Scale-Up Seed Decontamination Process to Inactivate<i>Escherichia coli</i>O157:H7 and<i>Salmonella</i>Enteritidis on Mung Bean Seeds

Bari, Md. Latiful; Enomoto, Katsuyoshi; Nei, Daisuke; Kawamoto, Shinnichi

doi:10.1089/fpd.2009.0389

Cited by 15 publications

(6 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was followed by a combination study with reduced hot water temperature and time, followed by soaking in chlorine. In that study 4 , we reported that hot water treatment at 85°C for 40 seconds followed by dipping in cold water for 30 sec and soaking in 2000-ppm chlorine water could achieve complete elimination of E. coli O157:H7 and Salmonella Enteritidis in mung bean seeds without affecting the germination yield after the treatment. Finally, we evaluated the scale-up study results 3 with an actual pasteurization machine (SB-1300: Daisey Mechinary Co. Ltd) for disinfecting food poisoning bacteria, E. coli O157:H7, Salmonella, and non-pathogenic E. coli as a surrogate for practical tests.…”

Section: Successful Seed Decontamination Methodsmentioning

confidence: 99%

Development of Effective Seed Decontamination Technology to Inactivate Pathogens on Mung Bean Seeds and Its Practical Application in Japan

Bari

Enomoto²,

Nei

et al. 2011

JARQ

Self Cite

View full text Add to dashboard Cite

The majority of seed sprout-related outbreaks has been associated with Escherichia coli O157:H7 and Salmonella. Therefore, it is necessary to find an effective method to inactivate possible pathogenic bacterial populations on the seeds prior to sprouting. In general, sanitizing is more effective in reducing contamination on seeds than on sprouts. A successful seed decontamination treatment must inactivate microbial pathogens while preserving seed viability, germination, and vigor. Seeds vary in sensitivity to antimicrobial agents and other treatments, which determine how well they germinate and grow after treatment. In addition, a treatment that is effective for one type of seed may not be applicable to all types of seeds. Seeds vary in surface features, which may influence how well an antimicrobial agent can access and inactivate pathogens on or in the seed. The use of a number of physical, non-thermal processing technologies, alone or in combination with antimicrobial chemicals, could be useful for seed decontamination. Until now, hot water treatment at 85°C for 40 seconds followed by cooling in cold water for 30 sec and soaking in chlorine water was found effective in inactivating pathogens while preserving seed viability, germination, and vigor. Therefore, hot water treatment could be an effective seed decontamination method for mung bean seeds intended for sprout production.

show abstract

Section: Successful Seed Decontamination Methodsmentioning

confidence: 99%

Development of Effective Seed Decontamination Technology to Inactivate Pathogens on Mung Bean Seeds and Its Practical Application in Japan

Bari

Enomoto²,

Nei

et al. 2011

JARQ

Self Cite

View full text Add to dashboard Cite

show abstract

“…This treatment was effective for reducing pathogens to below detectable levels after 24 h of enrichment and maintained 95% sprout yield after 72 h. However, treatment at higher water temperature for a shorter times was not as effective (10). Because recovery of injured cells often increases and germination is altered when the treatment sample size increases, this method was further validated on a commercial scale with a 300-kg sample (9). However, when tested on mung bean seeds from a different source, the combination treatment achieved > 5 log CFU/g reduction but was not able to completely eliminate Salmonella from detection (118).…”

