectroscopy of nucleotide excision repair : from single molecules to cells

Zhang¹

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Nucleic Acid and Protein Single Molecule Detection and Characterization

Greulich

2006

Encyclopedia of Molecular Cell Biology and Molecular Medicine

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Single‐Molecule Studies on DNA and RNA

Greulich¹

2005

ChemPhysChem

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DNA and RNA are the most individual molecules known. Therefore, single-molecule experiments with these nucleic acids are particularly useful. This review reports on recent experiments with single DNA and RNA molecules. First, techniques for their preparation and handling are summarised including the use of AFM nanotips and optical or magnetic tweezers. As important detection techniques, conventional and near-field microscopy as well as fluorescence resonance energy transfer (FRET) and fluorescence correlation spectroscopy (FCS) are touched on briefly. The use of single-molecule techniques currently includes force measurements in stretched nucleic acids and in their complexes with binding partners, particularly proteins, and the analysis of DNA by restriction mapping, fragment sizing and single-molecule hybridisation. Also, the reactions of RNA polymerases and enzymes involved in DNA replication and repair are dealt with in some detail, followed by a discussion of the transport of individual nucleic acid molecules during the readout and use of genetic information and during the infection of cells by viruses. The final sections show how the enormous addressability in nucleic acid molecules can be exploited to construct a single-molecule field-effect transistor and a walking single-molecule robot, and how individual DNA molecules can be used to assemble a single-molecule DNA computer.

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Scanning confocal fluorescence microscopy for single molecule analysis of nucleotide excision repair complexes

Cited by 31 publications

References 38 publications

Quantitative fluorescence nanospectroscopy of nucleotide excision repair : from single molecules to cells

Quantitative fluorescence nanospectroscopy of nucleotide excision repair : from single molecules to cells

Nucleic Acid and Protein Single Molecule Detection and Characterization

Single‐Molecule Studies on DNA and RNA

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