Scaffold-free and label-free biofabrication technology using levitational assembly in a high magnetic field

Parfenov, Vladislav A.; Kampen, Kenny A. van; Каралкин, П. А.; Koudan, Elizaveta V.; Pereira, Frederico D. A. S.; Petrov, Stanislav V.; Nezhurina, Elizaveta K.; Петров, О. Ф.; Myasnikov, M. I.; Walboomers, Frank; Engelkamp, Hans; Christianen, Peter C. M.; Khesuani, Yusef D.; Moroni, Lorenzo; Mota, Carlos

doi:10.1088/1758-5090/ab7554

Cited by 17 publications

(16 citation statements)

References 38 publications

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“…Previously we have already described the magnetic levitational bioassembly of spherical nonhollow 3D tissue construct from spheroids consisted of a human chondrosarcoma cells. [37] Obtained data confirmed the preservation of cell spheroids viability and their fusion ability in a high magnetic field in the presence of Gd 3+ salts. In the current study, we designed a custom agarose cuvette enabling biofabrication of tubular construct and theoretically more complex geometric forms using the acoustic field.…”

Section: Discussionsupporting

confidence: 62%

“…The distribution of the magnetic field was taken from the experimental data of the previous study, [37] while the magnetic field was varying along the vertical axis. The magnetophoretic force was calculated according to previous settings [37] with the current experimental parameters: relative magnetic permeability of spheroids and medium were sph = 0.999992 and f = 0.999994, respectively, the intensity of the magnetic field was 9.5 T.…”

Section: Numerical Simulation Of Assembly: Calculation Of the Magnetimentioning

confidence: 99%

“…Here, to achieve the magnetic levitational assembly at a low concentration of the paramagnetic medium, we once again used a 50 mm bore, 31 T Bitter magnets in High Field Magnet Laboratory, Radboud University, Nijmegen, the Netherlands as in our previous study. [37] Evaluation of the functional activity for tissue-engineered constructs containing contractile smooth muscle elements is an important stage of their testing at the preclinical stage. To do this, various biological and pharmacological constrictor agents, such as phenylephrine, prostaglandin, and adenine nucleotides, can be used.…”

Section: Introductionmentioning

confidence: 99%

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Biofabrication of a Functional Tubular Construct from Tissue Spheroids Using Magnetoacoustic Levitational Directed Assembly

Parfenov

Koudan

Krokhmal

et al. 2020

Adv Healthcare Materials

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In traditional tissue engineering, synthetic or natural scaffolds are usually used as removable temporal support, which involves some biotechnology limitations. The concept of “scaffield” approach utilizing the physical fields instead of biomaterial scaffold has been proposed recently. In particular, a combination of intense magnetic and acoustic fields can enable rapid levitational bioassembly of complex‐shaped 3D tissue constructs from tissue spheroids at low concentration of paramagnetic agent (gadolinium salt) in the medium. In the current study, the tissue spheroids from human bladder smooth muscle cells (myospheres) are used as building blocks for assembling the tubular 3D constructs. Levitational assembly is accomplished at low concentrations of gadolinium salts in the high magnetic field at 9.5 T. The biofabricated smooth muscle constructs demonstrate contraction after the addition of vasoconstrictive agent endothelin‐1. Thus, hybrid magnetoacoustic levitational bioassembly is considered as a new technology platform in the emerging field of formative biofabrication. This novel technology of scaffold‐free, nozzle‐free, and label‐free bioassembly opens a unique opportunity for rapid biofabrication of 3D tissue and organ constructs with complex geometry.

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Section: Discussionsupporting

confidence: 62%

Section: Numerical Simulation Of Assembly: Calculation Of the Magnetimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biofabrication of a Functional Tubular Construct from Tissue Spheroids Using Magnetoacoustic Levitational Directed Assembly

Parfenov

Koudan

Krokhmal

et al. 2020

Adv Healthcare Materials

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“…Recently, an innovative method was developed to assemble 3D CS spheroids by levitational forces by using low doses of gadobutrol salt [140] . As biological tissues are considered to be diamagnetic, they can be levitated when paramagnetic medium is used.…”

Section: D Culture Methods Of Primary Bone Tumoursmentioning

confidence: 99%

In vitro three-dimensional cell cultures for bone sarcomas

Muñoz-García

Jubelin

Loussouarn

et al. 2021

Journal of Bone Oncology

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Highlights 3D cell cultures present similar drugs resistance features than in vivo tumours. Unlike 2D cell cultures, 3D culture approaches mimic better the tumour microenvironment. Collagen scaffolds result in a better osteosarcoma 3D proliferation than soft hydrogels. Osteosarcoma 3D models constitute an appealing approach for bone mineralisation studies. Combination of microfluidic/bioprinting technology with bone 3D cultures as ideal tools to study bone sarcomas.

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“…First, we failed to find non-toxic gadolinium or its adequate paramagnetic media alternative. Second, we were able to perform label-free magnetic levitational bioassembly in the high magnetic field with significant (more than 100 times) reduction of paramagnetic gadolinium salts[ 24 ]. We also successfully performed a label-free magnetic levitational bioassembly at the microgravity conditions in space[ 25 ].…”

Section: The Main Dilemma Of Label-free Magnetic Levitational Bioasmentioning

confidence: 99%

Scaffold-free, Label-free, and Nozzle-free Magnetic Levitational Bioassembler for Rapid Formative Biofabrication of 3D Tissues and Organs

Parfenov¹,

Petrov²,

Pereira³

et al. 2020

ijb

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Scaffolding is the conceptual framework of conventional tissue engineering. Over the past decade, scaffold-free approaches as a potential alternative to classic scaffold-based methods have emerged, and scaffold-free magnetic levitational tissue engineering (magnetic force-based tissue engineering [Mag-TE]) is a type of this novel tissue engineering strategy. However, Mag-TE is often based on the use of potentially toxic magnetic nanoparticles. Scaffold-free and label-free magnetic levitational bioassembly do not employ magnetic nanoparticles and thus, the potential toxicity of magnetic nanoparticles can be avoided. In this short review, we describe the conceptual foundation of scaffold-free, label-free, and nozzle-free formative biofabrication using magnetic fields as “scaffields.” The design and implementation of “Organ.Aut,” the first commercial magnetic levitational bioassembler, and the potential applications of magnetic bioassembler are discussed as well.

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Scaffold-free and label-free biofabrication technology using levitational assembly in a high magnetic field

Abstract: , C. (2020). Scaffold-free and label-free biofabrication technology using levitational assembly in a high magnetic field. Biofabrication, 12(4), [045022].

Cited by 17 publications

References 38 publications

Biofabrication of a Functional Tubular Construct from Tissue Spheroids Using Magnetoacoustic Levitational Directed Assembly

Biofabrication of a Functional Tubular Construct from Tissue Spheroids Using Magnetoacoustic Levitational Directed Assembly

In vitro three-dimensional cell cultures for bone sarcomas

Scaffold-free, Label-free, and Nozzle-free Magnetic Levitational Bioassembler for Rapid Formative Biofabrication of 3D Tissues and Organs

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