Scaffold Protein JLP Is Critical for CD40 Signaling in B Lymphocytes

Wang, Huiming; Yan, Qi; Yang, Tao; Cheng, Hui; Du, Juan; Yoshioka, Katsuji; Kung, Sam K. P.; Ding, Guohua

doi:10.1074/jbc.m114.618496

Cited by 16 publications

(11 citation statements)

References 44 publications

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“…[ 29 , 30 ] CD40 was initially found in B lymphocytes, which has been suggested to be participated in the activation of B cells and has other key roles. [ 31 ] Apoptosis induction can be inhibited by the interaction between CD40 and their ligands in B lymphocytes [ 32 ] ; but the expression level of CD40 is low under normal physiological conditions, which may be significantly up-regulated under pathological conditions. [ 33 ] Importantly, as the key hub of the inflammatory and immune responses in vivo, the CD40/CD40L system may be responsible for antigen presentation and immune response, and associate significantly with the activation of T lymphocytes and macrophages, thereby exerting critical biological roles in different cell types by the modulation of different signaling pathways.…”

Section: Discussionmentioning

confidence: 99%

Clinical significance of costimulatory molecules CD40/CD40L and CD134/CD134L in coronary heart disease

Chen

Jianhao²,

Zhao³

et al. 2017

Medicine

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The aim of the study was to evaluate the potential role of CD40/CD40 ligand (CD40L) and CD134/CD134 ligand (CD134L) in the development of coronary heart disease (CHD) via the performance of a case-control study.The research objects were 234 cases of CHD patients and 120 cases of well-matched normal controls. Following the separation of peripheral blood mononuclear cells (PBMCs), real-time quantitative PCR (qRT-PCR), Western blot, immunohistochemistry, and flow cytometry were applied for the detection of mRNA levels and expression levels of CD40/CD40L and CD134/CD134L; meanwhile, intercellular adhesion molecule-1 (ICAM-1) and Fas protein mRNA levels were detected using qRT-PCR.There was no statistical difference in the comparison of baseline characteristics between groups, indicating comparability between groups. qRT-PCR and Western blot analysis indicated that CD40/CD40L and CD134/CD134L mRNA and protein expression levels were all increased in the CHD group than those in the control group. Flow cytometry further confirmed the similar tendency. Meanwhile, ICAM-1 and Fas protein mRNA levels were elevated in the CHD group and positively correlated with the above parameters. Furthermore, CD40/CD40L expression rates were negatively correlated with gender and different types of CHD. Meanwhile, CD134/CD134L expressions were also higher in male patients, in patients with family history, previous history of hypertension, diabetes, and cerebrovascular diseases.CD40/CD40L and CD134/CD134L are increased and may have potential correlation with clinical pathological features of patients with CHD. Further in-depth exploration of costimulatory molecules for CHD guidance as well as intrinsic mechanisms are needed combined with in vivo and in vitro experiments.

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Section: Discussionmentioning

confidence: 99%

Clinical significance of costimulatory molecules CD40/CD40L and CD134/CD134L in coronary heart disease

Chen

Jianhao²,

Zhao³

et al. 2017

Medicine

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“…CD40L binding to CD40 leads to CD40 internalization. Engagement of CD40 on murine B cells by sCD40L leads to rapid loss of surface CD40 expression by receptor internalization (16)(17)(18). To test whether CD40L expressed by B cells could engage CD40 on B cells and explain the phenotype observed in SLE African American patients, we cultured purified B cells from NHV with soluble CD40L-isoleucine zipper (CD40L-IZ) or anti-IgM F(ab') 2 .…”

Section: Cd45romentioning

confidence: 99%

B cells from African American lupus patients exhibit an activated phenotype

et al. 2016

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“…The observed decrease in CD40 may seem paradoxical at first given that M-MDSCs also increased B cell production of class-switched Abs. However, this may be explained, at least in part, by the observation that in other systems, CD40 is internalized after ligation (73,74). We have previously published that CD40-CD154 binding between B and CD4 + T cells, respectively, and CD40 signaling through selective TRAF adaptor proteins is required for initiation and progression of LP-BM5-induced pathogenesis (49,(75)(76)(77).…”

Section: Discussionmentioning

confidence: 84%