Scanning and transmission electron microscopic study of visceral and parietal peritoneal regions in the rat

Michailova, K.; Wassilev, W; Wedel, Thilo

doi:10.1016/s0940-9602(99)80040-5

Cited by 51 publications

(46 citation statements)

References 28 publications

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“…In the control ileum these cells had an elongated outline, approximately aligned with each other and with the longer axis parallel to the circumference of the intestine (Fig. 5 a), as already described in the literature (Andrews & Porter, 1973 ;Michailova et al 1999). Their surface displayed abundant microvilli, which were not densely packed (Table) and were more abundant in the central region of the cell (Fig.…”

Section: supporting

confidence: 68%

Hypertrophy of mucosa and serosa in the obstructed intestine of rats

Bertoni¹,

Gabella²

2001

Journal of Anatomy

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After a surgically induced partial obstruction of the small intestine (ileum) in adult rats there is an accumulation of ingesta and a progressive enlargement of the lumen accompanied by wall thickening : over a period of 2-3 wk the circumference of the hypertrophic intestine increases by a factor of 2n7 and the thickness of the musculature increases more than threefold, while the length of the ileum (measured at the mesenteric attachment) remains unchanged. The villi become markedly larger and more elongated in the circumferential direction, and have a greater separation between one another. The number of villi per unit surface is markedly reduced but the number of villi per unit length of ileum, whilst appearing to show a small increase, was not significantly altered. The component epithelial cells (absorptive cells) appear unchanged in morphology and size (height). The microvilli of the epithelial cells have the same appearance, size (height) and packing density in the control and the hypertrophic ileum. Glands of Lieberku$ hn, Peyer's patches and single lymphatic follicles constituting the Peyer's patches are significantly increased in size in the hypertrophic intestine. The serosal surface of the hypertrophic ileum, in spite of the great expansion, remains regularly covered by mesothelial cells ; these are much larger than in the controls and have an altered distribution of their microvilli.Key words : Intestinal villi ; microvilli ; Peyer's patches ; mesothelium ; hypertrophy ; intestinal obstruction. When the flow of ingesta along the small intestine is impaired, as in the presence of a localised partial obstruction of the lumen, the intestine on the oral side of the obstruction becomes distended and hypertrophic. Enlargement of the lumen and distension of the wall are mechanical effects of the accumulation of ingesta. In contrast, hypertrophy is an active process of growth of the intestinal wall, mainly the musculature, the effect of which is that the wall, in spite of being very distended, is not thinner than in controls but conspicuously thicker. The occurrence of ileal hypertrophy, its relevance to medical conditions (such as obstructions due to pathologic growths or malformations), its scientific significance as a process of growth and adaptation of mature tissues, have been known for a long time. Herczel (1886) was probably the first to describe the structure of hypertrophic intestine, and the process has been analysed in Correspondence to Dr

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Section: supporting

confidence: 68%

Hypertrophy of mucosa and serosa in the obstructed intestine of rats

Bertoni¹,

Gabella²

2001

Journal of Anatomy

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“…It is essential for homeostatic maintenance to provide the peritoneal integrity, which can be affected during laparoscopic surgery, where mesothelial cells detach from the basal membrane and expose the basal membrane [1,37,38]. Several studies based on an animal model have showed that the bulging up of mesothelial cells occurs almost immediately after the initiation of a pneumoperitoneum.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of peritoneal tissue by means of differential scanning calorimetry (DSC)

Torres¹,

Trębacz²,

Chróścicki³

et al. 2012

Folia Histochem Cytobiol.

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Abstract:Abdominal surgeries alter the integrity of the peritoneal layer and cause imbalances among immunological, inflammatory and angiogenic mechanisms within the tissue. During laparoscopic procedures a protective function of the peritoneal layer can be disturbed by the gas used to create a pneumoperitoneum. The aim of this study was to characterize peritoneal tissue by means of differential scanning calorimetry (DSC) as a reference for future investigations on the influence of surgical procedures on the physicochemical state of the peritoneum. Thirty-seven patients participated in the study. Patients were divided into three groups according to the type of surgery: group H -patients who underwent hernia repair; group Ch -patients who underwent laparoscopic cholecystectomy; and group C -patients operated due to rectal cancer. It was observed that onset temperature (T o ), denaturation temperature (T m ) and change of enthalpy (DH) during thermal denaturation of peritoneal collagen in were significantly different for these three groups of patients. The mean values of onset temperature (T o ) and denaturation temperature (T m ) in group H were significantly lower, while DH in this group was significantly higher than in the two other groups (Ch and C). This preliminary study does not answer whether the differences in collagen denaturation found in peritoneal tissue from different groups of patients resulted from a different inherent state of the tissue, or from surgical procedures. However, the results suggest that DSC is an appropriate method to study subtle changes in the physicochemical condition of the peritoneum using small samples obtained during surgical procedures.

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“…However, although the concept of contamination propagation remains, it has not been clinically verified 6 and cannot not applied to diffuse peritonitis when the contamination already involved the entire peritoneum. The parietal peritoneum consists of a single layer of mesothelial cells and a conjunctive tissue layer containing cells, blood vessels, lymphatic vessels and nervous fibers 8,9 , what may be physically and biologically similar to the dermis that once injured and contaminated, needs the purulent secretion, strange bodies, dead tissues and other impurities to be washed off 3 . Once recognized that PL was vital for the treatment of the secondary peritonitis 3,4,6,23 , researchers added to the saline solution substances such as antibiotics 2,11,24 , antimicrobial agents 12 , chemotherapic agents 13 , hypertonic glucose 10% 14 and immunoglobulin 15 with the objective of increasing the procedure efficiency.…”

Section: Introductionmentioning

confidence: 99%

Histopathologic evaluation of the peritoneum exposed to heat shock: experimental study in rats

et al. 2007

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PURPOSE: To evaluate histopathologic alterations of the peritoneum exposed to heat shock. METHODS: Sixty rats were randomly distributed into 6 groups: Heat Shock (HS), High Temperature (HT), Body Temperature (BT), Temperature 0oC (TZ), Sham (SH) and Control (CG) with 10 animals each. The peritoneal cavity of animals from groups HS, HT, BT and TZ was irrigated with NaCl solution 0.9% at temperatures 50ºC, 0ºC, 50ºC, 37ºC and 0ºC, respectively. For animals from group SH, the procedures were simulated and those from group CG, laparotomy and biopsies were conducted. Twenty-four hours later, biopsies of the peritoneum for exams under light and electronic microscopy were performed. RESULTS: Edema was found in groups HS 80%, HT 60%, BT 30% TZ 70%, SH 40% and CG 30%. Vascular congestion was found in groups HS 20%, HT 30%, BT 10% and TZ 20%. Erythrocyte extravasation was found in groups HT 60% and SH 10%. Mesothelium destruction was found in 100% of specimens from groups HS, HT, BT, TZ, SH and CG 90%. Necrosis was found in groups HS 30%, HT 20% and BT 10%. The mean peritoneal thickness ranged from 42.26 µm (TZ) to 26.42 µm (CG). CONCLUSION: The heat shock caused no deaths, but promoted significant peritoneal edema without affecting the other histopathologic indicatives.

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Scanning and transmission electron microscopic study of visceral and parietal peritoneal regions in the rat

Cited by 51 publications

References 28 publications

Hypertrophy of mucosa and serosa in the obstructed intestine of rats

Hypertrophy of mucosa and serosa in the obstructed intestine of rats

Evaluation of peritoneal tissue by means of differential scanning calorimetry (DSC)

Histopathologic evaluation of the peritoneum exposed to heat shock: experimental study in rats

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