Section: Physical Interventions: Thermal Inactivationmentioning

confidence: 99%

Current Intervention Strategies for the Microbial Safety of Sprouts

Sikin

Zoellner

Rizvi

2013

Journal of Food Protection

View full text Add to dashboard Cite

Sprouts have gained popularity worldwide due to their nutritional values and health benefits. The fact that their consumption has been associated with numerous outbreaks of foodborne illness threatens the $250 million market that this industry has established in the United States. Therefore, sprout manufacturers have utilized the U.S. Food and Drug Administration recommended application of 20,000 ppm of calcium hypochlorite solution to seeds before germination as a preventative method. Concentrations of up to 200 ppm of chlorine wash are also commonly used on sprouts. However, chlorine-based treatment achieves on average only 1- to 3-log reductions in bacteria and is associated with negative health and environmental issues. The search for alternative strategies has been widespread, involving chemical, biological, physical, and hurdle processes that can achieve up to 7-log reductions in bacteria in some cases. The compilation here of the current scientific data related to these techniques is used to compare their efficacy for ensuring the microbial safety of sprouts and their practicality for commercial producers. Of specific importance for alternative seed and sprout treatments is maintaining the industry-accepted germination rate of 95% and the sensorial attributes of the final product. This review provides an evaluation of suggested decontamination technologies for seeds and sprouts before, during, and after germination and concludes that thermal inactivation of seeds and irradiation of sprouts are the most practical stand-alone microbial safety interventions for sprout production.

show abstract

“…The survival of E. coli O157:H7 was confirmed according to Bari et al (2010). The homogenized mixture of 10 g of seeds with 90 mL of peptone water in a stomacher bag was kept in an incubator at 378C for 24 h for enrichment, and the homogenates (0.1 mL) were plated onto TSAR and SMACR.…”

Section: Detection Of Surviving Pathogens In Treated Seedsmentioning

confidence: 99%

Combined Effect of Low-Dose Irradiation and Acidified Sodium Chlorite Washing onEscherichia coliO157:H7 Inoculated on Mung Bean Seeds

Nei

Bari²,

Inatsu³

et al. 2010

Foodborne Pathogens and Disease

Self Cite

View full text Add to dashboard Cite

The effect of low-dose irradiation (0.75 and 1.5 kGy) in combination with acidified sodium chlorite (ASC) on the reduction of Escherichia coli O157:H7 on mung bean seeds was examined. Washing with ASC (0.2, 0.5, 0.8, and 1.2 g/L sodium chlorite and 1.0 g/L citric acid) for 2 h reduced the E. coli O157:H7 population from 5.2 to 2.3-3.3 log CFU/g, depending on the concentrations of sodium chlorite. Gamma ray irradiation at 0.75 and 1.5 kGy resulted in reductions of about 1.8 and 2.8 log CFU/g, respectively. Therefore, a single treatment with ASC washing or gamma ray irradiation at 0.75 or 1.5 kGy could not achieve the complete elimination of E. coli O157:H7 on mung bean seeds. Conversely, low-dose irradiation (0.75 and 1.5 kGy) followed by washing with ASC (0.5-1.2 g/L) reduced the population of E. coli O157:H7 to below the detection limit (<1 log CFU/g). However, E. coli O157:H7 was detected in most samples in the enrichment and germination studies. When the treatment order was reversed (ASC washing followed by low-dose irradiation), the E. coli O157:H7 population was also observed to be below the detection limit. Under this treatment, fewer samples (16.7%) were shown to be positive in the enrichment and germination studies, and complete elimination was not achieved. The germination rates of mung bean seeds were not affected by ASC washing and gamma irradiation; however, the yield and length of sprouts were decreased by gamma irradiation.

show abstract

Scale-Up Seed Decontamination Process to InactivateEscherichia coliO157:H7 andSalmonellaEnteritidis on Mung Bean Seeds

Cited by 15 publications

References 15 publications

Development of Effective Seed Decontamination Technology to Inactivate Pathogens on Mung Bean Seeds and Its Practical Application in Japan

Development of Effective Seed Decontamination Technology to Inactivate Pathogens on Mung Bean Seeds and Its Practical Application in Japan

Current Intervention Strategies for the Microbial Safety of Sprouts

Combined Effect of Low-Dose Irradiation and Acidified Sodium Chlorite Washing onEscherichia coliO157:H7 Inoculated on Mung Bean Seeds

Contact Info

Product

Resources

About